| Summary: | Extracellular vesicles (EVs) are small entities that are released by most cell types. EVs are important form of
intercellular communication and a rich source of biomarkers for a wide variety of diseases. Many methods for EVs
isolation have been utilized, however, most of them have significant drawbacks including lengthy processing time, high
cost, shortfalls in selectivity and surface marker dependency. In consideration of these issues, this paper discussed on
the dielectrophoresis (DEP) microelectrode method designed to rapidly isolate EVs from its medium. The advantage of
this DEP microelectrode is the capability of isolating EVs using a droplet of 1 µL placed onto the microelectrode within
30 s and 20 V peak-to-peak (Vp-p) of alternating current (AC). The method used in the characterization of sample are
dynamic light scattering (DLS) and transmission electron microscopy (TEM); both prove the heterogeneity of EVs’
population and the EVs appear to be spherical with size ranging from 40 to 200 nm. The experimental results from this
preliminary experiment show that the DEP microelectrode was able to manipulate EVs as evidenced by the negative
dielectrophoresis (NDEP) fluorescent images. Further investigation of grid analysis conducted shows the consistency of
the theory and the results presented. Corrected Total Cell Fluorescence (CTCF) values from the grid analysis concluded
that the EVs were manipulated to the center of region of interest, (ROI). Therefore, this DEP technique suggests a rapid
strategy for EVs isolation from its medium in small quantity while maintaining accuracy and cost-effectivity.
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