Process development for the production of protein hydrolysate from cockle (Anadara granosa) meat wash water

A study was carried out to determine the process parameters and optimization for the hydrolysis of protein precipitate from cockle (Anadara granosa) meat wash water. Precipitation of the protein in the wash water was done using pH manipulation (pH3-8). The precipitate was hydrolyzed using hydrochlor...

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Main Authors: Haslaniza, H., Maskat, M.Y., Wan Aida, W.M., Mamot, S.
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2014
Online Access:http://journalarticle.ukm.my/6815/1/07_H._Haslaniza.pdf
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author Haslaniza, H.
Maskat, M.Y.
Wan Aida, W.M.
Mamot, S.
author_facet Haslaniza, H.
Maskat, M.Y.
Wan Aida, W.M.
Mamot, S.
author_sort Haslaniza, H.
collection UKM
description A study was carried out to determine the process parameters and optimization for the hydrolysis of protein precipitate from cockle (Anadara granosa) meat wash water. Precipitation of the protein in the wash water was done using pH manipulation (pH3-8). The precipitate was hydrolyzed using hydrochloric acid (HCl) and optimized for HCl volume, HCl concentration and hydrolysis time using response surface methodology (RSM) based on a central composite rotatable design. Based on the results, hydrolysis of cockle meat wash water precipitate was carried out by precipitation of the wash water at pH4. Optimum condition for the hydrolysis of 2.0 g of cockle meat wash water precipitate was 25 mL of 1 N HCl for 10 h which resulted in nitrogen content (NC) of 0.7% and degree of hydrolysis (DH) of 55%. NC and DH were significantly influenced only by the hydrolysis time.
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spelling ukm.eprints-68152016-12-14T06:42:16Z http://journalarticle.ukm.my/6815/ Process development for the production of protein hydrolysate from cockle (Anadara granosa) meat wash water Haslaniza, H. Maskat, M.Y. Wan Aida, W.M. Mamot, S. A study was carried out to determine the process parameters and optimization for the hydrolysis of protein precipitate from cockle (Anadara granosa) meat wash water. Precipitation of the protein in the wash water was done using pH manipulation (pH3-8). The precipitate was hydrolyzed using hydrochloric acid (HCl) and optimized for HCl volume, HCl concentration and hydrolysis time using response surface methodology (RSM) based on a central composite rotatable design. Based on the results, hydrolysis of cockle meat wash water precipitate was carried out by precipitation of the wash water at pH4. Optimum condition for the hydrolysis of 2.0 g of cockle meat wash water precipitate was 25 mL of 1 N HCl for 10 h which resulted in nitrogen content (NC) of 0.7% and degree of hydrolysis (DH) of 55%. NC and DH were significantly influenced only by the hydrolysis time. Universiti Kebangsaan Malaysia 2014-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6815/1/07_H._Haslaniza.pdf Haslaniza, H. and Maskat, M.Y. and Wan Aida, W.M. and Mamot, S. (2014) Process development for the production of protein hydrolysate from cockle (Anadara granosa) meat wash water. Sains Malaysiana, 43 (1). pp. 53-63. ISSN 0126-6039 http://www.ukm.my/jsm/
spellingShingle Haslaniza, H.
Maskat, M.Y.
Wan Aida, W.M.
Mamot, S.
Process development for the production of protein hydrolysate from cockle (Anadara granosa) meat wash water
title Process development for the production of protein hydrolysate from cockle (Anadara granosa) meat wash water
title_full Process development for the production of protein hydrolysate from cockle (Anadara granosa) meat wash water
title_fullStr Process development for the production of protein hydrolysate from cockle (Anadara granosa) meat wash water
title_full_unstemmed Process development for the production of protein hydrolysate from cockle (Anadara granosa) meat wash water
title_short Process development for the production of protein hydrolysate from cockle (Anadara granosa) meat wash water
title_sort process development for the production of protein hydrolysate from cockle anadara granosa meat wash water
url http://journalarticle.ukm.my/6815/1/07_H._Haslaniza.pdf
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