Desiccation tolerance in phaleria macrocarpa embryonic axes
Phaleria macrocarpa seeds are rapidly killed with desiccation to moisture content (MC) below 20%. Desiccation tolerance of their embryonic axes was studied for storage and germplasm conservation purposes. Embryonic axes were extracted aseptically from fresh seeds obtained from fully ripe fruits in a...
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| Format: | Article |
| Language: | English |
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Universiti Kebangsaan Malaysia
2014
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| Online Access: | http://journalarticle.ukm.my/7808/1/02_S.M._Ahmed_Asrity.pdf |
| _version_ | 1796930629272600576 |
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| author | Ahmed Asrity, S.M. Tsan, F.Y. Ding, P. Syed Aris, S.R. |
| author_facet | Ahmed Asrity, S.M. Tsan, F.Y. Ding, P. Syed Aris, S.R. |
| author_sort | Ahmed Asrity, S.M. |
| collection | UKM |
| description | Phaleria macrocarpa seeds are rapidly killed with desiccation to moisture content (MC) below 20%. Desiccation tolerance of their embryonic axes was studied for storage and germplasm conservation purposes. Embryonic axes were extracted aseptically from fresh seeds obtained from fully ripe fruits in a horizontal laminar air flow cabinet. They were then desiccated under aseptic condition for periods ranging from 0-8 h. For each desiccation treatment, embryonic axes were drawn randomly for the determination of MC according to ISTA, electrolyte leakage and proliferation on Murashige and Skoog (MS) media supplemented with 1 mg/l 6-benzylaminopurine (BAP) and 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The results obtained from this study indicated that the embryonic axes could tolerate dehydration down to 13.6% with desiccation for 8 h while retaining relatively high viability of 76.7%. This was supported by only gradual increment of electrolyte leakage with the desiccated embryonic axes. All non-desiccated embryonic axes with MC of 52.5% were capable to grow into normal plantlets in vitro but dehydration to MC of 36.0% and further down to 13.6% generally resulted in callus formation with up to 16.7% of the embryonic axes while at least 60.0% of the other embryonic axes were still capable to proliferate as normal plantlets in vitro. |
| first_indexed | 2024-03-06T04:05:55Z |
| format | Article |
| id | ukm.eprints-7808 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:05:55Z |
| publishDate | 2014 |
| publisher | Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-78082016-12-14T06:45:16Z http://journalarticle.ukm.my/7808/ Desiccation tolerance in phaleria macrocarpa embryonic axes Ahmed Asrity, S.M. Tsan, F.Y. Ding, P. Syed Aris, S.R. Phaleria macrocarpa seeds are rapidly killed with desiccation to moisture content (MC) below 20%. Desiccation tolerance of their embryonic axes was studied for storage and germplasm conservation purposes. Embryonic axes were extracted aseptically from fresh seeds obtained from fully ripe fruits in a horizontal laminar air flow cabinet. They were then desiccated under aseptic condition for periods ranging from 0-8 h. For each desiccation treatment, embryonic axes were drawn randomly for the determination of MC according to ISTA, electrolyte leakage and proliferation on Murashige and Skoog (MS) media supplemented with 1 mg/l 6-benzylaminopurine (BAP) and 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The results obtained from this study indicated that the embryonic axes could tolerate dehydration down to 13.6% with desiccation for 8 h while retaining relatively high viability of 76.7%. This was supported by only gradual increment of electrolyte leakage with the desiccated embryonic axes. All non-desiccated embryonic axes with MC of 52.5% were capable to grow into normal plantlets in vitro but dehydration to MC of 36.0% and further down to 13.6% generally resulted in callus formation with up to 16.7% of the embryonic axes while at least 60.0% of the other embryonic axes were still capable to proliferate as normal plantlets in vitro. Universiti Kebangsaan Malaysia 2014-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7808/1/02_S.M._Ahmed_Asrity.pdf Ahmed Asrity, S.M. and Tsan, F.Y. and Ding, P. and Syed Aris, S.R. (2014) Desiccation tolerance in phaleria macrocarpa embryonic axes. Sains Malaysiana, 43 (10). pp. 1471-1475. ISSN 0126-6039 http://www.ukm.my/jsm/ |
| spellingShingle | Ahmed Asrity, S.M. Tsan, F.Y. Ding, P. Syed Aris, S.R. Desiccation tolerance in phaleria macrocarpa embryonic axes |
| title | Desiccation tolerance in phaleria macrocarpa embryonic axes |
| title_full | Desiccation tolerance in phaleria macrocarpa embryonic axes |
| title_fullStr | Desiccation tolerance in phaleria macrocarpa embryonic axes |
| title_full_unstemmed | Desiccation tolerance in phaleria macrocarpa embryonic axes |
| title_short | Desiccation tolerance in phaleria macrocarpa embryonic axes |
| title_sort | desiccation tolerance in phaleria macrocarpa embryonic axes |
| url | http://journalarticle.ukm.my/7808/1/02_S.M._Ahmed_Asrity.pdf |
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