Micropropagation and acclimatization of Musa Acuminata cv. Mas and Raja Udang / Syed Abdullah Syed Mustaffa

Tissue culture method is the best method to overcome the shortage of planting material. The plantlets produced by tissue culture method are identical to the parent plant. This research was aimed to produce high quality planting materials of Musa Acuminata cv. Mas and Raja Udang. It was also intended...

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Main Author: Syed Mustaffa, Syed Abdullah
Format: Student Project
Language:English
Published: Faculty of Plantation and Agrotechnology 2019
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/24484/1/24484.pdf
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author Syed Mustaffa, Syed Abdullah
author_facet Syed Mustaffa, Syed Abdullah
author_sort Syed Mustaffa, Syed Abdullah
collection UITM
description Tissue culture method is the best method to overcome the shortage of planting material. The plantlets produced by tissue culture method are identical to the parent plant. This research was aimed to produce high quality planting materials of Musa Acuminata cv. Mas and Raja Udang. It was also intended to compare the regeneration efficiency of the two cultivars studied. Murashige and Skoog media added with BAP and NAA hormones were prepared and used for micropropagation of the bananas. The banana suckers were collected and surface-sterilized with ethanol and sodium hypochlorite solution. Then, the explants were cultured on MS medium. The explants were cultured under different growth conditions (light or dark conditions) to investigate the effect of light on the regeneration efficiency of the explants. Evaluation was performed to compare the shoot regeneration efficiency of Musa acuminata cv. Mas and Raja Udang in tissue culture. The plantlets were transferred to the potting mix consisting of topsoil, sand and farm yard manure in the ratio of 1:1:1. Acclimatization of plantlets was carried out before field planting purpose.
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spelling uitm.eprints-44842020-08-11T08:57:47Z https://ir.uitm.edu.my/id/eprint/24484/ Micropropagation and acclimatization of Musa Acuminata cv. Mas and Raja Udang / Syed Abdullah Syed Mustaffa Syed Mustaffa, Syed Abdullah Cell culture Musaceae (Banana) Cultures and culture media. Culture technique. SB Plant culture Tissue culture method is the best method to overcome the shortage of planting material. The plantlets produced by tissue culture method are identical to the parent plant. This research was aimed to produce high quality planting materials of Musa Acuminata cv. Mas and Raja Udang. It was also intended to compare the regeneration efficiency of the two cultivars studied. Murashige and Skoog media added with BAP and NAA hormones were prepared and used for micropropagation of the bananas. The banana suckers were collected and surface-sterilized with ethanol and sodium hypochlorite solution. Then, the explants were cultured on MS medium. The explants were cultured under different growth conditions (light or dark conditions) to investigate the effect of light on the regeneration efficiency of the explants. Evaluation was performed to compare the shoot regeneration efficiency of Musa acuminata cv. Mas and Raja Udang in tissue culture. The plantlets were transferred to the potting mix consisting of topsoil, sand and farm yard manure in the ratio of 1:1:1. Acclimatization of plantlets was carried out before field planting purpose. Faculty of Plantation and Agrotechnology 2019 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/24484/1/24484.pdf Micropropagation and acclimatization of Musa Acuminata cv. Mas and Raja Udang / Syed Abdullah Syed Mustaffa. (2019) [Student Project] <http://terminalib.uitm.edu.my/24484.pdf> (Unpublished)
spellingShingle Cell culture
Musaceae (Banana)
Cultures and culture media. Culture technique.
SB Plant culture
Syed Mustaffa, Syed Abdullah
Micropropagation and acclimatization of Musa Acuminata cv. Mas and Raja Udang / Syed Abdullah Syed Mustaffa
title Micropropagation and acclimatization of Musa Acuminata cv. Mas and Raja Udang / Syed Abdullah Syed Mustaffa
title_full Micropropagation and acclimatization of Musa Acuminata cv. Mas and Raja Udang / Syed Abdullah Syed Mustaffa
title_fullStr Micropropagation and acclimatization of Musa Acuminata cv. Mas and Raja Udang / Syed Abdullah Syed Mustaffa
title_full_unstemmed Micropropagation and acclimatization of Musa Acuminata cv. Mas and Raja Udang / Syed Abdullah Syed Mustaffa
title_short Micropropagation and acclimatization of Musa Acuminata cv. Mas and Raja Udang / Syed Abdullah Syed Mustaffa
title_sort micropropagation and acclimatization of musa acuminata cv mas and raja udang syed abdullah syed mustaffa
topic Cell culture
Musaceae (Banana)
Cultures and culture media. Culture technique.
SB Plant culture
url https://ir.uitm.edu.my/id/eprint/24484/1/24484.pdf
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