Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria
Acetic acid bacteria (AAB) isolated from natural resources and fermented plant beverages were screened to produce 1,3-dihydroxyacetone (DHA) from non-detoxified crude glycerol. Among them, the isolate NKC115 was identified as Gluconobacter frateurii and produced the highest amounts of DHA. Subsequen...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2018
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| Online Access: | http://journalarticle.ukm.my/12029/1/07%20Varavut%20Tanamool.pdf |
| _version_ | 1796931602087936000 |
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| author | Varavut Tanamool, Piyorot Hongsachart, Wichai Soemphol, |
| author_facet | Varavut Tanamool, Piyorot Hongsachart, Wichai Soemphol, |
| author_sort | Varavut Tanamool, |
| collection | UKM |
| description | Acetic acid bacteria (AAB) isolated from natural resources and fermented plant beverages were screened to produce 1,3-dihydroxyacetone (DHA) from non-detoxified crude glycerol. Among them, the isolate NKC115 was identified as Gluconobacter frateurii and produced the highest amounts of DHA. Subsequently, the effects of growth-medium conditions (initial pH, crude glycerol concentration and nitrogen sources) on growth and DHA-production capability were examined. The results showed that the crude glycerol concentration increase to above 100 g/L suppressed growth and DHA production. The highest amount of DHA obtained was 27.50 g/L, from an initial crude glycerol concentration of 100 g/L. Meanwhile, an initial pH of 5.5-7.5 in the YPGc medium did not significantly affect the bacterial growth and DHA production. The optimal nitrogen source was peptone, with DHA production at 34.70 g/L. Furthermore, overexpression of the nhaK2 gene encoding for the Na+(K+)/H+ antiporter from Acetobactor tropicalis SKU1100 in G. frateurii NKC115 improved growth and increased the accumulation of DHA (37.25 g/L) from an initial crude glycerol concentration of 20%. These results indicated that the expression of this antiporter might maintain an optimal intracellular pH and concentration of Na+ or K+, leading to the cells’ ability to tolerate high concentrations of crude glycerol. |
| first_indexed | 2024-03-06T04:20:23Z |
| format | Article |
| id | ukm.eprints-12029 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:20:23Z |
| publishDate | 2018 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-120292018-09-09T23:15:19Z http://journalarticle.ukm.my/12029/ Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria Varavut Tanamool, Piyorot Hongsachart, Wichai Soemphol, Acetic acid bacteria (AAB) isolated from natural resources and fermented plant beverages were screened to produce 1,3-dihydroxyacetone (DHA) from non-detoxified crude glycerol. Among them, the isolate NKC115 was identified as Gluconobacter frateurii and produced the highest amounts of DHA. Subsequently, the effects of growth-medium conditions (initial pH, crude glycerol concentration and nitrogen sources) on growth and DHA-production capability were examined. The results showed that the crude glycerol concentration increase to above 100 g/L suppressed growth and DHA production. The highest amount of DHA obtained was 27.50 g/L, from an initial crude glycerol concentration of 100 g/L. Meanwhile, an initial pH of 5.5-7.5 in the YPGc medium did not significantly affect the bacterial growth and DHA production. The optimal nitrogen source was peptone, with DHA production at 34.70 g/L. Furthermore, overexpression of the nhaK2 gene encoding for the Na+(K+)/H+ antiporter from Acetobactor tropicalis SKU1100 in G. frateurii NKC115 improved growth and increased the accumulation of DHA (37.25 g/L) from an initial crude glycerol concentration of 20%. These results indicated that the expression of this antiporter might maintain an optimal intracellular pH and concentration of Na+ or K+, leading to the cells’ ability to tolerate high concentrations of crude glycerol. Penerbit Universiti Kebangsaan Malaysia 2018-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12029/1/07%20Varavut%20Tanamool.pdf Varavut Tanamool, and Piyorot Hongsachart, and Wichai Soemphol, (2018) Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria. Sains Malaysiana, 47 (3). pp. 481-488. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil3_2018/KandunganJilid47Bil3_2018.html |
| spellingShingle | Varavut Tanamool, Piyorot Hongsachart, Wichai Soemphol, Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria |
| title | Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria |
| title_full | Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria |
| title_fullStr | Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria |
| title_full_unstemmed | Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria |
| title_short | Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria |
| title_sort | bioconversion of biodiesel derived crude glycerol to 1 3 dihydroxyacetone by a potential acetic acid bacteria |
| url | http://journalarticle.ukm.my/12029/1/07%20Varavut%20Tanamool.pdf |
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