A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis.
The bHLH transcription factor, Hand1 has been implicated in cardiac looping in the mouse, however its function in the developing heart remains unknown. To investigate the mechanism(s) through which Hand1 might function, we screened for potential downstream target genes using representational differe...
| Auteurs principaux: | , , , |
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| Format: | Journal article |
| Langue: | English |
| Publié: |
2002
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| _version_ | 1826306986477617152 |
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| author | Smart, N Hill, A Cross, J Riley, P |
| author_facet | Smart, N Hill, A Cross, J Riley, P |
| author_sort | Smart, N |
| collection | OXFORD |
| description | The bHLH transcription factor, Hand1 has been implicated in cardiac looping in the mouse, however its function in the developing heart remains unknown. To investigate the mechanism(s) through which Hand1 might function, we screened for potential downstream target genes using representational difference analysis. Thymosin beta4 was found to be down-regulated whereas cystatin C and alphaCA were up-regulated in Hand1-null embryoid bodies. Whole-mount in situ hybridisation on wild type embryos (E8.0-E10.5) and Hand1 homozygous-mutant embryos (E8.0) confirmed co-expression of the putative targets with Hand1 in the heart and their aberrant expression in a Hand1-null background. |
| first_indexed | 2024-03-07T06:56:15Z |
| format | Journal article |
| id | oxford-uuid:fe3c8989-48a6-4a7a-8a3a-40ff4df5d215 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:56:15Z |
| publishDate | 2002 |
| record_format | dspace |
| spelling | oxford-uuid:fe3c8989-48a6-4a7a-8a3a-40ff4df5d2152022-03-27T13:34:48ZA differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:fe3c8989-48a6-4a7a-8a3a-40ff4df5d215EnglishSymplectic Elements at Oxford2002Smart, NHill, ACross, JRiley, PThe bHLH transcription factor, Hand1 has been implicated in cardiac looping in the mouse, however its function in the developing heart remains unknown. To investigate the mechanism(s) through which Hand1 might function, we screened for potential downstream target genes using representational difference analysis. Thymosin beta4 was found to be down-regulated whereas cystatin C and alphaCA were up-regulated in Hand1-null embryoid bodies. Whole-mount in situ hybridisation on wild type embryos (E8.0-E10.5) and Hand1 homozygous-mutant embryos (E8.0) confirmed co-expression of the putative targets with Hand1 in the heart and their aberrant expression in a Hand1-null background. |
| spellingShingle | Smart, N Hill, A Cross, J Riley, P A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. |
| title | A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. |
| title_full | A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. |
| title_fullStr | A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. |
| title_full_unstemmed | A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. |
| title_short | A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. |
| title_sort | differential screen for putative targets of the bhlh transcription factor hand1 in cardiac morphogenesis |
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