Summary: | Androgen ablation therapy using gonadotropin-releasing hormone agonists is
reported to be associated with metabolic abnormalities. Annatto tocotrienol
(AnTT) is reported to reduce the expression of genes related to adipogenesis but the
mechanism remains elusive. This study sought to determine the effects of annatto
tocotrienol on body composition (lean and fat mass), serum adiponectin, leptin,
and glucose levels in male rats treated with buserelin, a testosterone ablation agent.
Three-month-old male Sprague Dawley rats (n=32) were randomly divided into
four groups. The normal control (n=8) was given corn oil orally daily and normal
saline subcutaneously daily. The remaining groups were injected with buserelin
subcutaneously (75µg/kg/day). The buserelin group (n=8) was given corn oil orally,
while the treatment groups were supplemented orally with AnTT at 60 or 100 mg/
kg (n = 8/group). After treatment of 12 weeks, rats were euthanized. Dual-energy
x-ray absorptiometry was performed to determine the lean and fat mass of the
rats. Blood was collected for the evaluation of adiponectin, leptin and glucose
levels. After 12 weeks, the lean mass, fat mass, adiponectin and leptin levels for
all groups increased significantly compared to their respective baseline levels
irrespective of their treatment (P<0.05). All groups, except rats receiving AnTT at 60
mg/kg, experienced a significant increase in glucose level after 3 months (P<0.05).
Androgen ablation and AnTT do not influence body composition, adiponectin and
leptin levels in male rats. However, annatto tocotrienol at 60 mg/kg may improve
glucose metabolism.
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