Characterization of Bacillus cereus isolates from local dairy products in Egypt
Bacillus cereus is an important, opportunistic, foodborne pathogen found in various dairy products. In this study, the prevalence, physiological characteristics, antimicrobial resistance profile, and enterotoxigenic genes (ces and hblA) of B. cereus were investigated in isolates from Egyptian dair...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/17531/1/13.pdf |
| _version_ | 1796932670253432832 |
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| author | Mohamed Aman, Ibrahim Al-Hawary, Ibrahim Ibrahim Khattab, Heba Mustafa Eldesoukey, Ibrahim Elsayed |
| author_facet | Mohamed Aman, Ibrahim Al-Hawary, Ibrahim Ibrahim Khattab, Heba Mustafa Eldesoukey, Ibrahim Elsayed |
| author_sort | Mohamed Aman, Ibrahim |
| collection | UKM |
| description | Bacillus cereus is an important, opportunistic, foodborne pathogen found in various dairy products. In this study, the
prevalence, physiological characteristics, antimicrobial resistance profile, and enterotoxigenic genes (ces and hblA) of
B. cereus were investigated in isolates from Egyptian dairy products. A total of 150 samples, including soft white cheese,
milk powder, and ultra-high temperature (UHT) milk (50 of each), were collected from local dairy stores in EL-Gharbia
governorate, Egypt from April 2019 to October 2019. Of these, 29 samples were contaminated with B. cereus (an overall
prevalence of 19.3%). Based on cultural, morphological, and biochemical characteristics, 48 isolates were detected
including 27 (56.25%) from soft white cheese, 9 (18.75%) from milk powder, and 12 (25%) from UHT milk. Antibiotic
susceptibility assessment showed that all isolates exhibited high sensitivity to amikacin, doxycyclin, gentamycin,
ciprofloxacin, while significant resistance to kanamycin, clindamycin, nalidixic acid, cephalothin, and sulphamethoxazole
was also observed. All isolates were examined for the presence of emetic (ces) and diarrheal (hblA) genes using the
PCR method; ces was detected in 12 (25%) isolates, hblA in 14 (29.2%) isolates, while 22 (45.8%) isolates did not harbor
either gene. These findings indicate the need for the application of adequate preventive measures and personnel
hygiene in dairy processing lines to minimize B. cereus load in final products. |
| first_indexed | 2024-03-06T04:35:59Z |
| format | Article |
| id | ukm.eprints-17531 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:35:59Z |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-175312021-10-26T04:58:11Z http://journalarticle.ukm.my/17531/ Characterization of Bacillus cereus isolates from local dairy products in Egypt Mohamed Aman, Ibrahim Al-Hawary, Ibrahim Ibrahim Khattab, Heba Mustafa Eldesoukey, Ibrahim Elsayed Bacillus cereus is an important, opportunistic, foodborne pathogen found in various dairy products. In this study, the prevalence, physiological characteristics, antimicrobial resistance profile, and enterotoxigenic genes (ces and hblA) of B. cereus were investigated in isolates from Egyptian dairy products. A total of 150 samples, including soft white cheese, milk powder, and ultra-high temperature (UHT) milk (50 of each), were collected from local dairy stores in EL-Gharbia governorate, Egypt from April 2019 to October 2019. Of these, 29 samples were contaminated with B. cereus (an overall prevalence of 19.3%). Based on cultural, morphological, and biochemical characteristics, 48 isolates were detected including 27 (56.25%) from soft white cheese, 9 (18.75%) from milk powder, and 12 (25%) from UHT milk. Antibiotic susceptibility assessment showed that all isolates exhibited high sensitivity to amikacin, doxycyclin, gentamycin, ciprofloxacin, while significant resistance to kanamycin, clindamycin, nalidixic acid, cephalothin, and sulphamethoxazole was also observed. All isolates were examined for the presence of emetic (ces) and diarrheal (hblA) genes using the PCR method; ces was detected in 12 (25%) isolates, hblA in 14 (29.2%) isolates, while 22 (45.8%) isolates did not harbor either gene. These findings indicate the need for the application of adequate preventive measures and personnel hygiene in dairy processing lines to minimize B. cereus load in final products. Penerbit Universiti Kebangsaan Malaysia 2021-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17531/1/13.pdf Mohamed Aman, Ibrahim and Al-Hawary, Ibrahim Ibrahim and Khattab, Heba Mustafa and Eldesoukey, Ibrahim Elsayed (2021) Characterization of Bacillus cereus isolates from local dairy products in Egypt. Sains Malaysiana, 50 (6). pp. 1663-1672. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil6_2021/KandunganJilid50Bil6_2021.html |
| spellingShingle | Mohamed Aman, Ibrahim Al-Hawary, Ibrahim Ibrahim Khattab, Heba Mustafa Eldesoukey, Ibrahim Elsayed Characterization of Bacillus cereus isolates from local dairy products in Egypt |
| title | Characterization of Bacillus cereus isolates from local dairy products in Egypt |
| title_full | Characterization of Bacillus cereus isolates from local dairy products in Egypt |
| title_fullStr | Characterization of Bacillus cereus isolates from local dairy products in Egypt |
| title_full_unstemmed | Characterization of Bacillus cereus isolates from local dairy products in Egypt |
| title_short | Characterization of Bacillus cereus isolates from local dairy products in Egypt |
| title_sort | characterization of bacillus cereus isolates from local dairy products in egypt |
| url | http://journalarticle.ukm.my/17531/1/13.pdf |
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