Study of thermostable chitinase isolated and purified from Oryctes rhinoceros larvae gut
A chitinase-producing bacteria was locally isolated from rhinoceros beetle (Oryctes rhinoceros) larvae gut discovered in mushroom compost. A strain was selected from the source and labeled as RBLG1. It was identified using 16s rDNA sequencing and has strong homology to Bacillus cereus. Chitinase pro...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/16493/1/6.pdf |
| _version_ | 1796932475542306816 |
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| author | Aisyah Nabila Idris, Tang, Swee Seong Ahmad Faris Mohd Adnan, |
| author_facet | Aisyah Nabila Idris, Tang, Swee Seong Ahmad Faris Mohd Adnan, |
| author_sort | Aisyah Nabila Idris, |
| collection | UKM |
| description | A chitinase-producing bacteria was locally isolated from rhinoceros beetle (Oryctes rhinoceros) larvae gut discovered in mushroom compost. A strain was selected from the source and labeled as RBLG1. It was identified using 16s rDNA sequencing and has strong homology to Bacillus cereus. Chitinase produced from RBLG1 was purified using ammonium sulfate precipitation, ion exchange chromatography, and gel filtration. The purified chitinase from RBLG1 shows the homogeneity 1.9 fold after the gel filtration process. The purified chitinase was identified and analyzed using SDS-PAGE and the molecular mass from the strain was approximately 40 kDa. Further assays were performed to study the effect of temperature, pH, and metal ion on the purified enzyme. The purified enzyme shows highest chitinase activity at pH 4 and 60 °C. For metal ion test, chitinase from RBLG1 was unaffected when treated by various ions. Kinetic performance of chitinase using Michaelis-Menten equation shows Km and Vmax values were 2.3 mM and 0.0294 [P] mM/min, respectively. |
| first_indexed | 2024-03-06T04:33:01Z |
| format | Article |
| id | ukm.eprints-16493 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:33:01Z |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-164932021-04-28T04:25:11Z http://journalarticle.ukm.my/16493/ Study of thermostable chitinase isolated and purified from Oryctes rhinoceros larvae gut Aisyah Nabila Idris, Tang, Swee Seong Ahmad Faris Mohd Adnan, A chitinase-producing bacteria was locally isolated from rhinoceros beetle (Oryctes rhinoceros) larvae gut discovered in mushroom compost. A strain was selected from the source and labeled as RBLG1. It was identified using 16s rDNA sequencing and has strong homology to Bacillus cereus. Chitinase produced from RBLG1 was purified using ammonium sulfate precipitation, ion exchange chromatography, and gel filtration. The purified chitinase from RBLG1 shows the homogeneity 1.9 fold after the gel filtration process. The purified chitinase was identified and analyzed using SDS-PAGE and the molecular mass from the strain was approximately 40 kDa. Further assays were performed to study the effect of temperature, pH, and metal ion on the purified enzyme. The purified enzyme shows highest chitinase activity at pH 4 and 60 °C. For metal ion test, chitinase from RBLG1 was unaffected when treated by various ions. Kinetic performance of chitinase using Michaelis-Menten equation shows Km and Vmax values were 2.3 mM and 0.0294 [P] mM/min, respectively. Penerbit Universiti Kebangsaan Malaysia 2021-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/16493/1/6.pdf Aisyah Nabila Idris, and Tang, Swee Seong and Ahmad Faris Mohd Adnan, (2021) Study of thermostable chitinase isolated and purified from Oryctes rhinoceros larvae gut. Sains Malaysiana, 50 (2). pp. 339-349. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil2_2021/KandunganJilid50Bil2_2021.html |
| spellingShingle | Aisyah Nabila Idris, Tang, Swee Seong Ahmad Faris Mohd Adnan, Study of thermostable chitinase isolated and purified from Oryctes rhinoceros larvae gut |
| title | Study of thermostable chitinase isolated and purified from Oryctes rhinoceros larvae gut |
| title_full | Study of thermostable chitinase isolated and purified from Oryctes rhinoceros larvae gut |
| title_fullStr | Study of thermostable chitinase isolated and purified from Oryctes rhinoceros larvae gut |
| title_full_unstemmed | Study of thermostable chitinase isolated and purified from Oryctes rhinoceros larvae gut |
| title_short | Study of thermostable chitinase isolated and purified from Oryctes rhinoceros larvae gut |
| title_sort | study of thermostable chitinase isolated and purified from oryctes rhinoceros larvae gut |
| url | http://journalarticle.ukm.my/16493/1/6.pdf |
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