Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features
Colorectal cancer develops in a multi-step process and is associated with genetic alterations. Blocking apoptosis is a key factor to unlimited cell proliferation and immortalization. Survivin expression inhibits the programmed cell death process, apoptosis. This molecule could play a potential rol...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2014
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| Online Access: | http://journalarticle.ukm.my/8677/1/43_2_05.pdf |
| _version_ | 1796930822954024960 |
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| author | Said Saleh, King, Alfred Yin Lam Yik, Hong Ho |
| author_facet | Said Saleh, King, Alfred Yin Lam Yik, Hong Ho |
| author_sort | Said Saleh, |
| collection | UKM |
| description | Colorectal cancer develops in a multi-step process and is associated with genetic alterations. Blocking apoptosis is a key
factor to unlimited cell proliferation and immortalization. Survivin expression inhibits the programmed cell death process,
apoptosis. This molecule could play a potential role in cancer development. Survivin is an attractive target for clinical trials to
develop cancer treatment. RNA was prepared from colorectal tumor and adjacent non-tumor tissues and then transcribed to
complementary DNA. Human survivin mRNA levels were quantitatively measured by real time polymerase chain reaction
(PCR) in tumor and adjacent non-tumor tissues. Expression levels of survivin mRNA in adenocarcinomas were significantly
higher than in non-tumor mucosa (p < 0.0001). The expressions of survivin mRNA in adenocarcinomas were related to the
degree of differentiation (survivin; p=0.034). No difference was found with other clinicopathological features. These findings
indicate survivin role in colorectal carcinogenesis. Survivin could be used as a potential diagnostic and prognostic marker in
colorectal cancer. Successful inhibition of this molecule could lead to the development of a new drug for cancer therapy. |
| first_indexed | 2024-03-06T04:08:53Z |
| format | Article |
| id | ukm.eprints-8677 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:08:53Z |
| publishDate | 2014 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-86772016-12-14T06:47:51Z http://journalarticle.ukm.my/8677/ Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features Said Saleh, King, Alfred Yin Lam Yik, Hong Ho Colorectal cancer develops in a multi-step process and is associated with genetic alterations. Blocking apoptosis is a key factor to unlimited cell proliferation and immortalization. Survivin expression inhibits the programmed cell death process, apoptosis. This molecule could play a potential role in cancer development. Survivin is an attractive target for clinical trials to develop cancer treatment. RNA was prepared from colorectal tumor and adjacent non-tumor tissues and then transcribed to complementary DNA. Human survivin mRNA levels were quantitatively measured by real time polymerase chain reaction (PCR) in tumor and adjacent non-tumor tissues. Expression levels of survivin mRNA in adenocarcinomas were significantly higher than in non-tumor mucosa (p < 0.0001). The expressions of survivin mRNA in adenocarcinomas were related to the degree of differentiation (survivin; p=0.034). No difference was found with other clinicopathological features. These findings indicate survivin role in colorectal carcinogenesis. Survivin could be used as a potential diagnostic and prognostic marker in colorectal cancer. Successful inhibition of this molecule could lead to the development of a new drug for cancer therapy. Penerbit Universiti Kebangsaan Malaysia 2014-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8677/1/43_2_05.pdf Said Saleh, and King, Alfred Yin Lam and Yik, Hong Ho (2014) Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features. Malaysian Applied Biology, 43 (2). pp. 35-40. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=460&catid=59:current-view&Itemid=56 |
| spellingShingle | Said Saleh, King, Alfred Yin Lam Yik, Hong Ho Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features |
| title | Survivin expression in colorectal: real time
polymerase chain reaction and correlation with
clinicopathological features |
| title_full | Survivin expression in colorectal: real time
polymerase chain reaction and correlation with
clinicopathological features |
| title_fullStr | Survivin expression in colorectal: real time
polymerase chain reaction and correlation with
clinicopathological features |
| title_full_unstemmed | Survivin expression in colorectal: real time
polymerase chain reaction and correlation with
clinicopathological features |
| title_short | Survivin expression in colorectal: real time
polymerase chain reaction and correlation with
clinicopathological features |
| title_sort | survivin expression in colorectal real time polymerase chain reaction and correlation with clinicopathological features |
| url | http://journalarticle.ukm.my/8677/1/43_2_05.pdf |
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