Menskala naik satu proses fermentasi berdasarkan pekali pemindahan isipadu (kLa)oksigen tetap
The production of the enzyme cytochrome P-450 by Saccharomyces cerevisiae N.C.Y.C. 754 in batch fermentations using a coconut water based medium was scaled-up from shake flask to a 2-liter stirred - tank fermenter scale using the method of constant volumetric transfer coefficient (kLa) of oxygen....
| Main Authors: | , , , |
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| Format: | Article |
| Published: |
1990
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| Summary: | The production of the enzyme cytochrome P-450 by Saccharomyces
cerevisiae N.C.Y.C. 754 in batch fermentations using a coconut water based medium
was scaled-up from shake flask to a 2-liter stirred - tank fermenter scale using the
method of constant volumetric transfer coefficient (kLa) of oxygen.. kLa values were
derived by fitting the mass transfer equation to the data of dissolved oxygen tension
(DOT) versus time on computer using the Simplex Method with two unknowns,
namely kLa and the electrode mass transfer coefficient (kap) of oxygen. The stirrer
speed (RPM) and the air flowrate, (A) in the 2-liter fermenter that produced the value
of kLa found in the optimised conditions in shake flask was approximated by trial and
error. A fermentation run in the 2-liter fermenter using these values of RPM and A
gave an enzyme yield comparable to that achieved in shake-flasks |
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