Optimisation of histamine reduction in kawakawa (Euthynnus Affinis) by red onion extract using Response Surface Methodology (RSM) / Nurul Dalila Nik Mat

The purpose of this study was to optimise the histamine reduction in kawakawa by red onion extract using Response Surface Methodology (RSM) of MINITAB Software (Version 14). Experimental design was created by RSM whereby test variables; amount of red onion extract (%), storage temperature (°C) and s...

Full description

Bibliographic Details
Main Author: Nik Mat, Nurul Dalila
Format: Student Project
Language:English
Published: Faculty of Applied Sciences 2009
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/22949/1/PPb_NURUL%20DALILA%20NIK%20MAT%20AS%2009_5.pdf
_version_ 1796901648824532992
author Nik Mat, Nurul Dalila
author_facet Nik Mat, Nurul Dalila
author_sort Nik Mat, Nurul Dalila
collection UITM
description The purpose of this study was to optimise the histamine reduction in kawakawa by red onion extract using Response Surface Methodology (RSM) of MINITAB Software (Version 14). Experimental design was created by RSM whereby test variables; amount of red onion extract (%), storage temperature (°C) and storage time (hours) were used. Half part of flesh kawakawa were blended and mixed thoroughly in different amounts of red onion extract and treated differently in terms of storage temperature and storage time as suggested by the experimental design of RSM. The histamine was extract by using n-butanol and 0.1M of HC1. Histamine analysis was carried out according to AOAC Flourometric Method, 1990 and measured using Flourosence at an excitation wavelength of 350 nm and emission wavelength of 444 nm. Histamine content in treated kawakawa was reduced by 75.85% at the optimum condition; 93.6% of red onion extract, -1.4 °C of storage temperature and 15.82 hours of storage time. In addition, the significant regression equations or model at the 5% level of confidence was also established for the estimation of the percentage reduction of histamine in kawakawa treated by red onion extract. This indicates that red onion extract is a potential source to be applied to reduce histamine in fish.
first_indexed 2024-03-06T01:53:14Z
format Student Project
id uitm.eprints-2949
institution Universiti Teknologi MARA
language English
last_indexed 2024-03-06T01:53:14Z
publishDate 2009
publisher Faculty of Applied Sciences
record_format dspace
spelling uitm.eprints-29492019-02-01T02:52:27Z https://ir.uitm.edu.my/id/eprint/22949/ Optimisation of histamine reduction in kawakawa (Euthynnus Affinis) by red onion extract using Response Surface Methodology (RSM) / Nurul Dalila Nik Mat Nik Mat, Nurul Dalila Neurophysiology and neuropsychology The purpose of this study was to optimise the histamine reduction in kawakawa by red onion extract using Response Surface Methodology (RSM) of MINITAB Software (Version 14). Experimental design was created by RSM whereby test variables; amount of red onion extract (%), storage temperature (°C) and storage time (hours) were used. Half part of flesh kawakawa were blended and mixed thoroughly in different amounts of red onion extract and treated differently in terms of storage temperature and storage time as suggested by the experimental design of RSM. The histamine was extract by using n-butanol and 0.1M of HC1. Histamine analysis was carried out according to AOAC Flourometric Method, 1990 and measured using Flourosence at an excitation wavelength of 350 nm and emission wavelength of 444 nm. Histamine content in treated kawakawa was reduced by 75.85% at the optimum condition; 93.6% of red onion extract, -1.4 °C of storage temperature and 15.82 hours of storage time. In addition, the significant regression equations or model at the 5% level of confidence was also established for the estimation of the percentage reduction of histamine in kawakawa treated by red onion extract. This indicates that red onion extract is a potential source to be applied to reduce histamine in fish. Faculty of Applied Sciences 2009 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/22949/1/PPb_NURUL%20DALILA%20NIK%20MAT%20AS%2009_5.pdf Optimisation of histamine reduction in kawakawa (Euthynnus Affinis) by red onion extract using Response Surface Methodology (RSM) / Nurul Dalila Nik Mat. (2009) [Student Project] <http://terminalib.uitm.edu.my/22949.pdf> (Unpublished)
spellingShingle Neurophysiology and neuropsychology
Nik Mat, Nurul Dalila
Optimisation of histamine reduction in kawakawa (Euthynnus Affinis) by red onion extract using Response Surface Methodology (RSM) / Nurul Dalila Nik Mat
title Optimisation of histamine reduction in kawakawa (Euthynnus Affinis) by red onion extract using Response Surface Methodology (RSM) / Nurul Dalila Nik Mat
title_full Optimisation of histamine reduction in kawakawa (Euthynnus Affinis) by red onion extract using Response Surface Methodology (RSM) / Nurul Dalila Nik Mat
title_fullStr Optimisation of histamine reduction in kawakawa (Euthynnus Affinis) by red onion extract using Response Surface Methodology (RSM) / Nurul Dalila Nik Mat
title_full_unstemmed Optimisation of histamine reduction in kawakawa (Euthynnus Affinis) by red onion extract using Response Surface Methodology (RSM) / Nurul Dalila Nik Mat
title_short Optimisation of histamine reduction in kawakawa (Euthynnus Affinis) by red onion extract using Response Surface Methodology (RSM) / Nurul Dalila Nik Mat
title_sort optimisation of histamine reduction in kawakawa euthynnus affinis by red onion extract using response surface methodology rsm nurul dalila nik mat
topic Neurophysiology and neuropsychology
url https://ir.uitm.edu.my/id/eprint/22949/1/PPb_NURUL%20DALILA%20NIK%20MAT%20AS%2009_5.pdf
work_keys_str_mv AT nikmatnuruldalila optimisationofhistaminereductioninkawakawaeuthynnusaffinisbyredonionextractusingresponsesurfacemethodologyrsmnuruldalilanikmat