Assessment of diatom growth using fluorescence imaging
Monitoring the growth of diatom microalgae requires sensitive, fast, and easy-to-use methods for selecting the most productive strains for further research and application. Here we propose a novel, rapid and non-destructive approach for monitoring the viability and growth of diatom colonies using th...
| Main Authors: | , , , , , |
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| Format: | Conference item |
| Language: | English |
| Published: |
IOP Publishing
2021
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| _version_ | 1797107234997534720 |
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| author | Cvjetinovic, J Nozdriukhin, DV Bedoshvili, YD Salimon, AI Korsunsky, AM Gorin, DA |
| author_facet | Cvjetinovic, J Nozdriukhin, DV Bedoshvili, YD Salimon, AI Korsunsky, AM Gorin, DA |
| author_sort | Cvjetinovic, J |
| collection | OXFORD |
| description | Monitoring the growth of diatom microalgae requires sensitive, fast, and easy-to-use methods for selecting the most productive strains for further research and application. Here we propose a novel, rapid and non-destructive approach for monitoring the viability and growth of diatom colonies using the IVIS fluorescence imaging system. The fast-growing Achnanthidium sibiricum diatoms were cultivated in favorable laboratory conditions in a specially designed bioincubator for 100 days. The results showed that the diatoms reached the end of the exponential growth phase after 56 days of culture, after which there was a slight decrease in fluorescence intensity. This method relies on using chlorophyll a fluorescence without any sample preparation and has proved to be successful for monitoring algae growth in an incubator during long-term batch cultivation. This result paves the way towards controlled diatom cultivation aimed at collecting diatom-derived bioactive compounds for various purposes. |
| first_indexed | 2024-03-07T07:11:38Z |
| format | Conference item |
| id | oxford-uuid:ff679f75-05f1-4152-b1a4-c490d6260f56 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:11:38Z |
| publishDate | 2021 |
| publisher | IOP Publishing |
| record_format | dspace |
| spelling | oxford-uuid:ff679f75-05f1-4152-b1a4-c490d6260f562022-06-29T10:54:28ZAssessment of diatom growth using fluorescence imagingConference itemhttp://purl.org/coar/resource_type/c_5794uuid:ff679f75-05f1-4152-b1a4-c490d6260f56EnglishSymplectic ElementsIOP Publishing2021Cvjetinovic, JNozdriukhin, DVBedoshvili, YDSalimon, AIKorsunsky, AMGorin, DAMonitoring the growth of diatom microalgae requires sensitive, fast, and easy-to-use methods for selecting the most productive strains for further research and application. Here we propose a novel, rapid and non-destructive approach for monitoring the viability and growth of diatom colonies using the IVIS fluorescence imaging system. The fast-growing Achnanthidium sibiricum diatoms were cultivated in favorable laboratory conditions in a specially designed bioincubator for 100 days. The results showed that the diatoms reached the end of the exponential growth phase after 56 days of culture, after which there was a slight decrease in fluorescence intensity. This method relies on using chlorophyll a fluorescence without any sample preparation and has proved to be successful for monitoring algae growth in an incubator during long-term batch cultivation. This result paves the way towards controlled diatom cultivation aimed at collecting diatom-derived bioactive compounds for various purposes. |
| spellingShingle | Cvjetinovic, J Nozdriukhin, DV Bedoshvili, YD Salimon, AI Korsunsky, AM Gorin, DA Assessment of diatom growth using fluorescence imaging |
| title | Assessment of diatom growth using fluorescence imaging |
| title_full | Assessment of diatom growth using fluorescence imaging |
| title_fullStr | Assessment of diatom growth using fluorescence imaging |
| title_full_unstemmed | Assessment of diatom growth using fluorescence imaging |
| title_short | Assessment of diatom growth using fluorescence imaging |
| title_sort | assessment of diatom growth using fluorescence imaging |
| work_keys_str_mv | AT cvjetinovicj assessmentofdiatomgrowthusingfluorescenceimaging AT nozdriukhindv assessmentofdiatomgrowthusingfluorescenceimaging AT bedoshviliyd assessmentofdiatomgrowthusingfluorescenceimaging AT salimonai assessmentofdiatomgrowthusingfluorescenceimaging AT korsunskyam assessmentofdiatomgrowthusingfluorescenceimaging AT gorinda assessmentofdiatomgrowthusingfluorescenceimaging |