Immobilization of lipase in calcium alginate beads for the study of butyl acetate synthesis / Mohd Zulkhairi Abdul Rahim
Literature about lipase (EC 3.1.1.3) , immobilized enzyme, alginate and chitosan are presented in thi s thesis. Thi s study involved the use of free and immobilized Iipases to catalyse esterification reaction of short chain fatty acid and alcohol to produce short chain ester. Generally , it involved...
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| Format: | Thesis |
| Language: | English |
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2006
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| Online Access: | https://ir.uitm.edu.my/id/eprint/27425/1/TM_MOHD%20ZULKHAIRI%20ABDUL%20RAHIM%20AS%2006_5.pdf |
| _version_ | 1796902500609032192 |
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| author | Abdul Rahim, Mohd Zulkhairi |
| author_facet | Abdul Rahim, Mohd Zulkhairi |
| author_sort | Abdul Rahim, Mohd Zulkhairi |
| collection | UITM |
| description | Literature about lipase (EC 3.1.1.3) , immobilized enzyme, alginate and chitosan are presented in thi s thesis. Thi s study involved the use of free and immobilized Iipases to catalyse esterification reaction of short chain fatty acid and alcohol to produce short chain ester. Generally , it involved the optimization of conditions of esterification reaction of acetic acid and n-butanol and followed by comparison of properties of immobilized enzyme with those of free enzyme. Results showed that 14.3 mg lipase , 80 umol n-butanol , 160 umol acetic acid and 3.0 days reaction time at a temp erature of 40 DC were the optimum conditions for lipase - CAB in terms of enzyme loading , immobilized enzyme concentration, temperature, substrate concentration and reaction time respectively. Meanwhile, 0.8% w/v of chitosan solution was chosen for the stabilized calcium alginate beads. Results showed that product conversion increased by increasing the temperature up to 50 DC for Lipase CAB and Lipase - CCAB but not for free lipase. Thermal stability test showed that Lipase - CAB and Lipa se - CCAB remained stable against temperature up to 60 DC compared to free lipase which had the highest activity at 30°C. The studies of effects of n-buta nol concentrations showed that increased in concentration of n-butanol above 40 umol decreased the conversion of product for Lipase - CCAB and free lipase. Meanwhile, conversion of product was affected by increasing concentration of n-butan ol to 80flmol and above for Lipase - CAB . In the study of effect of acetic acid, it was found that increasing concentration of acetic acid abovel60 umol decreased the product conver sion for Lipase - CAB and free lipase. However , Lipase - CCAB was not affected by high concentration of acetic acid up to 200 umol, Kinetic param eters , Km & Vmax of immobilized lipases for n-butanol were lower in values when compared with Km & Vmax values for acetic acid . Results showed that there were no stati stically significant different specific activities among the three systems studied. Operational stability test was important for repeated applications in batch or in a continuous reactor. It was demon strated that the enzyme was still active for at least 5 cycle s. Thus it was proven that immobilized lipase and free lipase were able to catalyse synthesis of short chain esters under the condition s studied. Continuous proce sses studies showed immobilized lipase had potential for such synthes is but need further studies . Several recommendations for further studies have also been suggested. |
| first_indexed | 2024-03-06T02:05:53Z |
| format | Thesis |
| id | uitm.eprints-7425 |
| institution | Universiti Teknologi MARA |
| language | English |
| last_indexed | 2024-03-06T02:05:53Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | uitm.eprints-74252020-01-20T02:56:29Z https://ir.uitm.edu.my/id/eprint/27425/ Immobilization of lipase in calcium alginate beads for the study of butyl acetate synthesis / Mohd Zulkhairi Abdul Rahim Abdul Rahim, Mohd Zulkhairi Biochemistry Literature about lipase (EC 3.1.1.3) , immobilized enzyme, alginate and chitosan are presented in thi s thesis. Thi s study involved the use of free and immobilized Iipases to catalyse esterification reaction of short chain fatty acid and alcohol to produce short chain ester. Generally , it involved the optimization of conditions of esterification reaction of acetic acid and n-butanol and followed by comparison of properties of immobilized enzyme with those of free enzyme. Results showed that 14.3 mg lipase , 80 umol n-butanol , 160 umol acetic acid and 3.0 days reaction time at a temp erature of 40 DC were the optimum conditions for lipase - CAB in terms of enzyme loading , immobilized enzyme concentration, temperature, substrate concentration and reaction time respectively. Meanwhile, 0.8% w/v of chitosan solution was chosen for the stabilized calcium alginate beads. Results showed that product conversion increased by increasing the temperature up to 50 DC for Lipase CAB and Lipase - CCAB but not for free lipase. Thermal stability test showed that Lipase - CAB and Lipa se - CCAB remained stable against temperature up to 60 DC compared to free lipase which had the highest activity at 30°C. The studies of effects of n-buta nol concentrations showed that increased in concentration of n-butanol above 40 umol decreased the conversion of product for Lipase - CCAB and free lipase. Meanwhile, conversion of product was affected by increasing concentration of n-butan ol to 80flmol and above for Lipase - CAB . In the study of effect of acetic acid, it was found that increasing concentration of acetic acid abovel60 umol decreased the product conver sion for Lipase - CAB and free lipase. However , Lipase - CCAB was not affected by high concentration of acetic acid up to 200 umol, Kinetic param eters , Km & Vmax of immobilized lipases for n-butanol were lower in values when compared with Km & Vmax values for acetic acid . Results showed that there were no stati stically significant different specific activities among the three systems studied. Operational stability test was important for repeated applications in batch or in a continuous reactor. It was demon strated that the enzyme was still active for at least 5 cycle s. Thus it was proven that immobilized lipase and free lipase were able to catalyse synthesis of short chain esters under the condition s studied. Continuous proce sses studies showed immobilized lipase had potential for such synthes is but need further studies . Several recommendations for further studies have also been suggested. 2006 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/27425/1/TM_MOHD%20ZULKHAIRI%20ABDUL%20RAHIM%20AS%2006_5.pdf Immobilization of lipase in calcium alginate beads for the study of butyl acetate synthesis / Mohd Zulkhairi Abdul Rahim. (2006) Masters thesis, thesis, Universiti Teknologi MARA. <http://terminalib.uitm.edu.my/27425.pdf> |
| spellingShingle | Biochemistry Abdul Rahim, Mohd Zulkhairi Immobilization of lipase in calcium alginate beads for the study of butyl acetate synthesis / Mohd Zulkhairi Abdul Rahim |
| title | Immobilization of lipase in calcium alginate beads for the study of butyl acetate synthesis / Mohd Zulkhairi Abdul Rahim |
| title_full | Immobilization of lipase in calcium alginate beads for the study of butyl acetate synthesis / Mohd Zulkhairi Abdul Rahim |
| title_fullStr | Immobilization of lipase in calcium alginate beads for the study of butyl acetate synthesis / Mohd Zulkhairi Abdul Rahim |
| title_full_unstemmed | Immobilization of lipase in calcium alginate beads for the study of butyl acetate synthesis / Mohd Zulkhairi Abdul Rahim |
| title_short | Immobilization of lipase in calcium alginate beads for the study of butyl acetate synthesis / Mohd Zulkhairi Abdul Rahim |
| title_sort | immobilization of lipase in calcium alginate beads for the study of butyl acetate synthesis mohd zulkhairi abdul rahim |
| topic | Biochemistry |
| url | https://ir.uitm.edu.my/id/eprint/27425/1/TM_MOHD%20ZULKHAIRI%20ABDUL%20RAHIM%20AS%2006_5.pdf |
| work_keys_str_mv | AT abdulrahimmohdzulkhairi immobilizationoflipaseincalciumalginatebeadsforthestudyofbutylacetatesynthesismohdzulkhairiabdulrahim |