A simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame
A potentiometric aspartame biosensor was fabricated by simply depositing the carboxylesterase (CES)-bonded poly(n-butyl acrylate-n-acryloxysuccimide) [CES-poly(nBA-NAS)] microspheres on a Ag/AgCl screen-printed pH selective electrode. The pH transducer was made from non-plasticized polyacrylate...
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/20646/1/14.pdf |
| _version_ | 1796933289925148672 |
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| author | Noor Izaanin Ramli, Lee, Yook Heng Tan, Ling Ling |
| author_facet | Noor Izaanin Ramli, Lee, Yook Heng Tan, Ling Ling |
| author_sort | Noor Izaanin Ramli, |
| collection | UKM |
| description | A potentiometric aspartame biosensor was fabricated by simply depositing the carboxylesterase (CES)-bonded
poly(n-butyl acrylate-n-acryloxysuccimide) [CES-poly(nBA-NAS)] microspheres on a Ag/AgCl screen-printed pH selective
electrode. The pH transducer was made from non-plasticized polyacrylate membrane containing a hydrogen ionophore
and lipophilic salt. The immobilized CES enzyme catalyzed the enzymatic hydrolysis of aspartame to L-aspartic
acid (L-Asp), L-phenylalanine and methanol. Potentiometric determination of aspartame concentration was performed
by quantifying the hydrogen ion concentration produced from L-Asp. The potentiometric determination of aspartame
exhibited good selectivity with near Nernstian response. The sensitivity of the biosensor was closed to the Nernstian
value, i.e., 50-52 mV decade-1 with a dynamic linear response range from 10-5 to 10-2 M and detection limit approaching
10-6 M. The aspartame biosensor demonstrated good repeatability and reproducibility with relative standard deviation
(RSD) of 1.9% and 1.6%, respectively (n=3). The potentiometric aspartame biosensor was demonstrated to be reliable for
determining aspartame content in sweetener samples and was comparable to the conventional high-performance liquid
chromatography (HPLC) method for aspartame analysis. |
| first_indexed | 2024-03-06T04:44:57Z |
| format | Article |
| id | ukm.eprints-20646 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:44:57Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-206462022-12-06T09:21:50Z http://journalarticle.ukm.my/20646/ A simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame Noor Izaanin Ramli, Lee, Yook Heng Tan, Ling Ling A potentiometric aspartame biosensor was fabricated by simply depositing the carboxylesterase (CES)-bonded poly(n-butyl acrylate-n-acryloxysuccimide) [CES-poly(nBA-NAS)] microspheres on a Ag/AgCl screen-printed pH selective electrode. The pH transducer was made from non-plasticized polyacrylate membrane containing a hydrogen ionophore and lipophilic salt. The immobilized CES enzyme catalyzed the enzymatic hydrolysis of aspartame to L-aspartic acid (L-Asp), L-phenylalanine and methanol. Potentiometric determination of aspartame concentration was performed by quantifying the hydrogen ion concentration produced from L-Asp. The potentiometric determination of aspartame exhibited good selectivity with near Nernstian response. The sensitivity of the biosensor was closed to the Nernstian value, i.e., 50-52 mV decade-1 with a dynamic linear response range from 10-5 to 10-2 M and detection limit approaching 10-6 M. The aspartame biosensor demonstrated good repeatability and reproducibility with relative standard deviation (RSD) of 1.9% and 1.6%, respectively (n=3). The potentiometric aspartame biosensor was demonstrated to be reliable for determining aspartame content in sweetener samples and was comparable to the conventional high-performance liquid chromatography (HPLC) method for aspartame analysis. Penerbit Universiti Kebangsaan Malaysia 2022-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/20646/1/14.pdf Noor Izaanin Ramli, and Lee, Yook Heng and Tan, Ling Ling (2022) A simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame. Sains Malaysiana, 51 (9). pp. 2913-2924. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil9_2022/KandunganJilid51Bil9_2022.html |
| spellingShingle | Noor Izaanin Ramli, Lee, Yook Heng Tan, Ling Ling A simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame |
| title | A simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame |
| title_full | A simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame |
| title_fullStr | A simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame |
| title_full_unstemmed | A simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame |
| title_short | A simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame |
| title_sort | simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame |
| url | http://journalarticle.ukm.my/20646/1/14.pdf |
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