Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver
Gynura procumbens, a medicinal plant locally known as Sambung nyawa, is widely used traditionally in South East Asia as a remedy to alleviate symptoms of various ailments including diabetes mellitus. Previous investigators reported that the ethanol plant extract was hypoglycemic in streptozotocin (S...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universiti Kebangsaan Malaysia
2012
|
| Online Access: | http://journalarticle.ukm.my/5418/1/05%2520Chong%2520Ching.pdf |
| _version_ | 1825725545064693760 |
|---|---|
| author | Chong, Ching June Lee, Hui Wen Halimah Abdullah Sani, Jalifah Latip, Jualang Azlan Gansau, Lee, Ping Chin Noor Embi, Hasidah Mohd Sidek, |
| author_facet | Chong, Ching June Lee, Hui Wen Halimah Abdullah Sani, Jalifah Latip, Jualang Azlan Gansau, Lee, Ping Chin Noor Embi, Hasidah Mohd Sidek, |
| author_sort | Chong, Ching June |
| collection | UKM |
| description | Gynura procumbens, a medicinal plant locally known as Sambung nyawa, is widely used traditionally in South East Asia as a remedy to alleviate symptoms of various ailments including diabetes mellitus. Previous investigators reported that the ethanol plant extract was hypoglycemic in streptozotocin (STZ)-induced diabetic rats and suggested the blood glucose-reducing effect could be due to G. procumbens mimicking insulin effects. Our present study aimed to reevaluate the hypoglycemic effect(s) of G. procumbens and to ascertain the involvement of glycogen synthase kinase (GSK3), a key component of insulin biosignaling in the plant activity. A cell-based assay showed that the three G. procumbens fractions tested did not exhibit anti-GSK3 activities. Oral administrations of hexane, ethyl acetate, n-butanol fractions of G. procumbens (250 mg/kg b.w.) and Glibenclamide (5 mg/kg b.w.) for 14 days in STZ-induced diabetic rats reduced blood glucose levels by 29.7%, 60.1%, 33.5% and 61.7% (p<0.05), respectively compared to diabetic control. Liver glycogen contents were elevated (p<0.05), following administration of the three fractions of G. procumbens in diabetic rats when analysed after 14 days of treatment. Western blot analysis also showed GSK3β in the liver of G. procumbens fractions-treated and Glibenclamide-treated animals were phosphorylated at Ser-9. The above findings indicated that the hypoglycemic action of G. procumbens fractions resulted in the inactivation of GSK3β in liver of diabetic rats. Since G. procumbens fractions did not exhibit GSK3 inhibitory properties, it is possible that the hypoglycemic action of the fractions observed here could be due to direct or indirect effects on upstream components of the insulin biosignaling pathway. |
| first_indexed | 2024-03-06T03:57:08Z |
| format | Article |
| id | ukm.eprints-5418 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T03:57:08Z |
| publishDate | 2012 |
| publisher | Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-54182016-12-14T06:38:23Z http://journalarticle.ukm.my/5418/ Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver Chong, Ching June Lee, Hui Wen Halimah Abdullah Sani, Jalifah Latip, Jualang Azlan Gansau, Lee, Ping Chin Noor Embi, Hasidah Mohd Sidek, Gynura procumbens, a medicinal plant locally known as Sambung nyawa, is widely used traditionally in South East Asia as a remedy to alleviate symptoms of various ailments including diabetes mellitus. Previous investigators reported that the ethanol plant extract was hypoglycemic in streptozotocin (STZ)-induced diabetic rats and suggested the blood glucose-reducing effect could be due to G. procumbens mimicking insulin effects. Our present study aimed to reevaluate the hypoglycemic effect(s) of G. procumbens and to ascertain the involvement of glycogen synthase kinase (GSK3), a key component of insulin biosignaling in the plant activity. A cell-based assay showed that the three G. procumbens fractions tested did not exhibit anti-GSK3 activities. Oral administrations of hexane, ethyl acetate, n-butanol fractions of G. procumbens (250 mg/kg b.w.) and Glibenclamide (5 mg/kg b.w.) for 14 days in STZ-induced diabetic rats reduced blood glucose levels by 29.7%, 60.1%, 33.5% and 61.7% (p<0.05), respectively compared to diabetic control. Liver glycogen contents were elevated (p<0.05), following administration of the three fractions of G. procumbens in diabetic rats when analysed after 14 days of treatment. Western blot analysis also showed GSK3β in the liver of G. procumbens fractions-treated and Glibenclamide-treated animals were phosphorylated at Ser-9. The above findings indicated that the hypoglycemic action of G. procumbens fractions resulted in the inactivation of GSK3β in liver of diabetic rats. Since G. procumbens fractions did not exhibit GSK3 inhibitory properties, it is possible that the hypoglycemic action of the fractions observed here could be due to direct or indirect effects on upstream components of the insulin biosignaling pathway. Universiti Kebangsaan Malaysia 2012-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/5418/1/05%2520Chong%2520Ching.pdf Chong, Ching June and Lee, Hui Wen and Halimah Abdullah Sani, and Jalifah Latip, and Jualang Azlan Gansau, and Lee, Ping Chin and Noor Embi, and Hasidah Mohd Sidek, (2012) Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver. Sains Malaysiana, 41 (8). pp. 969-975. ISSN 0126-6039 http://www.ukm.my/jsm/ |
| spellingShingle | Chong, Ching June Lee, Hui Wen Halimah Abdullah Sani, Jalifah Latip, Jualang Azlan Gansau, Lee, Ping Chin Noor Embi, Hasidah Mohd Sidek, Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver |
| title | Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver |
| title_full | Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver |
| title_fullStr | Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver |
| title_full_unstemmed | Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver |
| title_short | Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver |
| title_sort | hypoglycemic effects of gynura procumbensfractions on streptozotocin induced diabetic rats involve phosphorylation of gsk3β ser 9 in liver |
| url | http://journalarticle.ukm.my/5418/1/05%2520Chong%2520Ching.pdf |
| work_keys_str_mv | AT chongchingjune hypoglycemiceffectsofgynuraprocumbensfractionsonstreptozotocininduceddiabeticratsinvolvephosphorylationofgsk3bser9inliver AT leehuiwen hypoglycemiceffectsofgynuraprocumbensfractionsonstreptozotocininduceddiabeticratsinvolvephosphorylationofgsk3bser9inliver AT halimahabdullahsani hypoglycemiceffectsofgynuraprocumbensfractionsonstreptozotocininduceddiabeticratsinvolvephosphorylationofgsk3bser9inliver AT jalifahlatip hypoglycemiceffectsofgynuraprocumbensfractionsonstreptozotocininduceddiabeticratsinvolvephosphorylationofgsk3bser9inliver AT jualangazlangansau hypoglycemiceffectsofgynuraprocumbensfractionsonstreptozotocininduceddiabeticratsinvolvephosphorylationofgsk3bser9inliver AT leepingchin hypoglycemiceffectsofgynuraprocumbensfractionsonstreptozotocininduceddiabeticratsinvolvephosphorylationofgsk3bser9inliver AT noorembi hypoglycemiceffectsofgynuraprocumbensfractionsonstreptozotocininduceddiabeticratsinvolvephosphorylationofgsk3bser9inliver AT hasidahmohdsidek hypoglycemiceffectsofgynuraprocumbensfractionsonstreptozotocininduceddiabeticratsinvolvephosphorylationofgsk3bser9inliver |