Characterization and potential applications of a recombinant antifreeze protein from an antarctic yeast Glaciozyma antarctica produced in Pichia pastoris
Ice recrystallization during thawing post-cryopreservation results in extensive cellular damage and ultimately leads to cell death and reduced cell viability. Antifreeze proteins (AFPs) are a group of proteins that allow organisms to survive in subzero environments. These proteins have thermal hys...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2017
|
| Online Access: | http://journalarticle.ukm.my/12323/1/46_01_30.pdf |
| _version_ | 1796931656883372032 |
|---|---|
| author | Md Tab M., Hashim N.H.F., Abu Bakar F.D., Illias R., Najimudin N., Mahadi N.M., Murad A.M.A., |
| author_facet | Md Tab M., Hashim N.H.F., Abu Bakar F.D., Illias R., Najimudin N., Mahadi N.M., Murad A.M.A., |
| author_sort | Md Tab M., |
| collection | UKM |
| description | Ice recrystallization during thawing post-cryopreservation results in extensive cellular damage and ultimately leads to cell
death and reduced cell viability. Antifreeze proteins (AFPs) are a group of proteins that allow organisms to survive in subzero
environments. These proteins have thermal hysteresis and ice recrystallization inhibitory activities. In this present study,
we demonstrated the efficiency of a recombinant antifreeze protein from the Antarctic yeast, Glaciozyma antarctica, as a
recrystallization inhibitor (RI) of ice growth and assessed its application as a cryopreservative of the fungal cutinase enzyme
against freeze-thaw cycles. Recombinant Afp1 from G. antarctica, a psychrophilic yeast, has been produced in a methylotrophic
yeast, Pichia pastoris, system that results in the expression of a hyper-glycoprotein (~55 kDa). Recombinant Afp1 exhibits
antifreeze functions: thermal hysteresis (TH) and recrystallization inhibition where the highest TH values recorded for ~0.5°C
at 10 mg/mL. The cryoprotective effects of Afp1 on purified recombinant cutinase showed that Afp1 can retain enzymatic
activity up to ~20% when subjected to several cycles of freeze thawing. These findings indicate that Afp1 might act as a
cryoprotective agent and thus, has great potential in biotechnology applications. |
| first_indexed | 2024-03-06T04:21:13Z |
| format | Article |
| id | ukm.eprints-12323 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:21:13Z |
| publishDate | 2017 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-123232018-11-16T12:36:42Z http://journalarticle.ukm.my/12323/ Characterization and potential applications of a recombinant antifreeze protein from an antarctic yeast Glaciozyma antarctica produced in Pichia pastoris Md Tab M., Hashim N.H.F., Abu Bakar F.D., Illias R., Najimudin N., Mahadi N.M., Murad A.M.A., Ice recrystallization during thawing post-cryopreservation results in extensive cellular damage and ultimately leads to cell death and reduced cell viability. Antifreeze proteins (AFPs) are a group of proteins that allow organisms to survive in subzero environments. These proteins have thermal hysteresis and ice recrystallization inhibitory activities. In this present study, we demonstrated the efficiency of a recombinant antifreeze protein from the Antarctic yeast, Glaciozyma antarctica, as a recrystallization inhibitor (RI) of ice growth and assessed its application as a cryopreservative of the fungal cutinase enzyme against freeze-thaw cycles. Recombinant Afp1 from G. antarctica, a psychrophilic yeast, has been produced in a methylotrophic yeast, Pichia pastoris, system that results in the expression of a hyper-glycoprotein (~55 kDa). Recombinant Afp1 exhibits antifreeze functions: thermal hysteresis (TH) and recrystallization inhibition where the highest TH values recorded for ~0.5°C at 10 mg/mL. The cryoprotective effects of Afp1 on purified recombinant cutinase showed that Afp1 can retain enzymatic activity up to ~20% when subjected to several cycles of freeze thawing. These findings indicate that Afp1 might act as a cryoprotective agent and thus, has great potential in biotechnology applications. Penerbit Universiti Kebangsaan Malaysia 2017-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12323/1/46_01_30.pdf Md Tab M., and Hashim N.H.F., and Abu Bakar F.D., and Illias R., and Najimudin N., and Mahadi N.M., and Murad A.M.A., (2017) Characterization and potential applications of a recombinant antifreeze protein from an antarctic yeast Glaciozyma antarctica produced in Pichia pastoris. Malaysian Applied Biology, 46 (1). pp. 213-218. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=623&catid=59:current-view&Itemid=56 |
| spellingShingle | Md Tab M., Hashim N.H.F., Abu Bakar F.D., Illias R., Najimudin N., Mahadi N.M., Murad A.M.A., Characterization and potential applications of a recombinant antifreeze protein from an antarctic yeast Glaciozyma antarctica produced in Pichia pastoris |
| title | Characterization and potential applications of a
recombinant antifreeze protein from an antarctic
yeast Glaciozyma antarctica produced in Pichia pastoris |
| title_full | Characterization and potential applications of a
recombinant antifreeze protein from an antarctic
yeast Glaciozyma antarctica produced in Pichia pastoris |
| title_fullStr | Characterization and potential applications of a
recombinant antifreeze protein from an antarctic
yeast Glaciozyma antarctica produced in Pichia pastoris |
| title_full_unstemmed | Characterization and potential applications of a
recombinant antifreeze protein from an antarctic
yeast Glaciozyma antarctica produced in Pichia pastoris |
| title_short | Characterization and potential applications of a
recombinant antifreeze protein from an antarctic
yeast Glaciozyma antarctica produced in Pichia pastoris |
| title_sort | characterization and potential applications of a recombinant antifreeze protein from an antarctic yeast glaciozyma antarctica produced in pichia pastoris |
| url | http://journalarticle.ukm.my/12323/1/46_01_30.pdf |
| work_keys_str_mv | AT mdtabm characterizationandpotentialapplicationsofarecombinantantifreezeproteinfromanantarcticyeastglaciozymaantarcticaproducedinpichiapastoris AT hashimnhf characterizationandpotentialapplicationsofarecombinantantifreezeproteinfromanantarcticyeastglaciozymaantarcticaproducedinpichiapastoris AT abubakarfd characterizationandpotentialapplicationsofarecombinantantifreezeproteinfromanantarcticyeastglaciozymaantarcticaproducedinpichiapastoris AT illiasr characterizationandpotentialapplicationsofarecombinantantifreezeproteinfromanantarcticyeastglaciozymaantarcticaproducedinpichiapastoris AT najimudinn characterizationandpotentialapplicationsofarecombinantantifreezeproteinfromanantarcticyeastglaciozymaantarcticaproducedinpichiapastoris AT mahadinm characterizationandpotentialapplicationsofarecombinantantifreezeproteinfromanantarcticyeastglaciozymaantarcticaproducedinpichiapastoris AT muradama characterizationandpotentialapplicationsofarecombinantantifreezeproteinfromanantarcticyeastglaciozymaantarcticaproducedinpichiapastoris |