Mechanism of damnacanthal induced apoptosis in CEM-SS cell line
Leukaemia, is cancer of organs that is responsible to produce blood specifically the lymphatic system and bone marrow. Due to the harsh effects of currently used cancer drugs, damnacanthal, an anthraquinone obtained from the roots of Morinda elliptica is tested as a potential anticancer agent. This...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2024
|
| Online Access: | http://journalarticle.ukm.my/24502/1/SS%2020.pdf |
| _version_ | 1817926846333845504 |
|---|---|
| author | Banulata Gopalsamy, Saiful Yazan Latifah, Hisyam Abdul Hamid, |
| author_facet | Banulata Gopalsamy, Saiful Yazan Latifah, Hisyam Abdul Hamid, |
| author_sort | Banulata Gopalsamy, |
| collection | UKM |
| description | Leukaemia, is cancer of organs that is responsible to produce blood specifically the lymphatic system and bone marrow. Due to the harsh effects of currently used cancer drugs, damnacanthal, an anthraquinone obtained from the roots of Morinda elliptica is tested as a potential anticancer agent. This study reports on the participation of the p53, Bcl-2 and Bax in the apoptosis induced by of damnacanthal, on T-lymphoblastic leukaemia (CEM-SS) cell. Cell viability and morphology was tested with trypan blue assay, flow cytometry analysis detected the apoptotic activity of damnacanthal, caspase colorimetric protease assay tested the Caspase 2, 3, 6, 8, and 9’s involvement and Enzyme-linked Immunosorbent Assay (ELISA) was carried out to quantify the Human p53, Bcl-2, and Bax expression levels. Damnacanthal exhibited cytotoxicity at doses 10 and 30 μg/mL after 72 h of incubation. This study reports that damnacanthal arrested the cell at G2/M phase and initiates the apoptotic activity in the cells treated with 30 μg/mL of damnacanthal for 72 h through caspase 2 and 6 activation and not caspases 3, 8, and 9. Furthermore, this anthraquinone induces apoptosis via p53-independent pathway. Damnacanthal also lowered Bcl-2 and increased Bax activity in CEM-SS cell lines. These anticancer properties of damnacanthal makes it a potential agent to treat T-lymphoblastic leukaemia. |
| first_indexed | 2024-12-09T02:09:02Z |
| format | Article |
| id | ukm.eprints-24502 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-12-09T02:09:02Z |
| publishDate | 2024 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-245022024-11-12T07:02:28Z http://journalarticle.ukm.my/24502/ Mechanism of damnacanthal induced apoptosis in CEM-SS cell line Banulata Gopalsamy, Saiful Yazan Latifah, Hisyam Abdul Hamid, Leukaemia, is cancer of organs that is responsible to produce blood specifically the lymphatic system and bone marrow. Due to the harsh effects of currently used cancer drugs, damnacanthal, an anthraquinone obtained from the roots of Morinda elliptica is tested as a potential anticancer agent. This study reports on the participation of the p53, Bcl-2 and Bax in the apoptosis induced by of damnacanthal, on T-lymphoblastic leukaemia (CEM-SS) cell. Cell viability and morphology was tested with trypan blue assay, flow cytometry analysis detected the apoptotic activity of damnacanthal, caspase colorimetric protease assay tested the Caspase 2, 3, 6, 8, and 9’s involvement and Enzyme-linked Immunosorbent Assay (ELISA) was carried out to quantify the Human p53, Bcl-2, and Bax expression levels. Damnacanthal exhibited cytotoxicity at doses 10 and 30 μg/mL after 72 h of incubation. This study reports that damnacanthal arrested the cell at G2/M phase and initiates the apoptotic activity in the cells treated with 30 μg/mL of damnacanthal for 72 h through caspase 2 and 6 activation and not caspases 3, 8, and 9. Furthermore, this anthraquinone induces apoptosis via p53-independent pathway. Damnacanthal also lowered Bcl-2 and increased Bax activity in CEM-SS cell lines. These anticancer properties of damnacanthal makes it a potential agent to treat T-lymphoblastic leukaemia. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24502/1/SS%2020.pdf Banulata Gopalsamy, and Saiful Yazan Latifah, and Hisyam Abdul Hamid, (2024) Mechanism of damnacanthal induced apoptosis in CEM-SS cell line. Sains Malaysiana, 53 (9). pp. 3159-3171. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num9_2024/contentsVol53num9_2024.html |
| spellingShingle | Banulata Gopalsamy, Saiful Yazan Latifah, Hisyam Abdul Hamid, Mechanism of damnacanthal induced apoptosis in CEM-SS cell line |
| title | Mechanism of damnacanthal induced apoptosis in CEM-SS cell line |
| title_full | Mechanism of damnacanthal induced apoptosis in CEM-SS cell line |
| title_fullStr | Mechanism of damnacanthal induced apoptosis in CEM-SS cell line |
| title_full_unstemmed | Mechanism of damnacanthal induced apoptosis in CEM-SS cell line |
| title_short | Mechanism of damnacanthal induced apoptosis in CEM-SS cell line |
| title_sort | mechanism of damnacanthal induced apoptosis in cem ss cell line |
| url | http://journalarticle.ukm.my/24502/1/SS%2020.pdf |
| work_keys_str_mv | AT banulatagopalsamy mechanismofdamnacanthalinducedapoptosisincemsscellline AT saifulyazanlatifah mechanismofdamnacanthalinducedapoptosisincemsscellline AT hisyamabdulhamid mechanismofdamnacanthalinducedapoptosisincemsscellline |