High-dose edible bird’s nest extract (EBN) upregulates LDL-R via suppression of HMGCR gene expression in HepG2 cell lines
Edible bird’s nest (EBN) is an ancient food that had been consumed by Chinese people for well-being purposes. Nevertheless, the effect of EBN on cholesterol metabolism is poorly studied. Since the cholesterol is primarily being metabolized in the liver, HepG2 cell lines was selected as the model f...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2020
|
| Online Access: | http://journalarticle.ukm.my/15939/1/9.pdf |
| _version_ | 1796932373877620736 |
|---|---|
| author | Mohd Noor Akmal, Intan-Shameha Abdul Razak, Rozaihan Mansor, Aini Ideris, Abdul Rahman Omar, Jalila Abu, Mokrish Ajat, |
| author_facet | Mohd Noor Akmal, Intan-Shameha Abdul Razak, Rozaihan Mansor, Aini Ideris, Abdul Rahman Omar, Jalila Abu, Mokrish Ajat, |
| author_sort | Mohd Noor Akmal, |
| collection | UKM |
| description | Edible bird’s nest (EBN) is an ancient food that had been consumed by Chinese people for well-being purposes.
Nevertheless, the effect of EBN on cholesterol metabolism is poorly studied. Since the cholesterol is primarily being
metabolized in the liver, HepG2 cell lines was selected as the model for in-vitro preliminary assessment. Our EBN
extract (EBNE) showed 71% cell viability in HepG2 cell lines, even at the highest dose (1.5 mg/mL). Simultaneously, EBNE
was significantly up-regulated the LDL-R gene expression via suppression of HMGCR at the highest concentration.
Besides that, ACAT2 was up-regulated significantly to allow exogenous cholesterol storage. Expression analysis of
these genes were correlated with high immunofluorescence distribution of the LDL-R and LDs compared to other
treatment groups. Consistent with gene expression and immunofluorescence staining, intrahepatocellular cholesterol
concentration was increased significantly at the highest dose of EBNE. The EBNE had significantly improved
cholesterol metabolism in the HepG2 cell line via suppression of the HMGCR gene and subsequently up-regulated
LDL-R gene expression at the highest dose. Therefore, this study provides an insight to understand the effect of EBNE
in improving cholesterol metabolism. |
| first_indexed | 2024-03-06T04:31:28Z |
| format | Article |
| id | ukm.eprints-15939 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:31:28Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-159392020-12-10T20:24:25Z http://journalarticle.ukm.my/15939/ High-dose edible bird’s nest extract (EBN) upregulates LDL-R via suppression of HMGCR gene expression in HepG2 cell lines Mohd Noor Akmal, Intan-Shameha Abdul Razak, Rozaihan Mansor, Aini Ideris, Abdul Rahman Omar, Jalila Abu, Mokrish Ajat, Edible bird’s nest (EBN) is an ancient food that had been consumed by Chinese people for well-being purposes. Nevertheless, the effect of EBN on cholesterol metabolism is poorly studied. Since the cholesterol is primarily being metabolized in the liver, HepG2 cell lines was selected as the model for in-vitro preliminary assessment. Our EBN extract (EBNE) showed 71% cell viability in HepG2 cell lines, even at the highest dose (1.5 mg/mL). Simultaneously, EBNE was significantly up-regulated the LDL-R gene expression via suppression of HMGCR at the highest concentration. Besides that, ACAT2 was up-regulated significantly to allow exogenous cholesterol storage. Expression analysis of these genes were correlated with high immunofluorescence distribution of the LDL-R and LDs compared to other treatment groups. Consistent with gene expression and immunofluorescence staining, intrahepatocellular cholesterol concentration was increased significantly at the highest dose of EBNE. The EBNE had significantly improved cholesterol metabolism in the HepG2 cell line via suppression of the HMGCR gene and subsequently up-regulated LDL-R gene expression at the highest dose. Therefore, this study provides an insight to understand the effect of EBNE in improving cholesterol metabolism. Penerbit Universiti Kebangsaan Malaysia 2020-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/15939/1/9.pdf Mohd Noor Akmal, and Intan-Shameha Abdul Razak, and Rozaihan Mansor, and Aini Ideris, and Abdul Rahman Omar, and Jalila Abu, and Mokrish Ajat, (2020) High-dose edible bird’s nest extract (EBN) upregulates LDL-R via suppression of HMGCR gene expression in HepG2 cell lines. Sains Malaysiana, 49 (10). pp. 2433-2442. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid49bil10_2020/KandunganJilid49Bil10_2020.html |
| spellingShingle | Mohd Noor Akmal, Intan-Shameha Abdul Razak, Rozaihan Mansor, Aini Ideris, Abdul Rahman Omar, Jalila Abu, Mokrish Ajat, High-dose edible bird’s nest extract (EBN) upregulates LDL-R via suppression of HMGCR gene expression in HepG2 cell lines |
| title | High-dose edible bird’s nest extract (EBN) upregulates LDL-R via suppression of HMGCR gene expression in HepG2 cell lines |
| title_full | High-dose edible bird’s nest extract (EBN) upregulates LDL-R via suppression of HMGCR gene expression in HepG2 cell lines |
| title_fullStr | High-dose edible bird’s nest extract (EBN) upregulates LDL-R via suppression of HMGCR gene expression in HepG2 cell lines |
| title_full_unstemmed | High-dose edible bird’s nest extract (EBN) upregulates LDL-R via suppression of HMGCR gene expression in HepG2 cell lines |
| title_short | High-dose edible bird’s nest extract (EBN) upregulates LDL-R via suppression of HMGCR gene expression in HepG2 cell lines |
| title_sort | high dose edible bird s nest extract ebn upregulates ldl r via suppression of hmgcr gene expression in hepg2 cell lines |
| url | http://journalarticle.ukm.my/15939/1/9.pdf |
| work_keys_str_mv | AT mohdnoorakmal highdoseediblebirdsnestextractebnupregulatesldlrviasuppressionofhmgcrgeneexpressioninhepg2celllines AT intanshamehaabdulrazak highdoseediblebirdsnestextractebnupregulatesldlrviasuppressionofhmgcrgeneexpressioninhepg2celllines AT rozaihanmansor highdoseediblebirdsnestextractebnupregulatesldlrviasuppressionofhmgcrgeneexpressioninhepg2celllines AT ainiideris highdoseediblebirdsnestextractebnupregulatesldlrviasuppressionofhmgcrgeneexpressioninhepg2celllines AT abdulrahmanomar highdoseediblebirdsnestextractebnupregulatesldlrviasuppressionofhmgcrgeneexpressioninhepg2celllines AT jalilaabu highdoseediblebirdsnestextractebnupregulatesldlrviasuppressionofhmgcrgeneexpressioninhepg2celllines AT mokrishajat highdoseediblebirdsnestextractebnupregulatesldlrviasuppressionofhmgcrgeneexpressioninhepg2celllines |