Phylogenetic relationships of the orchid genus Coelogyne in Peninsular Malaysia inferred from morphological characteristics and Internal Transcribed Spacer (ITS) sequence data
The phylogenetic relationships among the Peninsular Malaysian orchid genus Coelogyne were studied by morphological characteristics and sequence data of the internal transcribed region (ITS) from the nuclear ribosomal DNA (nrDNA). Coelogyne is a large genus of about 200 species distributed in pantrop...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/19153/1/2.pdf |
| _version_ | 1796933011614203904 |
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| author | Yoh, Kok-Hon Yong, Christina Seok-Yien Janna Ong Abdullah, Go, Rusea |
| author_facet | Yoh, Kok-Hon Yong, Christina Seok-Yien Janna Ong Abdullah, Go, Rusea |
| author_sort | Yoh, Kok-Hon |
| collection | UKM |
| description | The phylogenetic relationships among the Peninsular Malaysian orchid genus Coelogyne were studied by morphological characteristics and sequence data of the internal transcribed region (ITS) from the nuclear ribosomal DNA (nrDNA). Coelogyne is a large genus of about 200 species distributed in pantropical areas from the Himalayas, Sri Lanka, India, Southern China and throughout South East Asia to Papua New Guinea. The widely accepted previous classification system was exclusively based on floral morphology. There were very few molecular systematic studies of Coelogyne done in Peninsular Malaysia thus far. In this study, 59 Coelogyne samples were collected throughout Peninsular Malaysia and 57 of them were identified to the species level. To study the phylogeny of this genus, morphological characters were utilized together with molecular evidences to generate the systematic hypotheses. Cluster analysis was performed using both the vegetative and floral characters. The results showed that three sections of Peninsular Malaysian Coelogyne, namely Longifoliae, Speciosae, and Fuliginosae were sister groups which were more closely related by forming one clade by itself. Another clade consisted of four other sections, namely Flaccidae, Coelogynae, Tomentosae, and Verrucosae. Molecular phylogenies obtained by using the Neighbour-Joining method showed the close relationship between the sections Tomentosae and Verrucosae, whereas usage of the Maximum Likelihood method demonstrated that three sections, namely Longifoliae, Speciosae, and Fuliginosae, were sister groups since they formed a single clade. |
| first_indexed | 2024-03-06T04:40:38Z |
| format | Article |
| id | ukm.eprints-19153 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:40:38Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-191532022-08-01T03:43:19Z http://journalarticle.ukm.my/19153/ Phylogenetic relationships of the orchid genus Coelogyne in Peninsular Malaysia inferred from morphological characteristics and Internal Transcribed Spacer (ITS) sequence data Yoh, Kok-Hon Yong, Christina Seok-Yien Janna Ong Abdullah, Go, Rusea The phylogenetic relationships among the Peninsular Malaysian orchid genus Coelogyne were studied by morphological characteristics and sequence data of the internal transcribed region (ITS) from the nuclear ribosomal DNA (nrDNA). Coelogyne is a large genus of about 200 species distributed in pantropical areas from the Himalayas, Sri Lanka, India, Southern China and throughout South East Asia to Papua New Guinea. The widely accepted previous classification system was exclusively based on floral morphology. There were very few molecular systematic studies of Coelogyne done in Peninsular Malaysia thus far. In this study, 59 Coelogyne samples were collected throughout Peninsular Malaysia and 57 of them were identified to the species level. To study the phylogeny of this genus, morphological characters were utilized together with molecular evidences to generate the systematic hypotheses. Cluster analysis was performed using both the vegetative and floral characters. The results showed that three sections of Peninsular Malaysian Coelogyne, namely Longifoliae, Speciosae, and Fuliginosae were sister groups which were more closely related by forming one clade by itself. Another clade consisted of four other sections, namely Flaccidae, Coelogynae, Tomentosae, and Verrucosae. Molecular phylogenies obtained by using the Neighbour-Joining method showed the close relationship between the sections Tomentosae and Verrucosae, whereas usage of the Maximum Likelihood method demonstrated that three sections, namely Longifoliae, Speciosae, and Fuliginosae, were sister groups since they formed a single clade. Penerbit Universiti Kebangsaan Malaysia 2022-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/19153/1/2.pdf Yoh, Kok-Hon and Yong, Christina Seok-Yien and Janna Ong Abdullah, and Go, Rusea (2022) Phylogenetic relationships of the orchid genus Coelogyne in Peninsular Malaysia inferred from morphological characteristics and Internal Transcribed Spacer (ITS) sequence data. Sains Malaysiana, 51 (3). pp. 643-656. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil3_2022/KandunganJilid51Bil3_2022.html |
| spellingShingle | Yoh, Kok-Hon Yong, Christina Seok-Yien Janna Ong Abdullah, Go, Rusea Phylogenetic relationships of the orchid genus Coelogyne in Peninsular Malaysia inferred from morphological characteristics and Internal Transcribed Spacer (ITS) sequence data |
| title | Phylogenetic relationships of the orchid genus Coelogyne in Peninsular Malaysia inferred from morphological characteristics and Internal Transcribed Spacer (ITS) sequence data |
| title_full | Phylogenetic relationships of the orchid genus Coelogyne in Peninsular Malaysia inferred from morphological characteristics and Internal Transcribed Spacer (ITS) sequence data |
| title_fullStr | Phylogenetic relationships of the orchid genus Coelogyne in Peninsular Malaysia inferred from morphological characteristics and Internal Transcribed Spacer (ITS) sequence data |
| title_full_unstemmed | Phylogenetic relationships of the orchid genus Coelogyne in Peninsular Malaysia inferred from morphological characteristics and Internal Transcribed Spacer (ITS) sequence data |
| title_short | Phylogenetic relationships of the orchid genus Coelogyne in Peninsular Malaysia inferred from morphological characteristics and Internal Transcribed Spacer (ITS) sequence data |
| title_sort | phylogenetic relationships of the orchid genus coelogyne in peninsular malaysia inferred from morphological characteristics and internal transcribed spacer its sequence data |
| url | http://journalarticle.ukm.my/19153/1/2.pdf |
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