Evolution of the biosynthesis of two hydroxyacetophenones in plants
Acetophenones are phenolic metabolites of plant species. A metabolic route for the biosynthesis and release of 2 defence-related hydroxyacetophenones in white spruce (Picea glauca) was recently proposed to involve 3 phases: (a) biosynthesis of the acetophenone aglycons catalysed by a currently unkno...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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Wiley
2018
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| _version_ | 1826307182922039296 |
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| author | Parent, GJ Giguère, I Mageroy, M Bohlmann, J MacKay, JJ |
| author_facet | Parent, GJ Giguère, I Mageroy, M Bohlmann, J MacKay, JJ |
| author_sort | Parent, GJ |
| collection | OXFORD |
| description | Acetophenones are phenolic metabolites of plant species. A metabolic route for the biosynthesis and release of 2 defence-related hydroxyacetophenones in white spruce (Picea glauca) was recently proposed to involve 3 phases: (a) biosynthesis of the acetophenone aglycons catalysed by a currently unknown set of enzymes, (b) formation and accumulation of the corresponding glycosides catalysed by a glucosyltransferase, and (c) release of the aglycons catalysed by a glucosylhydrolase (PgβGLU-1). We tested if this biosynthetic model is conserved across Pinaceae and land plant species. We assayed and surveyed the literature and sequence databases for possible patterns of the presence of the acetophenone aglycons piceol and pungenol and their glucosides, as well as sequences and expression of Pgβglu-1 orthologues. In the Pinaceae, the 3 phases of the biosynthetic model are present and differences in expression of Pgβglu-1 gene orthologues explain some of the interspecific variation in hydroxyacetophenones. The phylogenetic signal in the metabolite phenotypes was low across species of 6 plant divisions. Putative orthologues of PgβGLU-1 do not form a monophyletic group in species producing hydroxyacetophenones. The biosynthetic model for acetophenones appears to be conserved across Pinaceae, whereas convergent evolution has led to the production of acetophenone glucosides across land plants. |
| first_indexed | 2024-03-07T06:59:06Z |
| format | Journal article |
| id | oxford-uuid:ff1f35dc-2800-4cf7-b956-0919f758a112 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:59:06Z |
| publishDate | 2018 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:ff1f35dc-2800-4cf7-b956-0919f758a1122022-03-27T13:42:14ZEvolution of the biosynthesis of two hydroxyacetophenones in plantsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ff1f35dc-2800-4cf7-b956-0919f758a112EnglishSymplectic ElementsWiley2018Parent, GJGiguère, IMageroy, MBohlmann, JMacKay, JJAcetophenones are phenolic metabolites of plant species. A metabolic route for the biosynthesis and release of 2 defence-related hydroxyacetophenones in white spruce (Picea glauca) was recently proposed to involve 3 phases: (a) biosynthesis of the acetophenone aglycons catalysed by a currently unknown set of enzymes, (b) formation and accumulation of the corresponding glycosides catalysed by a glucosyltransferase, and (c) release of the aglycons catalysed by a glucosylhydrolase (PgβGLU-1). We tested if this biosynthetic model is conserved across Pinaceae and land plant species. We assayed and surveyed the literature and sequence databases for possible patterns of the presence of the acetophenone aglycons piceol and pungenol and their glucosides, as well as sequences and expression of Pgβglu-1 orthologues. In the Pinaceae, the 3 phases of the biosynthetic model are present and differences in expression of Pgβglu-1 gene orthologues explain some of the interspecific variation in hydroxyacetophenones. The phylogenetic signal in the metabolite phenotypes was low across species of 6 plant divisions. Putative orthologues of PgβGLU-1 do not form a monophyletic group in species producing hydroxyacetophenones. The biosynthetic model for acetophenones appears to be conserved across Pinaceae, whereas convergent evolution has led to the production of acetophenone glucosides across land plants. |
| spellingShingle | Parent, GJ Giguère, I Mageroy, M Bohlmann, J MacKay, JJ Evolution of the biosynthesis of two hydroxyacetophenones in plants |
| title | Evolution of the biosynthesis of two hydroxyacetophenones in plants |
| title_full | Evolution of the biosynthesis of two hydroxyacetophenones in plants |
| title_fullStr | Evolution of the biosynthesis of two hydroxyacetophenones in plants |
| title_full_unstemmed | Evolution of the biosynthesis of two hydroxyacetophenones in plants |
| title_short | Evolution of the biosynthesis of two hydroxyacetophenones in plants |
| title_sort | evolution of the biosynthesis of two hydroxyacetophenones in plants |
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