| Summary: | The P(3HB-co-4HB) is commonly used for biomedical applications. This is due to the desired mechanical properties as well
as biocompatibility, non-genotoxicity, and non-cytotoxicity of this copolymer. However, the production of P(3HB-co-4HB)
with a specific 4HB molar fraction is still limited. In this study, carbon precursors at different combinations and concentration
ratios have been used in the production of P(3HB-co-4HB) bioplastics using locally isolated Cupriavidus sp. TMT11. The
carbon precursors 1,6-hexandiol, 1,4-butanediol, and γ-butyrolactone were used to screen for high cell biomass, PHA content,
and 4HB molar fraction through Gas Chromatography (GC) analysis. Generally, the combination of various carbon precursors
showed an increase in cell biomass. The carbon combination of 1,4-butanediol and γ-butyrolactone at the ratio of 4:2 and
carbon combination of 1,6-hexanediol and γ-butyrolactone at a ratio of 1:5 showed high amount of cell biomass above 0.35
g/L on day 3. Nevertheless, the 4HB molar fraction of both the combination was recorded as 9±0.27 mol% and 14±1.1
mol% respectively. The lowest amount of cell biomass and PHA yield were recorded with the carbon combination of
1,6-hexanediol and 1,4-butanediol with the ratio 5:1 at 0.55±0.13 g/L. However, the highest 4HB molar fraction of 89.37±3.6
mol% 4HB was recorded with this combination. The 4HB molar fraction above 80 mol% was reported with a carbon
combination of 1,6-hexanediol +1,4-butanediol at 5:1, 1:1, and 2:4 ratio. The varying combination of carbon precursors
biosynthesized a wide range of 4HB molar fractions ranging from 9.07 to 89.37 mol% 4HB.
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