Production of gamma aminobutyric acid (GABA)- Miso using Candida parapsilosis Isolated from a commercial soy sauce moromi

A newly identified yeast, Candida parapsilosis strain KBC (CPSKBC) was tested for its GABA-producing potential in miso fermentation and consumer acceptability towards the GABA-miso was evaluated through sensory analysis. Three different formulations were used over one month to make the GABA-miso: fo...

Full description

Bibliographic Details
Main Authors: Chong, Shin Yee, Zul Ilham, Nurul Aqilah Mohd Zaini, Muhamad Hafiz Abd Rahim, Siva Raseetha, Raposo, António, Alarifi, Sehad N., Mohd Nizam Lani, Wan Abd Al Qadr Imad Wan-Mohtar
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2023
Online Access:http://journalarticle.ukm.my/23356/1/SD%207.pdf
_version_ 1796933774730067968
author Chong, Shin Yee
Zul Ilham,
Nurul Aqilah Mohd Zaini,
Muhamad Hafiz Abd Rahim,
Siva Raseetha,
Raposo, António
Alarifi, Sehad N.
Mohd Nizam Lani,
Wan Abd Al Qadr Imad Wan-Mohtar,
author_facet Chong, Shin Yee
Zul Ilham,
Nurul Aqilah Mohd Zaini,
Muhamad Hafiz Abd Rahim,
Siva Raseetha,
Raposo, António
Alarifi, Sehad N.
Mohd Nizam Lani,
Wan Abd Al Qadr Imad Wan-Mohtar,
author_sort Chong, Shin Yee
collection UKM
description A newly identified yeast, Candida parapsilosis strain KBC (CPSKBC) was tested for its GABA-producing potential in miso fermentation and consumer acceptability towards the GABA-miso was evaluated through sensory analysis. Three different formulations were used over one month to make the GABA-miso: formulation 1 (F1) used soybeans, rice koji, and miso starter; formulation 2 (F2) used soybeans, rice koji, and CPSKBC suspension; and formula 3 (F3) used soybeans, rice koji, miso starter, and CPSKBC suspension. High Performance Liquid Chromatography (HPLC) was used to measure the gamma-aminobutyric acid (GABA) concentration of the miso produced. F3 produced the highest GABA (36.73 mg/L), followed by F2 (34.54 mg/L) and F1 (28.42 mg/L). Sensory evaluation was conducted with 60 panelists using a 9-point hedonic scale. For all the sensory attributes evaluated (appearance, color, odor, taste and overall acceptability), the results showed no significant differences (p >0.05) between groups of miso (F1, F2, and F3) and commercial miso (C1). We can conclude that our products (F1, F2, and F3) matched with commercially produced miso in the market. This study demonstrated that CPSKBC was able to produce GABA-miso (F2 and F3) which is acceptable to the consumer.
first_indexed 2024-04-21T08:53:09Z
format Article
id ukm.eprints-23356
institution Universiti Kebangsaan Malaysia
language English
last_indexed 2024-04-21T08:53:09Z
publishDate 2023
publisher Penerbit Universiti Kebangsaan Malaysia
record_format dspace
spelling ukm.eprints-233562024-04-17T06:45:49Z http://journalarticle.ukm.my/23356/ Production of gamma aminobutyric acid (GABA)- Miso using Candida parapsilosis Isolated from a commercial soy sauce moromi Chong, Shin Yee Zul Ilham, Nurul Aqilah Mohd Zaini, Muhamad Hafiz Abd Rahim, Siva Raseetha, Raposo, António Alarifi, Sehad N. Mohd Nizam Lani, Wan Abd Al Qadr Imad Wan-Mohtar, A newly identified yeast, Candida parapsilosis strain KBC (CPSKBC) was tested for its GABA-producing potential in miso fermentation and consumer acceptability towards the GABA-miso was evaluated through sensory analysis. Three different formulations were used over one month to make the GABA-miso: formulation 1 (F1) used soybeans, rice koji, and miso starter; formulation 2 (F2) used soybeans, rice koji, and CPSKBC suspension; and formula 3 (F3) used soybeans, rice koji, miso starter, and CPSKBC suspension. High Performance Liquid Chromatography (HPLC) was used to measure the gamma-aminobutyric acid (GABA) concentration of the miso produced. F3 produced the highest GABA (36.73 mg/L), followed by F2 (34.54 mg/L) and F1 (28.42 mg/L). Sensory evaluation was conducted with 60 panelists using a 9-point hedonic scale. For all the sensory attributes evaluated (appearance, color, odor, taste and overall acceptability), the results showed no significant differences (p >0.05) between groups of miso (F1, F2, and F3) and commercial miso (C1). We can conclude that our products (F1, F2, and F3) matched with commercially produced miso in the market. This study demonstrated that CPSKBC was able to produce GABA-miso (F2 and F3) which is acceptable to the consumer. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/23356/1/SD%207.pdf Chong, Shin Yee and Zul Ilham, and Nurul Aqilah Mohd Zaini, and Muhamad Hafiz Abd Rahim, and Siva Raseetha, and Raposo, António and Alarifi, Sehad N. and Mohd Nizam Lani, and Wan Abd Al Qadr Imad Wan-Mohtar, (2023) Production of gamma aminobutyric acid (GABA)- Miso using Candida parapsilosis Isolated from a commercial soy sauce moromi. Sains Malaysiana, 52 (12). pp. 3437-3447. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol52num12_2023/contentsVol52num12_2023.html
spellingShingle Chong, Shin Yee
Zul Ilham,
Nurul Aqilah Mohd Zaini,
Muhamad Hafiz Abd Rahim,
Siva Raseetha,
Raposo, António
Alarifi, Sehad N.
Mohd Nizam Lani,
Wan Abd Al Qadr Imad Wan-Mohtar,
Production of gamma aminobutyric acid (GABA)- Miso using Candida parapsilosis Isolated from a commercial soy sauce moromi
title Production of gamma aminobutyric acid (GABA)- Miso using Candida parapsilosis Isolated from a commercial soy sauce moromi
title_full Production of gamma aminobutyric acid (GABA)- Miso using Candida parapsilosis Isolated from a commercial soy sauce moromi
title_fullStr Production of gamma aminobutyric acid (GABA)- Miso using Candida parapsilosis Isolated from a commercial soy sauce moromi
title_full_unstemmed Production of gamma aminobutyric acid (GABA)- Miso using Candida parapsilosis Isolated from a commercial soy sauce moromi
title_short Production of gamma aminobutyric acid (GABA)- Miso using Candida parapsilosis Isolated from a commercial soy sauce moromi
title_sort production of gamma aminobutyric acid gaba miso using candida parapsilosis isolated from a commercial soy sauce moromi
url http://journalarticle.ukm.my/23356/1/SD%207.pdf
work_keys_str_mv AT chongshinyee productionofgammaaminobutyricacidgabamisousingcandidaparapsilosisisolatedfromacommercialsoysaucemoromi
AT zulilham productionofgammaaminobutyricacidgabamisousingcandidaparapsilosisisolatedfromacommercialsoysaucemoromi
AT nurulaqilahmohdzaini productionofgammaaminobutyricacidgabamisousingcandidaparapsilosisisolatedfromacommercialsoysaucemoromi
AT muhamadhafizabdrahim productionofgammaaminobutyricacidgabamisousingcandidaparapsilosisisolatedfromacommercialsoysaucemoromi
AT sivaraseetha productionofgammaaminobutyricacidgabamisousingcandidaparapsilosisisolatedfromacommercialsoysaucemoromi
AT raposoantonio productionofgammaaminobutyricacidgabamisousingcandidaparapsilosisisolatedfromacommercialsoysaucemoromi
AT alarifisehadn productionofgammaaminobutyricacidgabamisousingcandidaparapsilosisisolatedfromacommercialsoysaucemoromi
AT mohdnizamlani productionofgammaaminobutyricacidgabamisousingcandidaparapsilosisisolatedfromacommercialsoysaucemoromi
AT wanabdalqadrimadwanmohtar productionofgammaaminobutyricacidgabamisousingcandidaparapsilosisisolatedfromacommercialsoysaucemoromi