The antimicrobial and antibiofilm potential of sweet basil essential oil on Streptococcus mutans and Staphylococcus aureus
The antimicrobial and antibiofilm activities of sweet basil essential oil (SBEO) against oral microorganisms, Streptococcus mutans (S. mutans) and Staphylococcus aureus (S. aureus) were determined in this study. The antimicrobial activities were evaluated using the disk diffusion method (DDM), where...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/21764/1/MAS%2024.pdf |
| _version_ | 1796933488139567104 |
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| author | Nurhayati Mohamad Zain, Nabilah Mohd Amin, Fatin Athirahusna Nordin, Junaidah Mahmud, Indah Mohd Amin, |
| author_facet | Nurhayati Mohamad Zain, Nabilah Mohd Amin, Fatin Athirahusna Nordin, Junaidah Mahmud, Indah Mohd Amin, |
| author_sort | Nurhayati Mohamad Zain, |
| collection | UKM |
| description | The antimicrobial and antibiofilm activities of sweet basil essential oil (SBEO) against oral microorganisms, Streptococcus mutans (S. mutans) and Staphylococcus aureus (S. aureus) were determined in this study. The antimicrobial activities were evaluated using the disk diffusion method (DDM), where Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were tested against both bacteria. Different formulations of SBEO (microemulsion, emulsion, water) were tested for biofilm dispersion assay on 24 h of preformed biofilm. Commercial chlorhexidine (CHX) 0.12% w/v was used as a positive control. The scanning electron microscope (SEM) was used to observe the changes on the treated surface. The data were analyzed using SPSS Version 27.0. A Kruskal-Wallis test followed by a Post-hoc Mann-Whitney U test was applied, and the level of significance was set at p<0.05. All formulations of SBEO showed antimicrobial activities against the tested microorganisms. Exposure to 2.5% (v/v) microemulsion for two min exhibited 42.56% and 32.10% (p<0.001) of biofilm dispersion for S. mutans and S. aureus, respectively. The SEM micrographs revealed the number of microorganisms on the treated group surface reduced compared to the negative controls. SBEO exerts an antimicrobial and antibiofilm effect on S. mutans and S. aureus. This finding suggests that the SBEO microemulsion has the potential to control planktonic S. mutans and S. aureus and their biofilm formation in the oral environment. |
| first_indexed | 2024-03-06T04:47:59Z |
| format | Article |
| id | ukm.eprints-21764 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:47:59Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-217642023-06-21T06:31:10Z http://journalarticle.ukm.my/21764/ The antimicrobial and antibiofilm potential of sweet basil essential oil on Streptococcus mutans and Staphylococcus aureus Nurhayati Mohamad Zain, Nabilah Mohd Amin, Fatin Athirahusna Nordin, Junaidah Mahmud, Indah Mohd Amin, The antimicrobial and antibiofilm activities of sweet basil essential oil (SBEO) against oral microorganisms, Streptococcus mutans (S. mutans) and Staphylococcus aureus (S. aureus) were determined in this study. The antimicrobial activities were evaluated using the disk diffusion method (DDM), where Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were tested against both bacteria. Different formulations of SBEO (microemulsion, emulsion, water) were tested for biofilm dispersion assay on 24 h of preformed biofilm. Commercial chlorhexidine (CHX) 0.12% w/v was used as a positive control. The scanning electron microscope (SEM) was used to observe the changes on the treated surface. The data were analyzed using SPSS Version 27.0. A Kruskal-Wallis test followed by a Post-hoc Mann-Whitney U test was applied, and the level of significance was set at p<0.05. All formulations of SBEO showed antimicrobial activities against the tested microorganisms. Exposure to 2.5% (v/v) microemulsion for two min exhibited 42.56% and 32.10% (p<0.001) of biofilm dispersion for S. mutans and S. aureus, respectively. The SEM micrographs revealed the number of microorganisms on the treated group surface reduced compared to the negative controls. SBEO exerts an antimicrobial and antibiofilm effect on S. mutans and S. aureus. This finding suggests that the SBEO microemulsion has the potential to control planktonic S. mutans and S. aureus and their biofilm formation in the oral environment. Penerbit Universiti Kebangsaan Malaysia 2022 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/21764/1/MAS%2024.pdf Nurhayati Mohamad Zain, and Nabilah Mohd Amin, and Fatin Athirahusna Nordin, and Junaidah Mahmud, and Indah Mohd Amin, (2022) The antimicrobial and antibiofilm potential of sweet basil essential oil on Streptococcus mutans and Staphylococcus aureus. Malaysian Applied Biology, 51 (4). pp. 213-220. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/index |
| spellingShingle | Nurhayati Mohamad Zain, Nabilah Mohd Amin, Fatin Athirahusna Nordin, Junaidah Mahmud, Indah Mohd Amin, The antimicrobial and antibiofilm potential of sweet basil essential oil on Streptococcus mutans and Staphylococcus aureus |
| title | The antimicrobial and antibiofilm potential of sweet basil essential oil on Streptococcus mutans and Staphylococcus aureus |
| title_full | The antimicrobial and antibiofilm potential of sweet basil essential oil on Streptococcus mutans and Staphylococcus aureus |
| title_fullStr | The antimicrobial and antibiofilm potential of sweet basil essential oil on Streptococcus mutans and Staphylococcus aureus |
| title_full_unstemmed | The antimicrobial and antibiofilm potential of sweet basil essential oil on Streptococcus mutans and Staphylococcus aureus |
| title_short | The antimicrobial and antibiofilm potential of sweet basil essential oil on Streptococcus mutans and Staphylococcus aureus |
| title_sort | antimicrobial and antibiofilm potential of sweet basil essential oil on streptococcus mutans and staphylococcus aureus |
| url | http://journalarticle.ukm.my/21764/1/MAS%2024.pdf |
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