Dioscorea alata extract as anti-cancer agent by regulating genetic mutations, apoptosis, and cell proliferation: in vitro and in silico studies
Dioscorea alata (DA) tubers contain some compounds of potential anti-cancer agents. However, despite its potential, there has been limited investigation on the potential anti-cancer agent of DA, especially in breast cancer cells. Therefore, this study aims to investigate the anti-cancer effect of et...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2023
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| Online Access: | http://journalarticle.ukm.my/23094/1/SMS%2015.pdf |
| _version_ | 1796933724945776640 |
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| author | Nurul Makiyah, Sri Nabawiyati Christina, Yuyun Ika Ahkam, Ahmad Hafidul |
| author_facet | Nurul Makiyah, Sri Nabawiyati Christina, Yuyun Ika Ahkam, Ahmad Hafidul |
| author_sort | Nurul Makiyah, Sri Nabawiyati |
| collection | UKM |
| description | Dioscorea alata (DA) tubers contain some compounds of potential anti-cancer agents. However, despite its potential, there has been limited investigation on the potential anti-cancer agent of DA, especially in breast cancer cells. Therefore, this study aims to investigate the anti-cancer effect of ethanolic extract of DA tubers against the MCF-7 cell lines in vitro and in silico. The in vitro study was carried out by cytotoxicity effect using MTT assay. The in silico study examined active ingredients, determined anti-cancer activity, analyzed the protein target, protein-protein interactions, and molecular docking. The study results showed that the high-concentration ethanolic extract of DA tubers exhibited the highest decrease in MCF-7 cell viability with an IC50 value of 50.98 g/mL and can be categorized as a promising anti-cancer agent. As many as 34 active compounds belonging to anthocyanins, saponins, and flavonoids were screened. Active compounds of DA tubers with a violation score < 2 were analyzed for their biological potentials. Ten compounds of DA with the highest potency were selected for docking analysis with the targeted protein EGFR. The in-silico study showed that 21 bioactive compounds contribute to the anti-cancer activity of DA. Chlorogenic acid is the most promising bioactive compound as an anti-cancer since it is the most similar to the anti-cancer drug. Meanwhile, dihydroquercetin has a low binding affinity value, requiring less energy to bind to proteins in cancer signaling indicated that this compound is promising as anti-cancer. This study suggested that the ethanolic extract of DA tubers can be considered an anti-cancer agent against the MCF-7 cell line in vitro by regulating genetic mutations, apoptosis, and cell proliferation. |
| first_indexed | 2024-03-06T04:51:36Z |
| format | Article |
| id | ukm.eprints-23094 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:51:36Z |
| publishDate | 2023 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-230942024-02-19T08:33:14Z http://journalarticle.ukm.my/23094/ Dioscorea alata extract as anti-cancer agent by regulating genetic mutations, apoptosis, and cell proliferation: in vitro and in silico studies Nurul Makiyah, Sri Nabawiyati Christina, Yuyun Ika Ahkam, Ahmad Hafidul Dioscorea alata (DA) tubers contain some compounds of potential anti-cancer agents. However, despite its potential, there has been limited investigation on the potential anti-cancer agent of DA, especially in breast cancer cells. Therefore, this study aims to investigate the anti-cancer effect of ethanolic extract of DA tubers against the MCF-7 cell lines in vitro and in silico. The in vitro study was carried out by cytotoxicity effect using MTT assay. The in silico study examined active ingredients, determined anti-cancer activity, analyzed the protein target, protein-protein interactions, and molecular docking. The study results showed that the high-concentration ethanolic extract of DA tubers exhibited the highest decrease in MCF-7 cell viability with an IC50 value of 50.98 g/mL and can be categorized as a promising anti-cancer agent. As many as 34 active compounds belonging to anthocyanins, saponins, and flavonoids were screened. Active compounds of DA tubers with a violation score < 2 were analyzed for their biological potentials. Ten compounds of DA with the highest potency were selected for docking analysis with the targeted protein EGFR. The in-silico study showed that 21 bioactive compounds contribute to the anti-cancer activity of DA. Chlorogenic acid is the most promising bioactive compound as an anti-cancer since it is the most similar to the anti-cancer drug. Meanwhile, dihydroquercetin has a low binding affinity value, requiring less energy to bind to proteins in cancer signaling indicated that this compound is promising as anti-cancer. This study suggested that the ethanolic extract of DA tubers can be considered an anti-cancer agent against the MCF-7 cell line in vitro by regulating genetic mutations, apoptosis, and cell proliferation. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/23094/1/SMS%2015.pdf Nurul Makiyah, Sri Nabawiyati and Christina, Yuyun Ika and Ahkam, Ahmad Hafidul (2023) Dioscorea alata extract as anti-cancer agent by regulating genetic mutations, apoptosis, and cell proliferation: in vitro and in silico studies. Sains Malaysiana, 52 (9). pp. 2657-2671. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol52num9_2023/contentsVol52num9_2023.html |
| spellingShingle | Nurul Makiyah, Sri Nabawiyati Christina, Yuyun Ika Ahkam, Ahmad Hafidul Dioscorea alata extract as anti-cancer agent by regulating genetic mutations, apoptosis, and cell proliferation: in vitro and in silico studies |
| title | Dioscorea alata extract as anti-cancer agent by regulating genetic mutations, apoptosis, and cell proliferation: in vitro and in silico studies |
| title_full | Dioscorea alata extract as anti-cancer agent by regulating genetic mutations, apoptosis, and cell proliferation: in vitro and in silico studies |
| title_fullStr | Dioscorea alata extract as anti-cancer agent by regulating genetic mutations, apoptosis, and cell proliferation: in vitro and in silico studies |
| title_full_unstemmed | Dioscorea alata extract as anti-cancer agent by regulating genetic mutations, apoptosis, and cell proliferation: in vitro and in silico studies |
| title_short | Dioscorea alata extract as anti-cancer agent by regulating genetic mutations, apoptosis, and cell proliferation: in vitro and in silico studies |
| title_sort | dioscorea alata extract as anti cancer agent by regulating genetic mutations apoptosis and cell proliferation in vitro and in silico studies |
| url | http://journalarticle.ukm.my/23094/1/SMS%2015.pdf |
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