Antagonistic activity endophytic bacteria isolate from oil palm roots against Ganoderma Sp. / Azerul Kamahudin

The oil palm (Elaeis guineensis) is commodity crop for Malaysia. In this study, an experiment was conducted to screening antagonistic activity of endophytic bacteria from oil palm roots. The aims was to identify bacteria that can be used to control Ganoderma sp. Root sample was taken from three diff...

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Main Author: Kamahudin, Azerul
Format: Student Project
Language:English
Published: Faculty of Plantation and Agrotechnology 2019
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/23867/1/23867.pdf
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author Kamahudin, Azerul
author_facet Kamahudin, Azerul
author_sort Kamahudin, Azerul
collection UITM
description The oil palm (Elaeis guineensis) is commodity crop for Malaysia. In this study, an experiment was conducted to screening antagonistic activity of endophytic bacteria from oil palm roots. The aims was to identify bacteria that can be used to control Ganoderma sp. Root sample was taken from three different place that is Segamat estate Johor, nursery Fong Shun trading Rompin and ladang uitm jasin, Melaka. For isolation of Ganoderma sp sample was being taken at private company research center at Gemencheh Melaka. A total of 30 roots sample were obtained with 10 sample from segamat estate Johor, 10 sample roots for in mature from nursery Fong Shun trading and 10 root samples form mature oil palm from ladang uitm Jasin. Bacteria that being isolates showed the antagonistic activity against Ganoderma sp by inhibition and slow down the growth rate of Ganoderma sp in culture plate. 27 endophytic bacteria were successfully isolated from roots sample and about 9 of it show the positive result, that slow down the growth of Ganoderma sp. Six out of 9 isolate were identified as Gram-negative bacteria and 3 were Gram-positive bacteria. Therefore, Gram-negative bacteria more in the oil palm roots compared to the Gram-positive bacteria. From 9 bacteria, only 2 bacteria that is success to suppress Ganoderma sp more the 50 % using the RIPG calculation method. These bacteria were identified and characterized. The bacteria colony form or shape is round and rod shape. Colony margin is entire and filamentous. Three type Colony elevation was identify that is raised, convex, and growth into medium.
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spelling uitm.eprints-38672020-08-05T05:54:29Z https://ir.uitm.edu.my/id/eprint/23867/ Antagonistic activity endophytic bacteria isolate from oil palm roots against Ganoderma Sp. / Azerul Kamahudin Kamahudin, Azerul Bacteria S Agriculture (General) SB Plant culture The oil palm (Elaeis guineensis) is commodity crop for Malaysia. In this study, an experiment was conducted to screening antagonistic activity of endophytic bacteria from oil palm roots. The aims was to identify bacteria that can be used to control Ganoderma sp. Root sample was taken from three different place that is Segamat estate Johor, nursery Fong Shun trading Rompin and ladang uitm jasin, Melaka. For isolation of Ganoderma sp sample was being taken at private company research center at Gemencheh Melaka. A total of 30 roots sample were obtained with 10 sample from segamat estate Johor, 10 sample roots for in mature from nursery Fong Shun trading and 10 root samples form mature oil palm from ladang uitm Jasin. Bacteria that being isolates showed the antagonistic activity against Ganoderma sp by inhibition and slow down the growth rate of Ganoderma sp in culture plate. 27 endophytic bacteria were successfully isolated from roots sample and about 9 of it show the positive result, that slow down the growth of Ganoderma sp. Six out of 9 isolate were identified as Gram-negative bacteria and 3 were Gram-positive bacteria. Therefore, Gram-negative bacteria more in the oil palm roots compared to the Gram-positive bacteria. From 9 bacteria, only 2 bacteria that is success to suppress Ganoderma sp more the 50 % using the RIPG calculation method. These bacteria were identified and characterized. The bacteria colony form or shape is round and rod shape. Colony margin is entire and filamentous. Three type Colony elevation was identify that is raised, convex, and growth into medium. Faculty of Plantation and Agrotechnology 2019 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/23867/1/23867.pdf Antagonistic activity endophytic bacteria isolate from oil palm roots against Ganoderma Sp. / Azerul Kamahudin. (2019) [Student Project] <http://terminalib.uitm.edu.my/23867.pdf> (Unpublished)
spellingShingle Bacteria
S Agriculture (General)
SB Plant culture
Kamahudin, Azerul
Antagonistic activity endophytic bacteria isolate from oil palm roots against Ganoderma Sp. / Azerul Kamahudin
title Antagonistic activity endophytic bacteria isolate from oil palm roots against Ganoderma Sp. / Azerul Kamahudin
title_full Antagonistic activity endophytic bacteria isolate from oil palm roots against Ganoderma Sp. / Azerul Kamahudin
title_fullStr Antagonistic activity endophytic bacteria isolate from oil palm roots against Ganoderma Sp. / Azerul Kamahudin
title_full_unstemmed Antagonistic activity endophytic bacteria isolate from oil palm roots against Ganoderma Sp. / Azerul Kamahudin
title_short Antagonistic activity endophytic bacteria isolate from oil palm roots against Ganoderma Sp. / Azerul Kamahudin
title_sort antagonistic activity endophytic bacteria isolate from oil palm roots against ganoderma sp azerul kamahudin
topic Bacteria
S Agriculture (General)
SB Plant culture
url https://ir.uitm.edu.my/id/eprint/23867/1/23867.pdf
work_keys_str_mv AT kamahudinazerul antagonisticactivityendophyticbacteriaisolatefromoilpalmrootsagainstganodermaspazerulkamahudin