Targeted RNAi of the mitogen-activated protein kinase pathway genes in acute myeloid leukemia cells
In this study, RNA interference (RNAi) was carried out as an experimental technique to knockdown three mitogen-activated protein kinase (MAPK) pathway genes, raf-1, mekk1 and mlk3 in acute myeloid leukemia (AML) cells. Conventionally, RNAi knockdown experiments target a single gene for functional st...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universiti Kebangsaan Malaysia
2013
|
| Online Access: | http://journalarticle.ukm.my/6452/1/13_M.R._Mohd_Hafiz.pdf |
| _version_ | 1796930297506299904 |
|---|---|
| author | Mohd Hafiz, M.R. Mazatulikhma, M.Z. Mohd Faiz, F.A. Mohamed Saifulaman, M.S. |
| author_facet | Mohd Hafiz, M.R. Mazatulikhma, M.Z. Mohd Faiz, F.A. Mohamed Saifulaman, M.S. |
| author_sort | Mohd Hafiz, M.R. |
| collection | UKM |
| description | In this study, RNA interference (RNAi) was carried out as an experimental technique to knockdown three mitogen-activated protein kinase (MAPK) pathway genes, raf-1, mekk1 and mlk3 in acute myeloid leukemia (AML) cells. Conventionally, RNAi knockdown experiments target a single gene for functional studies or therapeutic purposes. We wanted to explore the potential differences or similarities between targeting single targets or multiple target genes in a single application. We achieved knockdown of gene expression levels of between 40 and 60% for the RNAi experiments, with better knockdown observed in single target gene experiments in comparison with the multiple target gene experiment. Microarray analysis indicated that the transfection process had most likely induced the immune response from the cells in every RNAi treatment. This might indicate that when the MAPK signaling pathway is partially blocked, in tandem with the immune response, the cells will begin signaling for apoptosis leading to cellular death of the leukemic cells. |
| first_indexed | 2024-03-06T04:00:55Z |
| format | Article |
| id | ukm.eprints-6452 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:00:55Z |
| publishDate | 2013 |
| publisher | Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-64522016-12-14T06:41:13Z http://journalarticle.ukm.my/6452/ Targeted RNAi of the mitogen-activated protein kinase pathway genes in acute myeloid leukemia cells Mohd Hafiz, M.R. Mazatulikhma, M.Z. Mohd Faiz, F.A. Mohamed Saifulaman, M.S. In this study, RNA interference (RNAi) was carried out as an experimental technique to knockdown three mitogen-activated protein kinase (MAPK) pathway genes, raf-1, mekk1 and mlk3 in acute myeloid leukemia (AML) cells. Conventionally, RNAi knockdown experiments target a single gene for functional studies or therapeutic purposes. We wanted to explore the potential differences or similarities between targeting single targets or multiple target genes in a single application. We achieved knockdown of gene expression levels of between 40 and 60% for the RNAi experiments, with better knockdown observed in single target gene experiments in comparison with the multiple target gene experiment. Microarray analysis indicated that the transfection process had most likely induced the immune response from the cells in every RNAi treatment. This might indicate that when the MAPK signaling pathway is partially blocked, in tandem with the immune response, the cells will begin signaling for apoptosis leading to cellular death of the leukemic cells. Universiti Kebangsaan Malaysia 2013-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6452/1/13_M.R._Mohd_Hafiz.pdf Mohd Hafiz, M.R. and Mazatulikhma, M.Z. and Mohd Faiz, F.A. and Mohamed Saifulaman, M.S. (2013) Targeted RNAi of the mitogen-activated protein kinase pathway genes in acute myeloid leukemia cells. Sains Malaysiana, 42 (8). pp. 1131-1137. ISSN 0126-6039 http://www.ukm.my/jsm/ |
| spellingShingle | Mohd Hafiz, M.R. Mazatulikhma, M.Z. Mohd Faiz, F.A. Mohamed Saifulaman, M.S. Targeted RNAi of the mitogen-activated protein kinase pathway genes in acute myeloid leukemia cells |
| title | Targeted RNAi of the mitogen-activated protein kinase pathway
genes in acute myeloid leukemia cells |
| title_full | Targeted RNAi of the mitogen-activated protein kinase pathway
genes in acute myeloid leukemia cells |
| title_fullStr | Targeted RNAi of the mitogen-activated protein kinase pathway
genes in acute myeloid leukemia cells |
| title_full_unstemmed | Targeted RNAi of the mitogen-activated protein kinase pathway
genes in acute myeloid leukemia cells |
| title_short | Targeted RNAi of the mitogen-activated protein kinase pathway
genes in acute myeloid leukemia cells |
| title_sort | targeted rnai of the mitogen activated protein kinase pathway genes in acute myeloid leukemia cells |
| url | http://journalarticle.ukm.my/6452/1/13_M.R._Mohd_Hafiz.pdf |
| work_keys_str_mv | AT mohdhafizmr targetedrnaiofthemitogenactivatedproteinkinasepathwaygenesinacutemyeloidleukemiacells AT mazatulikhmamz targetedrnaiofthemitogenactivatedproteinkinasepathwaygenesinacutemyeloidleukemiacells AT mohdfaizfa targetedrnaiofthemitogenactivatedproteinkinasepathwaygenesinacutemyeloidleukemiacells AT mohamedsaifulamanms targetedrnaiofthemitogenactivatedproteinkinasepathwaygenesinacutemyeloidleukemiacells |