Unveiling the cytotoxic and apoptosis-inducing abilities of an edible bird’s nest extract in human hepatocellular carcinoma Huh-7 cells
Edible bird’s nest (EBN) is renowned for diverse pharmacological properties; however, its ability to cause cancer cell death has not been found in recent studies. This study investigates the phytochemical composition of EBN ethanol extract (EEE), the cytotoxic and apoptosis-inducing effects of the e...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2024
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| Online Access: | http://journalarticle.ukm.my/24647/1/SS%2011.pdf |
| _version_ | 1824449786216972288 |
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| author | Nguyen, Thi-Phuong Duong, Tang Van Do, Hoang Dang Khoa Le, Van Minh Ta, Kim Nhung Nguyen, Khoa Thi |
| author_facet | Nguyen, Thi-Phuong Duong, Tang Van Do, Hoang Dang Khoa Le, Van Minh Ta, Kim Nhung Nguyen, Khoa Thi |
| author_sort | Nguyen, Thi-Phuong |
| collection | UKM |
| description | Edible bird’s nest (EBN) is renowned for diverse pharmacological properties; however, its ability to cause cancer cell death has not been found in recent studies. This study investigates the phytochemical composition of EBN ethanol extract (EEE), the cytotoxic and apoptosis-inducing effects of the extract on human hepatocellular carcinoma Huh-7 cells. Qualitative and quantitative tests showed that proteins and peptides were abundant in the EEE. In the MTT assay, the extract exhibited cytotoxic activity against Huh-7 cells (IC50 = 58.57 ± 2.66 μg/mL, 53.15 ± 3.07 μg/mL, and 55.27 ± 1.85 μg/mL at 24 h, 48 h, and 72 h of incubation, respectively). Annexin-V/PI staining suggested that the extract provoked apoptosis in Huh-7 cells by deregulating the expression patterns of apoptotic markers including caspase 3, caspase 8, caspase 9, and survivin. These findings demonstrate the potential of the EEE as a promising anticancer candidate for further studies. |
| first_indexed | 2025-02-19T02:08:22Z |
| format | Article |
| id | ukm.eprints-24647 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2025-02-19T02:08:22Z |
| publishDate | 2024 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-246472025-01-06T04:44:23Z http://journalarticle.ukm.my/24647/ Unveiling the cytotoxic and apoptosis-inducing abilities of an edible bird’s nest extract in human hepatocellular carcinoma Huh-7 cells Nguyen, Thi-Phuong Duong, Tang Van Do, Hoang Dang Khoa Le, Van Minh Ta, Kim Nhung Nguyen, Khoa Thi Edible bird’s nest (EBN) is renowned for diverse pharmacological properties; however, its ability to cause cancer cell death has not been found in recent studies. This study investigates the phytochemical composition of EBN ethanol extract (EEE), the cytotoxic and apoptosis-inducing effects of the extract on human hepatocellular carcinoma Huh-7 cells. Qualitative and quantitative tests showed that proteins and peptides were abundant in the EEE. In the MTT assay, the extract exhibited cytotoxic activity against Huh-7 cells (IC50 = 58.57 ± 2.66 μg/mL, 53.15 ± 3.07 μg/mL, and 55.27 ± 1.85 μg/mL at 24 h, 48 h, and 72 h of incubation, respectively). Annexin-V/PI staining suggested that the extract provoked apoptosis in Huh-7 cells by deregulating the expression patterns of apoptotic markers including caspase 3, caspase 8, caspase 9, and survivin. These findings demonstrate the potential of the EEE as a promising anticancer candidate for further studies. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24647/1/SS%2011.pdf Nguyen, Thi-Phuong and Duong, Tang Van and Do, Hoang Dang Khoa and Le, Van Minh and Ta, Kim Nhung and Nguyen, Khoa Thi (2024) Unveiling the cytotoxic and apoptosis-inducing abilities of an edible bird’s nest extract in human hepatocellular carcinoma Huh-7 cells. Sains Malaysiana, 53 (11). pp. 3673-3682. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num11_2024/contentsVol53num11_2024.html |
| spellingShingle | Nguyen, Thi-Phuong Duong, Tang Van Do, Hoang Dang Khoa Le, Van Minh Ta, Kim Nhung Nguyen, Khoa Thi Unveiling the cytotoxic and apoptosis-inducing abilities of an edible bird’s nest extract in human hepatocellular carcinoma Huh-7 cells |
| title | Unveiling the cytotoxic and apoptosis-inducing abilities of an edible bird’s nest extract in human hepatocellular carcinoma Huh-7 cells |
| title_full | Unveiling the cytotoxic and apoptosis-inducing abilities of an edible bird’s nest extract in human hepatocellular carcinoma Huh-7 cells |
| title_fullStr | Unveiling the cytotoxic and apoptosis-inducing abilities of an edible bird’s nest extract in human hepatocellular carcinoma Huh-7 cells |
| title_full_unstemmed | Unveiling the cytotoxic and apoptosis-inducing abilities of an edible bird’s nest extract in human hepatocellular carcinoma Huh-7 cells |
| title_short | Unveiling the cytotoxic and apoptosis-inducing abilities of an edible bird’s nest extract in human hepatocellular carcinoma Huh-7 cells |
| title_sort | unveiling the cytotoxic and apoptosis inducing abilities of an edible bird s nest extract in human hepatocellular carcinoma huh 7 cells |
| url | http://journalarticle.ukm.my/24647/1/SS%2011.pdf |
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