Effects of light illumination and subculture frequency on biomass production in cell suspension cultures of Clinacanthus nutans
Clinacanthus nutans (Burm. f.) Lindau is a medicinal plant rich in polyphenols and possesses multiple pharmacological properties. It is widely used as a side treatment for cancer and to prevent oxidative stress-related diseases. The present study aims to investigate the effects of light illuminati...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Penerbit Universiti Kebangsaan Malaysia
2021
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Online Access: | http://journalarticle.ukm.my/17907/1/197-204%2B%2B%2BBee%2BLynn%2BChew.pdf |
Summary: | Clinacanthus nutans (Burm. f.) Lindau is a medicinal plant rich in polyphenols and possesses multiple pharmacological
properties. It is widely used as a side treatment for cancer and to prevent oxidative stress-related diseases. The present
study aims to investigate the effects of light illumination and subculture frequency on the growth and proliferation of cells
in the suspension culture of C. nutans. Friable callus induced from leaf explants C. nutans were cultured in liquid Murashige
& Skoog medium supplemented with 0.25 mg/L 2, 4-dichlorophenoxyacetic acid, and 0.25 mg/L 6-benzylaminopurine to
initiate the cell suspension culture. The suspension cultures were incubated under different conditions (16 hr photoperiod
and continuous darkness). Subcultures were conducted every two weeks for ten subculture cycles. Results revealed that
higher cell biomass was obtained under the exposure of 16 hr photoperiod with the highest biomass production at the fifth
subculture cycle (growth index of 11.8) exhibiting stable growth from the eighth to the tenth subculture cycle. The suspension
cells showed stable growth rates up to the 10th subculture cycle producing small aggregates and single cells. The present
study indicated that light illumination significantly impacts the growth of suspension cultures for C. nutans in the production
of valuable secondary metabolites. |
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