Biological and analytical investigations of alpha-glucosidase inhibitory and anti-oxidant activities on selected Malaysian medicinal plants
This study was performed to establish anti-diabetic and anti-oxidant properties, and to carry out phytochemical analysis of selected local plants which are traditionally used as medicinal plants. Local plants involved in this study were Lawsonia inermis, Punica granatum, Dryobalanops aromatica, Zizi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/18060/1/11.pdf |
| _version_ | 1796932771683237888 |
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| author | Nooraqilah Che Rozenan, Nor Hisam Zamakshshari, Leong, Kok Hoong Najihah Mohd Hashim, Khadher Ahmad, Munirah Abd Razzak, Zulkifli Mohd Yusof, Khalijah Awang, Abd Aziz Ismail, Rozana Othman, |
| author_facet | Nooraqilah Che Rozenan, Nor Hisam Zamakshshari, Leong, Kok Hoong Najihah Mohd Hashim, Khadher Ahmad, Munirah Abd Razzak, Zulkifli Mohd Yusof, Khalijah Awang, Abd Aziz Ismail, Rozana Othman, |
| author_sort | Nooraqilah Che Rozenan, |
| collection | UKM |
| description | This study was performed to establish anti-diabetic and anti-oxidant properties, and to carry out phytochemical analysis of selected local plants which are traditionally used as medicinal plants. Local plants involved in this study were Lawsonia inermis, Punica granatum, Dryobalanops aromatica, Ziziphus mauritiana, and Ocimum basilicum. Solvent extraction was performed using maceration method with solvents of increasing polarity. Alpha-glucosidase inhibition assay was performed on all extracts to ascertain their anti-diabetic potentials. The extracts were screened for antioxidant activity using anti-oxidant assays (FRAP, DPPH, TAOC, ABTS, and BCB) and quantitative phytochemical analyses (TPC and TFC). Chemical profiling using LCMS and GCMS was performed on extracts with high biological activities. Methanol extracts of D. aromatica bark and leaves showed the most potent inhibition of alpha-glucosidase with IC50 values of 0.63 ± 0.03 μg/mL and 0.98 ± 0.02 μg/mL, respectively. Both extracts exhibited similar anti-oxidant activity on all five assays and possessed high phenolic contents with values of 266.79 and 261.69 GAE, respectively. The results obtained suggested that amongst the selected plants studied, D. aromatica showed high anti-oxidant activity and anti-diabetic activity (via inhibition of alpha-glucosidase). This is the first report that highlights the anti-diabetic potential of D. aromatica. |
| first_indexed | 2024-03-06T04:37:29Z |
| format | Article |
| id | ukm.eprints-18060 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:37:29Z |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-180602022-02-18T00:45:15Z http://journalarticle.ukm.my/18060/ Biological and analytical investigations of alpha-glucosidase inhibitory and anti-oxidant activities on selected Malaysian medicinal plants Nooraqilah Che Rozenan, Nor Hisam Zamakshshari, Leong, Kok Hoong Najihah Mohd Hashim, Khadher Ahmad, Munirah Abd Razzak, Zulkifli Mohd Yusof, Khalijah Awang, Abd Aziz Ismail, Rozana Othman, This study was performed to establish anti-diabetic and anti-oxidant properties, and to carry out phytochemical analysis of selected local plants which are traditionally used as medicinal plants. Local plants involved in this study were Lawsonia inermis, Punica granatum, Dryobalanops aromatica, Ziziphus mauritiana, and Ocimum basilicum. Solvent extraction was performed using maceration method with solvents of increasing polarity. Alpha-glucosidase inhibition assay was performed on all extracts to ascertain their anti-diabetic potentials. The extracts were screened for antioxidant activity using anti-oxidant assays (FRAP, DPPH, TAOC, ABTS, and BCB) and quantitative phytochemical analyses (TPC and TFC). Chemical profiling using LCMS and GCMS was performed on extracts with high biological activities. Methanol extracts of D. aromatica bark and leaves showed the most potent inhibition of alpha-glucosidase with IC50 values of 0.63 ± 0.03 μg/mL and 0.98 ± 0.02 μg/mL, respectively. Both extracts exhibited similar anti-oxidant activity on all five assays and possessed high phenolic contents with values of 266.79 and 261.69 GAE, respectively. The results obtained suggested that amongst the selected plants studied, D. aromatica showed high anti-oxidant activity and anti-diabetic activity (via inhibition of alpha-glucosidase). This is the first report that highlights the anti-diabetic potential of D. aromatica. Penerbit Universiti Kebangsaan Malaysia 2021-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18060/1/11.pdf Nooraqilah Che Rozenan, and Nor Hisam Zamakshshari, and Leong, Kok Hoong and Najihah Mohd Hashim, and Khadher Ahmad, and Munirah Abd Razzak, and Zulkifli Mohd Yusof, and Khalijah Awang, and Abd Aziz Ismail, and Rozana Othman, (2021) Biological and analytical investigations of alpha-glucosidase inhibitory and anti-oxidant activities on selected Malaysian medicinal plants. Sains Malaysiana, 50 (9). pp. 2625-2640. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil9_2021/KandunganJilid50Bil9_2021.html |
| spellingShingle | Nooraqilah Che Rozenan, Nor Hisam Zamakshshari, Leong, Kok Hoong Najihah Mohd Hashim, Khadher Ahmad, Munirah Abd Razzak, Zulkifli Mohd Yusof, Khalijah Awang, Abd Aziz Ismail, Rozana Othman, Biological and analytical investigations of alpha-glucosidase inhibitory and anti-oxidant activities on selected Malaysian medicinal plants |
| title | Biological and analytical investigations of alpha-glucosidase inhibitory and anti-oxidant activities on selected Malaysian medicinal plants |
| title_full | Biological and analytical investigations of alpha-glucosidase inhibitory and anti-oxidant activities on selected Malaysian medicinal plants |
| title_fullStr | Biological and analytical investigations of alpha-glucosidase inhibitory and anti-oxidant activities on selected Malaysian medicinal plants |
| title_full_unstemmed | Biological and analytical investigations of alpha-glucosidase inhibitory and anti-oxidant activities on selected Malaysian medicinal plants |
| title_short | Biological and analytical investigations of alpha-glucosidase inhibitory and anti-oxidant activities on selected Malaysian medicinal plants |
| title_sort | biological and analytical investigations of alpha glucosidase inhibitory and anti oxidant activities on selected malaysian medicinal plants |
| url | http://journalarticle.ukm.my/18060/1/11.pdf |
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