Characterization of the antimicrobial substances produced by Nibribacter radioresistens
This study characterized the antimicrobial substances produced by the radiation-resistant bacterium Nibribacter radioresistens. The antimicrobial substances showed activity against Salmonella Gallinarum, pathogenic Escherichia coli, Bacillus cereus, Streptococcus iniae, and Saccharomyces cerevisiae....
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://journalarticle.ukm.my/14375/1/08%20Sam%20Woong%20Kim.pdf |
| _version_ | 1796932057259048960 |
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| author | Kim, Sam Woong Ha, Yeon Jo Gal, Sang Wan Lee, Kyu Pil Bang, Kyu Ho Kang, Myung-Suk Yeo, Joo-Hong Yang, Hee-Sun Jeon, Seung-Ho Bang, Woo Young |
| author_facet | Kim, Sam Woong Ha, Yeon Jo Gal, Sang Wan Lee, Kyu Pil Bang, Kyu Ho Kang, Myung-Suk Yeo, Joo-Hong Yang, Hee-Sun Jeon, Seung-Ho Bang, Woo Young |
| author_sort | Kim, Sam Woong |
| collection | UKM |
| description | This study characterized the antimicrobial substances produced by the radiation-resistant bacterium Nibribacter radioresistens. The antimicrobial substances showed activity against Salmonella Gallinarum, pathogenic Escherichia coli, Bacillus cereus, Streptococcus iniae, and Saccharomyces cerevisiae. The substances showed higher activity against Gram-positive bacteria than against Gram-negative bacteria and yeast. N. radioresistens showed the best growth rate in LB liquid medium at 37ºC; however, production of the antimicrobial substances was not associated with growth. Since the activity of the antimicrobial substances was affected by proteinase K and EDTA, the substances were presumed to be antimicrobial peptides (AMPs). The antimicrobial substances produced by N. radioresistens were unstable at higher temperatures and in acidic and basic pH ranges, and most of the activity was attributed to either low (<3 kDa) or high molecular weight (>30 kDa) molecules. When S. Gallinarum was treated with the antimicrobial substances, the cell destruction was acted on the cell envelope. Therefore, we concluded that N. radioresistens produces broad-spectrum and very unstable antimicrobial substances that mostly consist of low- and high-molecular weight peptides. |
| first_indexed | 2024-03-06T04:27:13Z |
| format | Article |
| id | ukm.eprints-14375 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:27:13Z |
| publishDate | 2019 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-143752020-03-10T02:25:30Z http://journalarticle.ukm.my/14375/ Characterization of the antimicrobial substances produced by Nibribacter radioresistens Kim, Sam Woong Ha, Yeon Jo Gal, Sang Wan Lee, Kyu Pil Bang, Kyu Ho Kang, Myung-Suk Yeo, Joo-Hong Yang, Hee-Sun Jeon, Seung-Ho Bang, Woo Young This study characterized the antimicrobial substances produced by the radiation-resistant bacterium Nibribacter radioresistens. The antimicrobial substances showed activity against Salmonella Gallinarum, pathogenic Escherichia coli, Bacillus cereus, Streptococcus iniae, and Saccharomyces cerevisiae. The substances showed higher activity against Gram-positive bacteria than against Gram-negative bacteria and yeast. N. radioresistens showed the best growth rate in LB liquid medium at 37ºC; however, production of the antimicrobial substances was not associated with growth. Since the activity of the antimicrobial substances was affected by proteinase K and EDTA, the substances were presumed to be antimicrobial peptides (AMPs). The antimicrobial substances produced by N. radioresistens were unstable at higher temperatures and in acidic and basic pH ranges, and most of the activity was attributed to either low (<3 kDa) or high molecular weight (>30 kDa) molecules. When S. Gallinarum was treated with the antimicrobial substances, the cell destruction was acted on the cell envelope. Therefore, we concluded that N. radioresistens produces broad-spectrum and very unstable antimicrobial substances that mostly consist of low- and high-molecular weight peptides. Penerbit Universiti Kebangsaan Malaysia 2019-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/14375/1/08%20Sam%20Woong%20Kim.pdf Kim, Sam Woong and Ha, Yeon Jo and Gal, Sang Wan and Lee, Kyu Pil and Bang, Kyu Ho and Kang, Myung-Suk and Yeo, Joo-Hong and Yang, Hee-Sun and Jeon, Seung-Ho and Bang, Woo Young (2019) Characterization of the antimicrobial substances produced by Nibribacter radioresistens. Sains Malaysiana, 48 (10). pp. 2135-2141. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil10_2019/KandunganJilid48Bil10_2019.htm |
| spellingShingle | Kim, Sam Woong Ha, Yeon Jo Gal, Sang Wan Lee, Kyu Pil Bang, Kyu Ho Kang, Myung-Suk Yeo, Joo-Hong Yang, Hee-Sun Jeon, Seung-Ho Bang, Woo Young Characterization of the antimicrobial substances produced by Nibribacter radioresistens |
| title | Characterization of the antimicrobial substances produced
by Nibribacter radioresistens |
| title_full | Characterization of the antimicrobial substances produced
by Nibribacter radioresistens |
| title_fullStr | Characterization of the antimicrobial substances produced
by Nibribacter radioresistens |
| title_full_unstemmed | Characterization of the antimicrobial substances produced
by Nibribacter radioresistens |
| title_short | Characterization of the antimicrobial substances produced
by Nibribacter radioresistens |
| title_sort | characterization of the antimicrobial substances produced by nibribacter radioresistens |
| url | http://journalarticle.ukm.my/14375/1/08%20Sam%20Woong%20Kim.pdf |
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