Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes
This study attempted to identify and determine distribution of Leptospira spp. in environmental samples using 16S rRNA and rpoβ genes amplification. The samples were collected from high risk areas in Selangor, Malaysia. A total of 105 environmental samples consisting of soil and water were subjected...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2018
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| Online Access: | http://journalarticle.ukm.my/12202/1/18%20Nurul%20Farhana%20Zulkifli.pdf |
| _version_ | 1796931634871664640 |
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| author | Nurul Farhana Zulkifli, Steffi Julan Wan, Vasantha Kumari Neela, Chee, Hui Yee Siti Norbaya Masri, Mazen M. Jamil Al-Obaidi, Mohd Nasir Mohd Desa, |
| author_facet | Nurul Farhana Zulkifli, Steffi Julan Wan, Vasantha Kumari Neela, Chee, Hui Yee Siti Norbaya Masri, Mazen M. Jamil Al-Obaidi, Mohd Nasir Mohd Desa, |
| author_sort | Nurul Farhana Zulkifli, |
| collection | UKM |
| description | This study attempted to identify and determine distribution of Leptospira spp. in environmental samples using 16S rRNA and rpoβ genes amplification. The samples were collected from high risk areas in Selangor, Malaysia. A total of 105 environmental samples consisting of soil and water were subjected to direct DNA extraction and PCR reaction. PCR products were analysed using gel electrophoresis and subjected to sequence analysis. Thirteen out of 105 (12.38%) samples were amplified for 16S rRNA with an expected amplicon size of 330 bp, while 50 out of 105 (47.62%) samples showed amplification using rpoβ primers, but were not of expected size. Of the 13 16S rRNA amplified samples, only 5 were identified as Leptospira in the gene sequence analysis and clustered under uncultured group via phylogenetic tree. This study showed the DNA-based approach using PCR and sequence analysis is able to detect the presence of Leptospira, although environmental samples may contain diverse microbial populations that may complicate the detection. Overall, the study suggested the importance of surveillance for Leptospira from environmental samples. |
| first_indexed | 2024-03-06T04:20:53Z |
| format | Article |
| id | ukm.eprints-12202 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:20:53Z |
| publishDate | 2018 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-122022018-10-16T13:29:20Z http://journalarticle.ukm.my/12202/ Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes Nurul Farhana Zulkifli, Steffi Julan Wan, Vasantha Kumari Neela, Chee, Hui Yee Siti Norbaya Masri, Mazen M. Jamil Al-Obaidi, Mohd Nasir Mohd Desa, This study attempted to identify and determine distribution of Leptospira spp. in environmental samples using 16S rRNA and rpoβ genes amplification. The samples were collected from high risk areas in Selangor, Malaysia. A total of 105 environmental samples consisting of soil and water were subjected to direct DNA extraction and PCR reaction. PCR products were analysed using gel electrophoresis and subjected to sequence analysis. Thirteen out of 105 (12.38%) samples were amplified for 16S rRNA with an expected amplicon size of 330 bp, while 50 out of 105 (47.62%) samples showed amplification using rpoβ primers, but were not of expected size. Of the 13 16S rRNA amplified samples, only 5 were identified as Leptospira in the gene sequence analysis and clustered under uncultured group via phylogenetic tree. This study showed the DNA-based approach using PCR and sequence analysis is able to detect the presence of Leptospira, although environmental samples may contain diverse microbial populations that may complicate the detection. Overall, the study suggested the importance of surveillance for Leptospira from environmental samples. Penerbit Universiti Kebangsaan Malaysia 2018-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12202/1/18%20Nurul%20Farhana%20Zulkifli.pdf Nurul Farhana Zulkifli, and Steffi Julan Wan, and Vasantha Kumari Neela, and Chee, Hui Yee and Siti Norbaya Masri, and Mazen M. Jamil Al-Obaidi, and Mohd Nasir Mohd Desa, (2018) Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes. Sains Malaysiana, 47 (8). pp. 1795-1800. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num8_2018/contentsVol47num8_2018.html |
| spellingShingle | Nurul Farhana Zulkifli, Steffi Julan Wan, Vasantha Kumari Neela, Chee, Hui Yee Siti Norbaya Masri, Mazen M. Jamil Al-Obaidi, Mohd Nasir Mohd Desa, Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes |
| title | Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes |
| title_full | Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes |
| title_fullStr | Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes |
| title_full_unstemmed | Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes |
| title_short | Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes |
| title_sort | detection of leptospira species in environmental samples by amplification of 16s rrna and rpoβ genes |
| url | http://journalarticle.ukm.my/12202/1/18%20Nurul%20Farhana%20Zulkifli.pdf |
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