Multiple SP1 binding sites confer enhancer-independent, replication-activated transcription of HIV-1 and globin gene promoters.
We demonstrate that multiple SP1 protein:DNA binding sites confer enhancer-independent activation on the HIV-1 and globin gene promoters. This activation process can be achieved either by DNA replication of the promoter-containing plasmid or by high concentrations of input plasmid DNA used in the tr...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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1992
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| _version_ | 1797106389657583616 |
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| author | Proudfoot, N Lee, B Monks, J |
| author_facet | Proudfoot, N Lee, B Monks, J |
| author_sort | Proudfoot, N |
| collection | OXFORD |
| description | We demonstrate that multiple SP1 protein:DNA binding sites confer enhancer-independent activation on the HIV-1 and globin gene promoters. This activation process can be achieved either by DNA replication of the promoter-containing plasmid or by high concentrations of input plasmid DNA used in the transfections. In the case of HIV-1, the three SP1 sites adjacent to the promoters TATA box are essential for this activation process. Furthermore, the human beta globin gene, which is normally dependent on a linked enhancer for transcriptional activity, can be made enhancer independent by insertion of SP1 binding sites adjacent to its TATA box. We speculate that (SP1)n-TATA type RNA polymerase II promoters may be generally permissive when present on actively replicating DNA templates and that this property of the HIV-1 promoter may be of importance to the activation of the DNA provirus in latently infected T cells. |
| first_indexed | 2024-03-07T07:00:56Z |
| format | Journal article |
| id | oxford-uuid:ffb8ca67-2746-4f6b-8ad3-2abd3d0e04e4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:00:56Z |
| publishDate | 1992 |
| record_format | dspace |
| spelling | oxford-uuid:ffb8ca67-2746-4f6b-8ad3-2abd3d0e04e42022-03-27T13:47:08ZMultiple SP1 binding sites confer enhancer-independent, replication-activated transcription of HIV-1 and globin gene promoters.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ffb8ca67-2746-4f6b-8ad3-2abd3d0e04e4EnglishSymplectic Elements at Oxford1992Proudfoot, NLee, BMonks, JWe demonstrate that multiple SP1 protein:DNA binding sites confer enhancer-independent activation on the HIV-1 and globin gene promoters. This activation process can be achieved either by DNA replication of the promoter-containing plasmid or by high concentrations of input plasmid DNA used in the transfections. In the case of HIV-1, the three SP1 sites adjacent to the promoters TATA box are essential for this activation process. Furthermore, the human beta globin gene, which is normally dependent on a linked enhancer for transcriptional activity, can be made enhancer independent by insertion of SP1 binding sites adjacent to its TATA box. We speculate that (SP1)n-TATA type RNA polymerase II promoters may be generally permissive when present on actively replicating DNA templates and that this property of the HIV-1 promoter may be of importance to the activation of the DNA provirus in latently infected T cells. |
| spellingShingle | Proudfoot, N Lee, B Monks, J Multiple SP1 binding sites confer enhancer-independent, replication-activated transcription of HIV-1 and globin gene promoters. |
| title | Multiple SP1 binding sites confer enhancer-independent, replication-activated transcription of HIV-1 and globin gene promoters. |
| title_full | Multiple SP1 binding sites confer enhancer-independent, replication-activated transcription of HIV-1 and globin gene promoters. |
| title_fullStr | Multiple SP1 binding sites confer enhancer-independent, replication-activated transcription of HIV-1 and globin gene promoters. |
| title_full_unstemmed | Multiple SP1 binding sites confer enhancer-independent, replication-activated transcription of HIV-1 and globin gene promoters. |
| title_short | Multiple SP1 binding sites confer enhancer-independent, replication-activated transcription of HIV-1 and globin gene promoters. |
| title_sort | multiple sp1 binding sites confer enhancer independent replication activated transcription of hiv 1 and globin gene promoters |
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