Antioxidant activity of Ardisia Crispa (Mata pelanduk)

The leaf and fruit crude extracts of hexane, chloroform, methanol and water of Ardisia crispa were screened for their antioxidant activity using DPPH radical scavenging, ferric reducing power, and metal chelating antioxidant assay. The methanol crude extract of fruits showed higher antioxidant activ...

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Main Authors: Hassan Mahmood Kzar Jindal, Jamaludin Mohamad
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2012
Online Access:http://journalarticle.ukm.my/4465/1/03%2520Hassan.pdf
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author Hassan Mahmood Kzar Jindal,
Jamaludin Mohamad,
author_facet Hassan Mahmood Kzar Jindal,
Jamaludin Mohamad,
author_sort Hassan Mahmood Kzar Jindal,
collection UKM
description The leaf and fruit crude extracts of hexane, chloroform, methanol and water of Ardisia crispa were screened for their antioxidant activity using DPPH radical scavenging, ferric reducing power, and metal chelating antioxidant assay. The methanol crude extract of fruits showed higher antioxidant activity (90.16 ± 0.01%) than the methanol crude extract of leaves (82.24 ± 0.02%) in the DPPH radical scavenging assay. In the ferric reducing power assay methanol fruit extract showed the highest absorbance indicating high antioxidant activities than leaf extract. In the metal chelating antioxidant assay fruit methanol extract gave 40% antioxidant activities than the leaf. Thin Layer Chromatography of the fruit methanol crude extract showed that it contained phenolic compounds when it was detected with folin reagent. HPLC analysis revealed that the fruit methanol extract contained gallic acid. This indicated that the high antioxidant activities of the fruits were due to the presence of gallic acid in the fruits of Ardisia crispa.
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spelling ukm.eprints-44652016-12-14T06:36:06Z http://journalarticle.ukm.my/4465/ Antioxidant activity of Ardisia Crispa (Mata pelanduk) Hassan Mahmood Kzar Jindal, Jamaludin Mohamad, The leaf and fruit crude extracts of hexane, chloroform, methanol and water of Ardisia crispa were screened for their antioxidant activity using DPPH radical scavenging, ferric reducing power, and metal chelating antioxidant assay. The methanol crude extract of fruits showed higher antioxidant activity (90.16 ± 0.01%) than the methanol crude extract of leaves (82.24 ± 0.02%) in the DPPH radical scavenging assay. In the ferric reducing power assay methanol fruit extract showed the highest absorbance indicating high antioxidant activities than leaf extract. In the metal chelating antioxidant assay fruit methanol extract gave 40% antioxidant activities than the leaf. Thin Layer Chromatography of the fruit methanol crude extract showed that it contained phenolic compounds when it was detected with folin reagent. HPLC analysis revealed that the fruit methanol extract contained gallic acid. This indicated that the high antioxidant activities of the fruits were due to the presence of gallic acid in the fruits of Ardisia crispa. Universiti Kebangsaan Malaysia 2012-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/4465/1/03%2520Hassan.pdf Hassan Mahmood Kzar Jindal, and Jamaludin Mohamad, (2012) Antioxidant activity of Ardisia Crispa (Mata pelanduk). Sains Malaysiana, 41 (5). pp. 539-545. ISSN 0126-6039 http://www.ukm.my/jsm/
spellingShingle Hassan Mahmood Kzar Jindal,
Jamaludin Mohamad,
Antioxidant activity of Ardisia Crispa (Mata pelanduk)
title Antioxidant activity of Ardisia Crispa (Mata pelanduk)
title_full Antioxidant activity of Ardisia Crispa (Mata pelanduk)
title_fullStr Antioxidant activity of Ardisia Crispa (Mata pelanduk)
title_full_unstemmed Antioxidant activity of Ardisia Crispa (Mata pelanduk)
title_short Antioxidant activity of Ardisia Crispa (Mata pelanduk)
title_sort antioxidant activity of ardisia crispa mata pelanduk
url http://journalarticle.ukm.my/4465/1/03%2520Hassan.pdf
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AT jamaludinmohamad antioxidantactivityofardisiacrispamatapelanduk