Acyl homoserine lactone lactonase bacteria potential as biocontrol agent of soft rot infection
The virulence gene expression of phytopathogenic bacteria, such as Dickeya dadantii is regulated by quorum sensing (QS) mechanism. The QS mechanism is induced by acyl homoserine lactone (AHL) that is universal autoinducer of Gram-negative bacteria. Degradation of AHL compound can be used to contro...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/15708/1/49_01_15.pdf |
| _version_ | 1796932304379052032 |
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| author | Erma Suryanti, Wahyudi, Aris Tri Akhdiya, Alina Rusmana, Iman |
| author_facet | Erma Suryanti, Wahyudi, Aris Tri Akhdiya, Alina Rusmana, Iman |
| author_sort | Erma Suryanti, |
| collection | UKM |
| description | The virulence gene expression of phytopathogenic bacteria, such as Dickeya dadantii is regulated by quorum sensing (QS)
mechanism. The QS mechanism is induced by acyl homoserine lactone (AHL) that is universal autoinducer of Gram-negative
bacteria. Degradation of AHL compound can be used to control the virulence of phytopathogenic bacteria. The enzyme of
AHL lactonase is encoded by the aiiA gene and can degrade the lactone ring of AHL. This study was aimed to characterize
aiiA gene encoding AHL lactonase from 20 isolates of shrimp pond and soil forest, Indonesia and examine the isolates
ability to reduce violacein pigment of Chromobacterium violaceum and soft rot disease symptoms caused by D. dadantii.
The results showed nine bacterial isolates (B4, B10, B11, B13, B14, B16, B17, B18 and B19) containing the aiiA gene
encoding AHL lactonase. Based on 16S rRNA gene identification, 9 isolates had the highest similarity with Bacillus toyonensis,
B. cereus, B. thuringiensis, B. mycoides and B. subtilis. These isolates had the capability to reduce the formation of violacein
pigment and the symptoms of soft rot disease in potatoes. These results show that the AHL lactonase produced by these
isolates can be used as Quorum Quenching (QQ) agent to inhibit the QS mechanism |
| first_indexed | 2024-03-06T04:30:49Z |
| format | Article |
| id | ukm.eprints-15708 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:30:49Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-157082020-11-18T06:03:58Z http://journalarticle.ukm.my/15708/ Acyl homoserine lactone lactonase bacteria potential as biocontrol agent of soft rot infection Erma Suryanti, Wahyudi, Aris Tri Akhdiya, Alina Rusmana, Iman The virulence gene expression of phytopathogenic bacteria, such as Dickeya dadantii is regulated by quorum sensing (QS) mechanism. The QS mechanism is induced by acyl homoserine lactone (AHL) that is universal autoinducer of Gram-negative bacteria. Degradation of AHL compound can be used to control the virulence of phytopathogenic bacteria. The enzyme of AHL lactonase is encoded by the aiiA gene and can degrade the lactone ring of AHL. This study was aimed to characterize aiiA gene encoding AHL lactonase from 20 isolates of shrimp pond and soil forest, Indonesia and examine the isolates ability to reduce violacein pigment of Chromobacterium violaceum and soft rot disease symptoms caused by D. dadantii. The results showed nine bacterial isolates (B4, B10, B11, B13, B14, B16, B17, B18 and B19) containing the aiiA gene encoding AHL lactonase. Based on 16S rRNA gene identification, 9 isolates had the highest similarity with Bacillus toyonensis, B. cereus, B. thuringiensis, B. mycoides and B. subtilis. These isolates had the capability to reduce the formation of violacein pigment and the symptoms of soft rot disease in potatoes. These results show that the AHL lactonase produced by these isolates can be used as Quorum Quenching (QQ) agent to inhibit the QS mechanism Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/15708/1/49_01_15.pdf Erma Suryanti, and Wahyudi, Aris Tri and Akhdiya, Alina and Rusmana, Iman (2020) Acyl homoserine lactone lactonase bacteria potential as biocontrol agent of soft rot infection. Malaysian Applied Biology, 49 (1). pp. 131-140. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=981&catid=59:current-view&Itemid=56 |
| spellingShingle | Erma Suryanti, Wahyudi, Aris Tri Akhdiya, Alina Rusmana, Iman Acyl homoserine lactone lactonase bacteria potential as biocontrol agent of soft rot infection |
| title | Acyl homoserine lactone lactonase bacteria potential as biocontrol agent of soft rot infection |
| title_full | Acyl homoserine lactone lactonase bacteria potential as biocontrol agent of soft rot infection |
| title_fullStr | Acyl homoserine lactone lactonase bacteria potential as biocontrol agent of soft rot infection |
| title_full_unstemmed | Acyl homoserine lactone lactonase bacteria potential as biocontrol agent of soft rot infection |
| title_short | Acyl homoserine lactone lactonase bacteria potential as biocontrol agent of soft rot infection |
| title_sort | acyl homoserine lactone lactonase bacteria potential as biocontrol agent of soft rot infection |
| url | http://journalarticle.ukm.my/15708/1/49_01_15.pdf |
| work_keys_str_mv | AT ermasuryanti acylhomoserinelactonelactonasebacteriapotentialasbiocontrolagentofsoftrotinfection AT wahyudiaristri acylhomoserinelactonelactonasebacteriapotentialasbiocontrolagentofsoftrotinfection AT akhdiyaalina acylhomoserinelactonelactonasebacteriapotentialasbiocontrolagentofsoftrotinfection AT rusmanaiman acylhomoserinelactonelactonasebacteriapotentialasbiocontrolagentofsoftrotinfection |