Anti-inflammatory effect of tamarind seed coat extract against LPS-induced RAW264.7 macrophages
Inflammatory response is modulated by stimulated immune cells, and has a pivotal role in host defense system against various stimuli. In this study, we evaluated the anti-inflammatory property of tamarind seed coat extract (TSCE) in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. Various co...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/15465/1/8.pdf |
| _version_ | 1796932262282919936 |
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| author | Jirapa Puntarut, Wipawadee Sianglum, Supita Tanasawet, Pennapa Chonpathompikunlert, Wanida Sukketsiri, |
| author_facet | Jirapa Puntarut, Wipawadee Sianglum, Supita Tanasawet, Pennapa Chonpathompikunlert, Wanida Sukketsiri, |
| author_sort | Jirapa Puntarut, |
| collection | UKM |
| description | Inflammatory response is modulated by stimulated immune cells, and has a pivotal role in host defense system against
various stimuli. In this study, we evaluated the anti-inflammatory property of tamarind seed coat extract (TSCE) in
lipopolysaccharide (LPS)-induced RAW264.7 macrophages. Various concentrations of TSCE (10, 25, and 50 µg mL-1)
were applied and then stimulated with LPS (1 μg mL-1) in RAW264.7 macrophages and the level of reactive oxygen
species (ROS) and nitric oxide (NO) were measured. Besides, enzyme-linked immunosorbent assay (ELISA) was used to
measure the level of pro-inflammatory cytokines. Our results showed that TSCE suppressed LPS-induced intracellular
ROS production and suppressed the NO levels in a dose-dependent manner. Significantly, the anti-inflammatory activity
was correlated with a lowered LPS-stimulated TNF-α and IL-1β pro-inflammatory cytokines. These results implied
that TSCE possess potent anti-inflammatory activity, which supported new insights into the TSCE utilization to protect
inflammation-related disorders. |
| first_indexed | 2024-03-06T04:30:10Z |
| format | Article |
| id | ukm.eprints-15465 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:30:10Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-154652020-10-30T04:25:55Z http://journalarticle.ukm.my/15465/ Anti-inflammatory effect of tamarind seed coat extract against LPS-induced RAW264.7 macrophages Jirapa Puntarut, Wipawadee Sianglum, Supita Tanasawet, Pennapa Chonpathompikunlert, Wanida Sukketsiri, Inflammatory response is modulated by stimulated immune cells, and has a pivotal role in host defense system against various stimuli. In this study, we evaluated the anti-inflammatory property of tamarind seed coat extract (TSCE) in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. Various concentrations of TSCE (10, 25, and 50 µg mL-1) were applied and then stimulated with LPS (1 μg mL-1) in RAW264.7 macrophages and the level of reactive oxygen species (ROS) and nitric oxide (NO) were measured. Besides, enzyme-linked immunosorbent assay (ELISA) was used to measure the level of pro-inflammatory cytokines. Our results showed that TSCE suppressed LPS-induced intracellular ROS production and suppressed the NO levels in a dose-dependent manner. Significantly, the anti-inflammatory activity was correlated with a lowered LPS-stimulated TNF-α and IL-1β pro-inflammatory cytokines. These results implied that TSCE possess potent anti-inflammatory activity, which supported new insights into the TSCE utilization to protect inflammation-related disorders. Penerbit Universiti Kebangsaan Malaysia 2020-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/15465/1/8.pdf Jirapa Puntarut, and Wipawadee Sianglum, and Supita Tanasawet, and Pennapa Chonpathompikunlert, and Wanida Sukketsiri, (2020) Anti-inflammatory effect of tamarind seed coat extract against LPS-induced RAW264.7 macrophages. Sains Malaysiana, 49 (6). pp. 1303-1312. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid49bil6_2020/KandunganJilid49Bil6_2020.html |
| spellingShingle | Jirapa Puntarut, Wipawadee Sianglum, Supita Tanasawet, Pennapa Chonpathompikunlert, Wanida Sukketsiri, Anti-inflammatory effect of tamarind seed coat extract against LPS-induced RAW264.7 macrophages |
| title | Anti-inflammatory effect of tamarind seed coat extract against LPS-induced RAW264.7 macrophages |
| title_full | Anti-inflammatory effect of tamarind seed coat extract against LPS-induced RAW264.7 macrophages |
| title_fullStr | Anti-inflammatory effect of tamarind seed coat extract against LPS-induced RAW264.7 macrophages |
| title_full_unstemmed | Anti-inflammatory effect of tamarind seed coat extract against LPS-induced RAW264.7 macrophages |
| title_short | Anti-inflammatory effect of tamarind seed coat extract against LPS-induced RAW264.7 macrophages |
| title_sort | anti inflammatory effect of tamarind seed coat extract against lps induced raw264 7 macrophages |
| url | http://journalarticle.ukm.my/15465/1/8.pdf |
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