The protective effect of ettlingera coccinea (TUHAU) against autoxidation-induced ox brain homogenate
Oxidative stress involved in various pathological conditions. Plants have been proven to act as a natural exogenous antioxidant. The aim of this research is to investigate the protective effects of Etlingera coccinea leaves aqueous extract on autoxidation-induced ox brain homogenate. The brain homog...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2018
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| Online Access: | http://journalarticle.ukm.my/12938/1/23998-76976-1-PB.pdf |
| _version_ | 1796931774069080064 |
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| author | Nur Najmi Mohamad Anuar, Jamaludin Mohamed, Erni Norfardila Abu Hanipah, Nor Janna Yahya, Esther Mathias Ajik, Izatus Shima Taib, |
| author_facet | Nur Najmi Mohamad Anuar, Jamaludin Mohamed, Erni Norfardila Abu Hanipah, Nor Janna Yahya, Esther Mathias Ajik, Izatus Shima Taib, |
| author_sort | Nur Najmi Mohamad Anuar, |
| collection | UKM |
| description | Oxidative stress involved in various pathological conditions. Plants have been proven to act as a natural exogenous antioxidant. The aim of this research is to investigate the protective effects of Etlingera coccinea leaves aqueous extract on autoxidation-induced ox brain homogenate. The brain homogenate was divided into 7 groups: control group with PBS solution, positive control group with 100 μg/ml ascorbic acid, test group with 25, 50, 100, 200 and 400 μg/ml of E. coccinea. The antioxidant potential of E. coccinea aqueous extract has been evaluated by antioxidant capacity assay such as Total phenolic content (TPC), radical scavenging assay (DPPH) and ferric reducing antioxidant power (FRAP). Malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione (GSH) were also measured at 0 hr and 1 hr after 37°C water bath incubation to determine the antioxidant status and oxidative damage. TPC assay showed (4.85 ± 0.28) mg GAE/g of dry weight of E. coccinea leaves. Based on DPPH and FRAP assay, E. coccinea aqueous extract showed a dose-dependent antioxidant activity. MDA level in the 50 μg/ml E. coccinea was significantly lower compared to the other groups (p < 0.05). The SOD activity was significantly increase in 400 μg/ml E. coccinea (p < 0.05) compared to other groups. E. coccinea at the dose of 25 μg/ml and 50 μg/ml showed significant increase in GSH level compared to other groups (p < 0.05). In conclusion, 25 μg/ml and 50 μg/ml of E. coccinea leave aqueous extracts exhibited a potential protective effect on autoxidation-induced ox brain homogenate. |
| first_indexed | 2024-03-06T04:23:02Z |
| format | Article |
| id | ukm.eprints-12938 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:23:02Z |
| publishDate | 2018 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-129382019-05-17T14:35:09Z http://journalarticle.ukm.my/12938/ The protective effect of ettlingera coccinea (TUHAU) against autoxidation-induced ox brain homogenate Nur Najmi Mohamad Anuar, Jamaludin Mohamed, Erni Norfardila Abu Hanipah, Nor Janna Yahya, Esther Mathias Ajik, Izatus Shima Taib, Oxidative stress involved in various pathological conditions. Plants have been proven to act as a natural exogenous antioxidant. The aim of this research is to investigate the protective effects of Etlingera coccinea leaves aqueous extract on autoxidation-induced ox brain homogenate. The brain homogenate was divided into 7 groups: control group with PBS solution, positive control group with 100 μg/ml ascorbic acid, test group with 25, 50, 100, 200 and 400 μg/ml of E. coccinea. The antioxidant potential of E. coccinea aqueous extract has been evaluated by antioxidant capacity assay such as Total phenolic content (TPC), radical scavenging assay (DPPH) and ferric reducing antioxidant power (FRAP). Malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione (GSH) were also measured at 0 hr and 1 hr after 37°C water bath incubation to determine the antioxidant status and oxidative damage. TPC assay showed (4.85 ± 0.28) mg GAE/g of dry weight of E. coccinea leaves. Based on DPPH and FRAP assay, E. coccinea aqueous extract showed a dose-dependent antioxidant activity. MDA level in the 50 μg/ml E. coccinea was significantly lower compared to the other groups (p < 0.05). The SOD activity was significantly increase in 400 μg/ml E. coccinea (p < 0.05) compared to other groups. E. coccinea at the dose of 25 μg/ml and 50 μg/ml showed significant increase in GSH level compared to other groups (p < 0.05). In conclusion, 25 μg/ml and 50 μg/ml of E. coccinea leave aqueous extracts exhibited a potential protective effect on autoxidation-induced ox brain homogenate. Penerbit Universiti Kebangsaan Malaysia 2018 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12938/1/23998-76976-1-PB.pdf Nur Najmi Mohamad Anuar, and Jamaludin Mohamed, and Erni Norfardila Abu Hanipah, and Nor Janna Yahya, and Esther Mathias Ajik, and Izatus Shima Taib, (2018) The protective effect of ettlingera coccinea (TUHAU) against autoxidation-induced ox brain homogenate. Jurnal Sains Kesihatan Malaysia, 16 (SI). pp. 35-39. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/1072 |
| spellingShingle | Nur Najmi Mohamad Anuar, Jamaludin Mohamed, Erni Norfardila Abu Hanipah, Nor Janna Yahya, Esther Mathias Ajik, Izatus Shima Taib, The protective effect of ettlingera coccinea (TUHAU) against autoxidation-induced ox brain homogenate |
| title | The protective effect of ettlingera coccinea (TUHAU) against autoxidation-induced ox brain homogenate |
| title_full | The protective effect of ettlingera coccinea (TUHAU) against autoxidation-induced ox brain homogenate |
| title_fullStr | The protective effect of ettlingera coccinea (TUHAU) against autoxidation-induced ox brain homogenate |
| title_full_unstemmed | The protective effect of ettlingera coccinea (TUHAU) against autoxidation-induced ox brain homogenate |
| title_short | The protective effect of ettlingera coccinea (TUHAU) against autoxidation-induced ox brain homogenate |
| title_sort | protective effect of ettlingera coccinea tuhau against autoxidation induced ox brain homogenate |
| url | http://journalarticle.ukm.my/12938/1/23998-76976-1-PB.pdf |
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