Detection of Porcine DNA in gelatin containing muffins using Porcine Gene ChipTM analysis
This study was carried out to compare the sensitivity of two detection techniques using conventional polymerase chain reaction (PCR) and Porcine gene chip (OliproTM, Selangor, MY). Experimental samples consist of 15 muffins added with different percentage of porcine gelatin (1, 0.5, 0.1, 0.05, 0.0...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://journalarticle.ukm.my/14676/1/48_02_18.pdf |
| _version_ | 1796932118823043072 |
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| author | Nursheila Mustafa Muin, Sahilah Abd. Mutalib, Aishah Elias, Norrakiah Abdullah Sani, Aminah Abdullah, Razalee Sedek, Izhar Ariff Mohd. Kashim, |
| author_facet | Nursheila Mustafa Muin, Sahilah Abd. Mutalib, Aishah Elias, Norrakiah Abdullah Sani, Aminah Abdullah, Razalee Sedek, Izhar Ariff Mohd. Kashim, |
| author_sort | Nursheila Mustafa Muin, |
| collection | UKM |
| description | This study was carried out to compare the sensitivity of two detection techniques using conventional polymerase chain reaction
(PCR) and Porcine gene chip (OliproTM, Selangor, MY). Experimental samples consist of 15 muffins added with different
percentage of porcine gelatin (1, 0.5, 0.1, 0.05, 0.01 and 0% w/w), and then cooked in two different ways (steamed and
baked) were analyzed for the presence of porcine DNA in gelatin. Both Porcine gene chip and PCR analysis were targeting
the gene of cytochrome b (cyt b). Porcine DNA detection in muffin samples using gene chip and PCR method were able to
detect the presence of the porcine DNA at 73 and 53% of samples, respectively. Thus, this study demonstrated that the
sensitivity level of porcine the DNA detection was high in Porcine Gene chip in comparisons with the PCR analysis. |
| first_indexed | 2024-03-06T04:28:04Z |
| format | Article |
| id | ukm.eprints-14676 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:28:04Z |
| publishDate | 2019 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-146762020-05-27T01:10:52Z http://journalarticle.ukm.my/14676/ Detection of Porcine DNA in gelatin containing muffins using Porcine Gene ChipTM analysis Nursheila Mustafa Muin, Sahilah Abd. Mutalib, Aishah Elias, Norrakiah Abdullah Sani, Aminah Abdullah, Razalee Sedek, Izhar Ariff Mohd. Kashim, This study was carried out to compare the sensitivity of two detection techniques using conventional polymerase chain reaction (PCR) and Porcine gene chip (OliproTM, Selangor, MY). Experimental samples consist of 15 muffins added with different percentage of porcine gelatin (1, 0.5, 0.1, 0.05, 0.01 and 0% w/w), and then cooked in two different ways (steamed and baked) were analyzed for the presence of porcine DNA in gelatin. Both Porcine gene chip and PCR analysis were targeting the gene of cytochrome b (cyt b). Porcine DNA detection in muffin samples using gene chip and PCR method were able to detect the presence of the porcine DNA at 73 and 53% of samples, respectively. Thus, this study demonstrated that the sensitivity level of porcine the DNA detection was high in Porcine Gene chip in comparisons with the PCR analysis. Penerbit Universiti Kebangsaan Malaysia 2019-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/14676/1/48_02_18.pdf Nursheila Mustafa Muin, and Sahilah Abd. Mutalib, and Aishah Elias, and Norrakiah Abdullah Sani, and Aminah Abdullah, and Razalee Sedek, and Izhar Ariff Mohd. Kashim, (2019) Detection of Porcine DNA in gelatin containing muffins using Porcine Gene ChipTM analysis. Malaysian Applied Biology, 48 (2). pp. 125-130. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=921&catid=59:current-view&Itemid=56 |
| spellingShingle | Nursheila Mustafa Muin, Sahilah Abd. Mutalib, Aishah Elias, Norrakiah Abdullah Sani, Aminah Abdullah, Razalee Sedek, Izhar Ariff Mohd. Kashim, Detection of Porcine DNA in gelatin containing muffins using Porcine Gene ChipTM analysis |
| title | Detection of Porcine DNA in gelatin containing muffins using Porcine Gene ChipTM analysis |
| title_full | Detection of Porcine DNA in gelatin containing muffins using Porcine Gene ChipTM analysis |
| title_fullStr | Detection of Porcine DNA in gelatin containing muffins using Porcine Gene ChipTM analysis |
| title_full_unstemmed | Detection of Porcine DNA in gelatin containing muffins using Porcine Gene ChipTM analysis |
| title_short | Detection of Porcine DNA in gelatin containing muffins using Porcine Gene ChipTM analysis |
| title_sort | detection of porcine dna in gelatin containing muffins using porcine gene chiptm analysis |
| url | http://journalarticle.ukm.my/14676/1/48_02_18.pdf |
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