RNA polymerase I in yeast transcribes dynamic nucleosomal rDNA.
RNA polymerase (Pol) I-transcribed ribosomal genes of budding yeast exist as a tandem array (about 150 repeats) with transcription units separated by spacer sequences. Half of these rDNAs are inactivated by repressive chromatin structure, whereas the rest exist in an open conformation transcribed by...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2007
|
| _version_ | 1826307259918974976 |
|---|---|
| author | Jones, H Kawauchi, J Braglia, P Alen, C Kent, N Proudfoot, N |
| author_facet | Jones, H Kawauchi, J Braglia, P Alen, C Kent, N Proudfoot, N |
| author_sort | Jones, H |
| collection | OXFORD |
| description | RNA polymerase (Pol) I-transcribed ribosomal genes of budding yeast exist as a tandem array (about 150 repeats) with transcription units separated by spacer sequences. Half of these rDNAs are inactivated by repressive chromatin structure, whereas the rest exist in an open conformation transcribed by closely spaced Pol I elongation complexes. Whereas previous studies have suggested that active rDNA is devoid of nucleosomal structure, we demonstrate that active rDNA has nucleosomal structure, according to chromatin immunoprecipitation and biochemical fractionation. Using a yeast strain with reduced numbers of all actively transcribed rDNA repeats, we show that rDNA exists in a dynamic chromatin structure of unphased nucleosomes. Furthermore, it is associated with chromatin-remodeling enzymes Chd1p, Isw1p and Isw2p, whose inactivation causes defects in transcription termination. We suggest that Pol I transcription, like that of Pol II, may be modulated by specific chromatin structures. |
| first_indexed | 2024-03-07T07:00:14Z |
| format | Journal article |
| id | oxford-uuid:ff7d5de4-e600-4dbb-aa4e-18ae694298f5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:00:14Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:ff7d5de4-e600-4dbb-aa4e-18ae694298f52022-03-27T13:45:19ZRNA polymerase I in yeast transcribes dynamic nucleosomal rDNA.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ff7d5de4-e600-4dbb-aa4e-18ae694298f5EnglishSymplectic Elements at Oxford2007Jones, HKawauchi, JBraglia, PAlen, CKent, NProudfoot, NRNA polymerase (Pol) I-transcribed ribosomal genes of budding yeast exist as a tandem array (about 150 repeats) with transcription units separated by spacer sequences. Half of these rDNAs are inactivated by repressive chromatin structure, whereas the rest exist in an open conformation transcribed by closely spaced Pol I elongation complexes. Whereas previous studies have suggested that active rDNA is devoid of nucleosomal structure, we demonstrate that active rDNA has nucleosomal structure, according to chromatin immunoprecipitation and biochemical fractionation. Using a yeast strain with reduced numbers of all actively transcribed rDNA repeats, we show that rDNA exists in a dynamic chromatin structure of unphased nucleosomes. Furthermore, it is associated with chromatin-remodeling enzymes Chd1p, Isw1p and Isw2p, whose inactivation causes defects in transcription termination. We suggest that Pol I transcription, like that of Pol II, may be modulated by specific chromatin structures. |
| spellingShingle | Jones, H Kawauchi, J Braglia, P Alen, C Kent, N Proudfoot, N RNA polymerase I in yeast transcribes dynamic nucleosomal rDNA. |
| title | RNA polymerase I in yeast transcribes dynamic nucleosomal rDNA. |
| title_full | RNA polymerase I in yeast transcribes dynamic nucleosomal rDNA. |
| title_fullStr | RNA polymerase I in yeast transcribes dynamic nucleosomal rDNA. |
| title_full_unstemmed | RNA polymerase I in yeast transcribes dynamic nucleosomal rDNA. |
| title_short | RNA polymerase I in yeast transcribes dynamic nucleosomal rDNA. |
| title_sort | rna polymerase i in yeast transcribes dynamic nucleosomal rdna |
| work_keys_str_mv | AT jonesh rnapolymeraseiinyeasttranscribesdynamicnucleosomalrdna AT kawauchij rnapolymeraseiinyeasttranscribesdynamicnucleosomalrdna AT bragliap rnapolymeraseiinyeasttranscribesdynamicnucleosomalrdna AT alenc rnapolymeraseiinyeasttranscribesdynamicnucleosomalrdna AT kentn rnapolymeraseiinyeasttranscribesdynamicnucleosomalrdna AT proudfootn rnapolymeraseiinyeasttranscribesdynamicnucleosomalrdna |