Rapid manipulation of extracellular vesicles using dielectrophoretic mechanism
Extracellular vesicles (EVs) are small entities that are released by most cell types. EVs are important form of intercellular communication and a rich source of biomarkers for a wide variety of diseases. Many methods for EVs isolation have been utilized, however, most of them have significant draw...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/16156/1/3.pdf |
| _version_ | 1796932414582292480 |
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| author | Nur Mas Ayu Jamaludin, Muhammad Khairulanwar Abdul Rahim, Azrul Azlan Hamzah, Nadiah Abu, Muhamad Ramdzan Buyong, |
| author_facet | Nur Mas Ayu Jamaludin, Muhammad Khairulanwar Abdul Rahim, Azrul Azlan Hamzah, Nadiah Abu, Muhamad Ramdzan Buyong, |
| author_sort | Nur Mas Ayu Jamaludin, |
| collection | UKM |
| description | Extracellular vesicles (EVs) are small entities that are released by most cell types. EVs are important form of
intercellular communication and a rich source of biomarkers for a wide variety of diseases. Many methods for EVs
isolation have been utilized, however, most of them have significant drawbacks including lengthy processing time, high
cost, shortfalls in selectivity and surface marker dependency. In consideration of these issues, this paper discussed on
the dielectrophoresis (DEP) microelectrode method designed to rapidly isolate EVs from its medium. The advantage of
this DEP microelectrode is the capability of isolating EVs using a droplet of 1 µL placed onto the microelectrode within
30 s and 20 V peak-to-peak (Vp-p) of alternating current (AC). The method used in the characterization of sample are
dynamic light scattering (DLS) and transmission electron microscopy (TEM); both prove the heterogeneity of EVs’
population and the EVs appear to be spherical with size ranging from 40 to 200 nm. The experimental results from this
preliminary experiment show that the DEP microelectrode was able to manipulate EVs as evidenced by the negative
dielectrophoresis (NDEP) fluorescent images. Further investigation of grid analysis conducted shows the consistency of
the theory and the results presented. Corrected Total Cell Fluorescence (CTCF) values from the grid analysis concluded
that the EVs were manipulated to the center of region of interest, (ROI). Therefore, this DEP technique suggests a rapid
strategy for EVs isolation from its medium in small quantity while maintaining accuracy and cost-effectivity. |
| first_indexed | 2024-03-06T04:32:05Z |
| format | Article |
| id | ukm.eprints-16156 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:32:05Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-161562021-02-13T11:42:09Z http://journalarticle.ukm.my/16156/ Rapid manipulation of extracellular vesicles using dielectrophoretic mechanism Nur Mas Ayu Jamaludin, Muhammad Khairulanwar Abdul Rahim, Azrul Azlan Hamzah, Nadiah Abu, Muhamad Ramdzan Buyong, Extracellular vesicles (EVs) are small entities that are released by most cell types. EVs are important form of intercellular communication and a rich source of biomarkers for a wide variety of diseases. Many methods for EVs isolation have been utilized, however, most of them have significant drawbacks including lengthy processing time, high cost, shortfalls in selectivity and surface marker dependency. In consideration of these issues, this paper discussed on the dielectrophoresis (DEP) microelectrode method designed to rapidly isolate EVs from its medium. The advantage of this DEP microelectrode is the capability of isolating EVs using a droplet of 1 µL placed onto the microelectrode within 30 s and 20 V peak-to-peak (Vp-p) of alternating current (AC). The method used in the characterization of sample are dynamic light scattering (DLS) and transmission electron microscopy (TEM); both prove the heterogeneity of EVs’ population and the EVs appear to be spherical with size ranging from 40 to 200 nm. The experimental results from this preliminary experiment show that the DEP microelectrode was able to manipulate EVs as evidenced by the negative dielectrophoresis (NDEP) fluorescent images. Further investigation of grid analysis conducted shows the consistency of the theory and the results presented. Corrected Total Cell Fluorescence (CTCF) values from the grid analysis concluded that the EVs were manipulated to the center of region of interest, (ROI). Therefore, this DEP technique suggests a rapid strategy for EVs isolation from its medium in small quantity while maintaining accuracy and cost-effectivity. Penerbit Universiti Kebangsaan Malaysia 2020-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/16156/1/3.pdf Nur Mas Ayu Jamaludin, and Muhammad Khairulanwar Abdul Rahim, and Azrul Azlan Hamzah, and Nadiah Abu, and Muhamad Ramdzan Buyong, (2020) Rapid manipulation of extracellular vesicles using dielectrophoretic mechanism. Sains Malaysiana, 49 (12). pp. 2901-2912. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid49bil12_2020/KandunganJilid49Bil12_2020.html |
| spellingShingle | Nur Mas Ayu Jamaludin, Muhammad Khairulanwar Abdul Rahim, Azrul Azlan Hamzah, Nadiah Abu, Muhamad Ramdzan Buyong, Rapid manipulation of extracellular vesicles using dielectrophoretic mechanism |
| title | Rapid manipulation of extracellular vesicles using dielectrophoretic mechanism |
| title_full | Rapid manipulation of extracellular vesicles using dielectrophoretic mechanism |
| title_fullStr | Rapid manipulation of extracellular vesicles using dielectrophoretic mechanism |
| title_full_unstemmed | Rapid manipulation of extracellular vesicles using dielectrophoretic mechanism |
| title_short | Rapid manipulation of extracellular vesicles using dielectrophoretic mechanism |
| title_sort | rapid manipulation of extracellular vesicles using dielectrophoretic mechanism |
| url | http://journalarticle.ukm.my/16156/1/3.pdf |
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