Antimicrobial activities of aqueous lysate of Acanthamoeba spp. against selected pathogenic bacteria
The increasing of infectious diseases and emerging of multi-drug resistant bacteria are worldwide problems that require a search for a new potential drug from various sources including free-living amoebae to overcome these problems. In this study, the aqueous lysates of two isolates of Acanthamoeb...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2012
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| Online Access: | http://journalarticle.ukm.my/7459/1/41_2_7.pdf |
| _version_ | 1825726037093253120 |
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| author | Nakisah, M.A. Chandrika, K. |
| author_facet | Nakisah, M.A. Chandrika, K. |
| author_sort | Nakisah, M.A. |
| collection | UKM |
| description | The increasing of infectious diseases and emerging of multi-drug resistant bacteria are worldwide problems that require a
search for a new potential drug from various sources including free-living amoebae to overcome these problems. In this
study, the aqueous lysates of two isolates of Acanthamoeba viz A. castellanii, a clinical isolate designated as IMR isolate,
and Acanthamoeba sp., an environmental isolate designated as SW isolate were tested against two pathogenic bacteria, methicilin
resistant Staphylococcus aureus (MRSA) and Staphylococcus aureus. The anti-bacterial test was done using disc diffusion
(Kirby Bauer) method to determine the minimum inhibition concentration (MIC) values of the lysates against the bacteria.
Twofold dilution series of the Acanthamoeba lysates with concentrations ranging from 0.3 mg/mL to 2.4 mg/mL were used.
The measurements of diameter of inhibition zone of the amoeba lysates against each bacterium were compared with the
inhibition zone caused by a positive control (chloramphenicol) to analyse the effectiveness of the lysates as anti-bacterial
agents. MRSA and S. aureus tested were slightly sensitive to both lysates of Acanthamoeba used in this study to suggest the
potential of these lysates as bacterial agents. The data obtained were further confirmed by analysis of bacterial morphology
under SEM. |
| first_indexed | 2024-03-06T04:04:44Z |
| format | Article |
| id | ukm.eprints-7459 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:04:44Z |
| publishDate | 2012 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-74592016-12-14T06:44:09Z http://journalarticle.ukm.my/7459/ Antimicrobial activities of aqueous lysate of Acanthamoeba spp. against selected pathogenic bacteria Nakisah, M.A. Chandrika, K. The increasing of infectious diseases and emerging of multi-drug resistant bacteria are worldwide problems that require a search for a new potential drug from various sources including free-living amoebae to overcome these problems. In this study, the aqueous lysates of two isolates of Acanthamoeba viz A. castellanii, a clinical isolate designated as IMR isolate, and Acanthamoeba sp., an environmental isolate designated as SW isolate were tested against two pathogenic bacteria, methicilin resistant Staphylococcus aureus (MRSA) and Staphylococcus aureus. The anti-bacterial test was done using disc diffusion (Kirby Bauer) method to determine the minimum inhibition concentration (MIC) values of the lysates against the bacteria. Twofold dilution series of the Acanthamoeba lysates with concentrations ranging from 0.3 mg/mL to 2.4 mg/mL were used. The measurements of diameter of inhibition zone of the amoeba lysates against each bacterium were compared with the inhibition zone caused by a positive control (chloramphenicol) to analyse the effectiveness of the lysates as anti-bacterial agents. MRSA and S. aureus tested were slightly sensitive to both lysates of Acanthamoeba used in this study to suggest the potential of these lysates as bacterial agents. The data obtained were further confirmed by analysis of bacterial morphology under SEM. Penerbit Universiti Kebangsaan Malaysia 2012-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7459/1/41_2_7.pdf Nakisah, M.A. and Chandrika, K. (2012) Antimicrobial activities of aqueous lysate of Acanthamoeba spp. against selected pathogenic bacteria. Malaysian Applied Biology, 41 (2). pp. 45-49. ISSN 0126-8643 http://mabjournal.com/ |
| spellingShingle | Nakisah, M.A. Chandrika, K. Antimicrobial activities of aqueous lysate of Acanthamoeba spp. against selected pathogenic bacteria |
| title | Antimicrobial activities of aqueous lysate of Acanthamoeba spp. against selected pathogenic bacteria |
| title_full | Antimicrobial activities of aqueous lysate of Acanthamoeba spp. against selected pathogenic bacteria |
| title_fullStr | Antimicrobial activities of aqueous lysate of Acanthamoeba spp. against selected pathogenic bacteria |
| title_full_unstemmed | Antimicrobial activities of aqueous lysate of Acanthamoeba spp. against selected pathogenic bacteria |
| title_short | Antimicrobial activities of aqueous lysate of Acanthamoeba spp. against selected pathogenic bacteria |
| title_sort | antimicrobial activities of aqueous lysate of acanthamoeba spp against selected pathogenic bacteria |
| url | http://journalarticle.ukm.my/7459/1/41_2_7.pdf |
| work_keys_str_mv | AT nakisahma antimicrobialactivitiesofaqueouslysateofacanthamoebasppagainstselectedpathogenicbacteria AT chandrikak antimicrobialactivitiesofaqueouslysateofacanthamoebasppagainstselectedpathogenicbacteria |