| Summary: | The fish embryo acute toxicity test (FET) was proposed as a promising alternative to the general practice of fish acute toxicity
test (FAT). To date, the available information on freshwater fish embryos in Southeast Asia is limited. Hence, this study aims
to present preliminary data on single and combined exposure effects of temperature and metals (zinc and cadmium) on the
embryonic development of Anabas testudineus. Over 80% of the embryos died after 10 h post-fertilization (hpf) in 1.59, 7.02,
and 13.0 mg/L cadmium treatments, whereas the same effect was only observed in 49.6 and 146 mg/L of zinc treatments as
early as 8 hpf. The optimum temperature for embryos ranged from 27 to 34 °C, with a survival rate greater than 90% was
recorded. The combined test of zinc + cadmium showed the additive effect with approximately 80% mortality at 12 hpf, while
the combination of temperature + cadmium had increased the mortality rate up to 100% at 16 hpf. The constant temperature even
at optimum rearing value was found to increase cadmium uptake in the embryos and magnified the concentration higher than in
water. Therefore, this study suggests climbing perch (Anabas testudineus) could be an alternative FET test model in this region.
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