In vitro cytotoxic and antiproliferative effects of portulaca oleracea methanol extract on breast, cervical, colon and nasopharyngeal cancerous cell lines
Portulaca oleracea is a ubiquitous garden weed that has been traditionally used as antidiabetic and anti-inflammation agent. However, the potential anti-proliferative and cytotoxic effects of Portulaca oleracea towards cancerous cells are still unclear. Human hormone dependent breast cancer MCF-7 ce...
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| Format: | Article |
| Language: | English |
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Universiti Kebangsaan Malaysia
2013
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| Online Access: | http://journalarticle.ukm.my/6299/1/06_Gek_Choo_Sarah.pdf |
| _version_ | 1825725752204591104 |
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| author | Gek, Choo Sarah Tan Kar, Mun Wong Gui, Qi Pearle-Wong Siau, Li Yeo Swee, Keong Yeap Beow, Chin Yiap Hueh, Zan Chong |
| author_facet | Gek, Choo Sarah Tan Kar, Mun Wong Gui, Qi Pearle-Wong Siau, Li Yeo Swee, Keong Yeap Beow, Chin Yiap Hueh, Zan Chong |
| author_sort | Gek, Choo Sarah Tan |
| collection | UKM |
| description | Portulaca oleracea is a ubiquitous garden weed that has been traditionally used as antidiabetic and anti-inflammation agent. However, the potential anti-proliferative and cytotoxic effects of Portulaca oleracea towards cancerous cells are still unclear. Human hormone dependent breast cancer MCF-7 cell, colon cancer HT-29, cervical cancer Hela cell and nasopharyngeal cancer CNE-1 cell were used in this study. P. oleracea was extracted using methanol and the cytotoxicity against various cancerous cell lines was evaluated using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide solution (MTT) assay. The antiproliferation effect and cell cycle arrest were assessed using BrdU proliferation assay and flow cytometry cell cycle RNase/PI analysis, respectively. Portulaca oleracea methanol extract was able to reduce viability of all the tested cancerous cell lines. However, IC50 was only observed in CNE-1 cell (92 μg/mL). BrdU incorporation assay indicated anti-proliferation of Portulaca oleracea treated MCF-7 cells in a dose-dependent manner. A significant increase in the sub G0/G1cell population phase observed by cell cycle analysis indicates the occurrence of apoptotic events. In conclusion, Portulaca oleracea showed anti-proliferative effect on CNE-1, HeLa and HT-29 and DNA fragmentation on MCF-7 cells. |
| first_indexed | 2024-03-06T04:00:19Z |
| format | Article |
| id | ukm.eprints-6299 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:00:19Z |
| publishDate | 2013 |
| publisher | Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-62992016-12-14T06:40:47Z http://journalarticle.ukm.my/6299/ In vitro cytotoxic and antiproliferative effects of portulaca oleracea methanol extract on breast, cervical, colon and nasopharyngeal cancerous cell lines Gek, Choo Sarah Tan Kar, Mun Wong Gui, Qi Pearle-Wong Siau, Li Yeo Swee, Keong Yeap Beow, Chin Yiap Hueh, Zan Chong Portulaca oleracea is a ubiquitous garden weed that has been traditionally used as antidiabetic and anti-inflammation agent. However, the potential anti-proliferative and cytotoxic effects of Portulaca oleracea towards cancerous cells are still unclear. Human hormone dependent breast cancer MCF-7 cell, colon cancer HT-29, cervical cancer Hela cell and nasopharyngeal cancer CNE-1 cell were used in this study. P. oleracea was extracted using methanol and the cytotoxicity against various cancerous cell lines was evaluated using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide solution (MTT) assay. The antiproliferation effect and cell cycle arrest were assessed using BrdU proliferation assay and flow cytometry cell cycle RNase/PI analysis, respectively. Portulaca oleracea methanol extract was able to reduce viability of all the tested cancerous cell lines. However, IC50 was only observed in CNE-1 cell (92 μg/mL). BrdU incorporation assay indicated anti-proliferation of Portulaca oleracea treated MCF-7 cells in a dose-dependent manner. A significant increase in the sub G0/G1cell population phase observed by cell cycle analysis indicates the occurrence of apoptotic events. In conclusion, Portulaca oleracea showed anti-proliferative effect on CNE-1, HeLa and HT-29 and DNA fragmentation on MCF-7 cells. Universiti Kebangsaan Malaysia 2013-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6299/1/06_Gek_Choo_Sarah.pdf Gek, Choo Sarah Tan and Kar, Mun Wong and Gui, Qi Pearle-Wong and Siau, Li Yeo and Swee, Keong Yeap and Beow, Chin Yiap and Hueh, Zan Chong (2013) In vitro cytotoxic and antiproliferative effects of portulaca oleracea methanol extract on breast, cervical, colon and nasopharyngeal cancerous cell lines. Sains Malaysiana, 42 (7). pp. 927-935. ISSN 0126-6039 http://www.ukm.my/jsm/ |
| spellingShingle | Gek, Choo Sarah Tan Kar, Mun Wong Gui, Qi Pearle-Wong Siau, Li Yeo Swee, Keong Yeap Beow, Chin Yiap Hueh, Zan Chong In vitro cytotoxic and antiproliferative effects of portulaca oleracea methanol extract on breast, cervical, colon and nasopharyngeal cancerous cell lines |
| title | In vitro cytotoxic and antiproliferative effects of portulaca oleracea methanol extract on breast, cervical, colon and nasopharyngeal cancerous cell lines |
| title_full | In vitro cytotoxic and antiproliferative effects of portulaca oleracea methanol extract on breast, cervical, colon and nasopharyngeal cancerous cell lines |
| title_fullStr | In vitro cytotoxic and antiproliferative effects of portulaca oleracea methanol extract on breast, cervical, colon and nasopharyngeal cancerous cell lines |
| title_full_unstemmed | In vitro cytotoxic and antiproliferative effects of portulaca oleracea methanol extract on breast, cervical, colon and nasopharyngeal cancerous cell lines |
| title_short | In vitro cytotoxic and antiproliferative effects of portulaca oleracea methanol extract on breast, cervical, colon and nasopharyngeal cancerous cell lines |
| title_sort | in vitro cytotoxic and antiproliferative effects of portulaca oleracea methanol extract on breast cervical colon and nasopharyngeal cancerous cell lines |
| url | http://journalarticle.ukm.my/6299/1/06_Gek_Choo_Sarah.pdf |
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