Persicaria minor F-box gene PmF-box1 indirectly affects Arabidopsis thaliana LOX-HPL pathway for green leaf volatile production
Green leaf volatiles (GLVs) play an essential role in plant defence, plant-plant interaction and plant-insect interaction. The plant releases GLVs and inhibits the growth and propagation of plant pathogens. In this study, overexpression of PmF-box1 in wild type A. thaliana showed the downregulation...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2023
|
| Online Access: | http://journalarticle.ukm.my/22784/1/SEN%204.pdf |
| _version_ | 1796933668395024384 |
|---|---|
| author | Nur-Athirah Abd-Hamid, Muhammad Naeem-Ul-Hassan, Zamri Zainal, Ismanizan Ismail, |
| author_facet | Nur-Athirah Abd-Hamid, Muhammad Naeem-Ul-Hassan, Zamri Zainal, Ismanizan Ismail, |
| author_sort | Nur-Athirah Abd-Hamid, |
| collection | UKM |
| description | Green leaf volatiles (GLVs) play an essential role in plant defence, plant-plant interaction and plant-insect interaction. The plant releases GLVs and inhibits the growth and propagation of plant pathogens. In this study, overexpression of PmF-box1 in wild type A. thaliana showed the downregulation of genes involved in the lipoxygenase-hydroperoxide lyase (LOX-HPL) pathway, which contributes to the biosynthesis of GLVs. It resulted in a marked reduction of hexanal production in the PmF-box1-overexpressing plant. The expression pattern of LOX-HPL branch genes in the kelch-repeat modified PmF-box1 (KMF)-overexpressing plant showed a pattern much closer to the expression of LOX-HPL branch genes in the vector control (VC) plant. It was shown that the functional KMF protein sequence was not responsible for the significant reduction of all GLVs including hexanal, 1-hexanol, (Z)-3-hexen-1-ol, and the carbon 5 (C5) volatile, 1-penten-3-ol, in plants overexpressing KMF. Furthermore, this study also showed that the relative proportion of production of 1-penten-3-ol to hexanal was higher in the PmF-box1-overexpressing plant. Based on the current comparative literature search, PmF-box1 does not appear to interact directly with the proteins or transcription factors of the LOX-HPL pathway. On the other hand, PmF-box1 interacts with SAMS1, which subsequently influences the HPL pathway enzyme genes. Thus, this study highlights the potential roles of PmF-box1 in the manipulation of GLV productions. |
| first_indexed | 2024-03-06T04:50:45Z |
| format | Article |
| id | ukm.eprints-22784 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:50:45Z |
| publishDate | 2023 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-227842024-01-03T02:29:05Z http://journalarticle.ukm.my/22784/ Persicaria minor F-box gene PmF-box1 indirectly affects Arabidopsis thaliana LOX-HPL pathway for green leaf volatile production Nur-Athirah Abd-Hamid, Muhammad Naeem-Ul-Hassan, Zamri Zainal, Ismanizan Ismail, Green leaf volatiles (GLVs) play an essential role in plant defence, plant-plant interaction and plant-insect interaction. The plant releases GLVs and inhibits the growth and propagation of plant pathogens. In this study, overexpression of PmF-box1 in wild type A. thaliana showed the downregulation of genes involved in the lipoxygenase-hydroperoxide lyase (LOX-HPL) pathway, which contributes to the biosynthesis of GLVs. It resulted in a marked reduction of hexanal production in the PmF-box1-overexpressing plant. The expression pattern of LOX-HPL branch genes in the kelch-repeat modified PmF-box1 (KMF)-overexpressing plant showed a pattern much closer to the expression of LOX-HPL branch genes in the vector control (VC) plant. It was shown that the functional KMF protein sequence was not responsible for the significant reduction of all GLVs including hexanal, 1-hexanol, (Z)-3-hexen-1-ol, and the carbon 5 (C5) volatile, 1-penten-3-ol, in plants overexpressing KMF. Furthermore, this study also showed that the relative proportion of production of 1-penten-3-ol to hexanal was higher in the PmF-box1-overexpressing plant. Based on the current comparative literature search, PmF-box1 does not appear to interact directly with the proteins or transcription factors of the LOX-HPL pathway. On the other hand, PmF-box1 interacts with SAMS1, which subsequently influences the HPL pathway enzyme genes. Thus, this study highlights the potential roles of PmF-box1 in the manipulation of GLV productions. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/22784/1/SEN%204.pdf Nur-Athirah Abd-Hamid, and Muhammad Naeem-Ul-Hassan, and Zamri Zainal, and Ismanizan Ismail, (2023) Persicaria minor F-box gene PmF-box1 indirectly affects Arabidopsis thaliana LOX-HPL pathway for green leaf volatile production. Sains Malaysiana, 52 (6). pp. 1649-1670. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol52num6_2023/contentsVol52num6_2023.html |
| spellingShingle | Nur-Athirah Abd-Hamid, Muhammad Naeem-Ul-Hassan, Zamri Zainal, Ismanizan Ismail, Persicaria minor F-box gene PmF-box1 indirectly affects Arabidopsis thaliana LOX-HPL pathway for green leaf volatile production |
| title | Persicaria minor F-box gene PmF-box1 indirectly affects Arabidopsis thaliana LOX-HPL pathway for green leaf volatile production |
| title_full | Persicaria minor F-box gene PmF-box1 indirectly affects Arabidopsis thaliana LOX-HPL pathway for green leaf volatile production |
| title_fullStr | Persicaria minor F-box gene PmF-box1 indirectly affects Arabidopsis thaliana LOX-HPL pathway for green leaf volatile production |
| title_full_unstemmed | Persicaria minor F-box gene PmF-box1 indirectly affects Arabidopsis thaliana LOX-HPL pathway for green leaf volatile production |
| title_short | Persicaria minor F-box gene PmF-box1 indirectly affects Arabidopsis thaliana LOX-HPL pathway for green leaf volatile production |
| title_sort | persicaria minor f box gene pmf box1 indirectly affects arabidopsis thaliana lox hpl pathway for green leaf volatile production |
| url | http://journalarticle.ukm.my/22784/1/SEN%204.pdf |
| work_keys_str_mv | AT nurathirahabdhamid persicariaminorfboxgenepmfbox1indirectlyaffectsarabidopsisthalianaloxhplpathwayforgreenleafvolatileproduction AT muhammadnaeemulhassan persicariaminorfboxgenepmfbox1indirectlyaffectsarabidopsisthalianaloxhplpathwayforgreenleafvolatileproduction AT zamrizainal persicariaminorfboxgenepmfbox1indirectlyaffectsarabidopsisthalianaloxhplpathwayforgreenleafvolatileproduction AT ismanizanismail persicariaminorfboxgenepmfbox1indirectlyaffectsarabidopsisthalianaloxhplpathwayforgreenleafvolatileproduction |