Investigation of phosphorylated and O-glycosylated proteins in gestational diabetes mellitus
Gestational Diabetes Mellitus (GDM) is a common but temporary type of diabetes that develops among 10-20% of all pregnant women. It is a major cause of several pre- and post-pregnancy diseases in both mother and offspring. The main causative is altered pregnancy hormones that lead to deficient gluco...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2024
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| Online Access: | http://journalarticle.ukm.my/23753/1/SMT%2015.pdf |
| _version_ | 1811136849731649536 |
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| author | Kaleem, Afshan Anwar, Tehreem Iftikhar, Aqsa Abdullah, Roheena Mehwish Iqtedar, Mehwish Aftab, Mahwish |
| author_facet | Kaleem, Afshan Anwar, Tehreem Iftikhar, Aqsa Abdullah, Roheena Mehwish Iqtedar, Mehwish Aftab, Mahwish |
| author_sort | Kaleem, Afshan |
| collection | UKM |
| description | Gestational Diabetes Mellitus (GDM) is a common but temporary type of diabetes that develops among 10-20% of all pregnant women. It is a major cause of several pre- and post-pregnancy diseases in both mother and offspring. The main causative is altered pregnancy hormones that lead to deficient glucose metabolism. Complications can be preeclampsia, caesarean sections and cardiovascular diseases. This study was designed to determine the phosphorylated and glycosylated proteins present in GDM and to investigate the role of their post-translational modifications (PTMs) in the pathogenesis of GDM. The study was performed on 30 blood samples from pregnant diabetic women (diseased), 30 pregnant non-diabetic women (control) and 30 women without pregnancy and gestational diabetes (normal). Protein extraction was done from blood plasma, and phosphorylated and glycosylated proteins were determined by EnzymeLinked Immuno-Sorbent Assay (ELISA) by use of specific antibodies. This study showed that proteins found in GDM are highly phosphorylated and O-glycosylated (p<0.01). Furthermore, a bioinformatic investigation was done, which showed that blood coagulation proteins such as the Fibrinogen alpha chain and lipid metabolism-regulating proteins apolipoprotein E, L1 and C-III showed a high potential for phosphorylation, which suggests that phosphorylation can be used as a therapeutic marker in GDM. |
| first_indexed | 2024-09-24T01:28:15Z |
| format | Article |
| id | ukm.eprints-23753 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-09-24T01:28:15Z |
| publishDate | 2024 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-237532024-07-04T04:10:10Z http://journalarticle.ukm.my/23753/ Investigation of phosphorylated and O-glycosylated proteins in gestational diabetes mellitus Kaleem, Afshan Anwar, Tehreem Iftikhar, Aqsa Abdullah, Roheena Mehwish Iqtedar, Mehwish Aftab, Mahwish Gestational Diabetes Mellitus (GDM) is a common but temporary type of diabetes that develops among 10-20% of all pregnant women. It is a major cause of several pre- and post-pregnancy diseases in both mother and offspring. The main causative is altered pregnancy hormones that lead to deficient glucose metabolism. Complications can be preeclampsia, caesarean sections and cardiovascular diseases. This study was designed to determine the phosphorylated and glycosylated proteins present in GDM and to investigate the role of their post-translational modifications (PTMs) in the pathogenesis of GDM. The study was performed on 30 blood samples from pregnant diabetic women (diseased), 30 pregnant non-diabetic women (control) and 30 women without pregnancy and gestational diabetes (normal). Protein extraction was done from blood plasma, and phosphorylated and glycosylated proteins were determined by EnzymeLinked Immuno-Sorbent Assay (ELISA) by use of specific antibodies. This study showed that proteins found in GDM are highly phosphorylated and O-glycosylated (p<0.01). Furthermore, a bioinformatic investigation was done, which showed that blood coagulation proteins such as the Fibrinogen alpha chain and lipid metabolism-regulating proteins apolipoprotein E, L1 and C-III showed a high potential for phosphorylation, which suggests that phosphorylation can be used as a therapeutic marker in GDM. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/23753/1/SMT%2015.pdf Kaleem, Afshan and Anwar, Tehreem and Iftikhar, Aqsa and Abdullah, Roheena and Mehwish Iqtedar, Mehwish and Aftab, Mahwish (2024) Investigation of phosphorylated and O-glycosylated proteins in gestational diabetes mellitus. Sains Malaysiana, 53 (3). pp. 681-689. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num3_2024/contentsVol53num3_2024.html |
| spellingShingle | Kaleem, Afshan Anwar, Tehreem Iftikhar, Aqsa Abdullah, Roheena Mehwish Iqtedar, Mehwish Aftab, Mahwish Investigation of phosphorylated and O-glycosylated proteins in gestational diabetes mellitus |
| title | Investigation of phosphorylated and O-glycosylated proteins in gestational diabetes mellitus |
| title_full | Investigation of phosphorylated and O-glycosylated proteins in gestational diabetes mellitus |
| title_fullStr | Investigation of phosphorylated and O-glycosylated proteins in gestational diabetes mellitus |
| title_full_unstemmed | Investigation of phosphorylated and O-glycosylated proteins in gestational diabetes mellitus |
| title_short | Investigation of phosphorylated and O-glycosylated proteins in gestational diabetes mellitus |
| title_sort | investigation of phosphorylated and o glycosylated proteins in gestational diabetes mellitus |
| url | http://journalarticle.ukm.my/23753/1/SMT%2015.pdf |
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