Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time
Oil Palm Knotted-like 1(OPKN1) gene is a type of homeobox gene that was previously isolated from cDNA library of oil palm’s suspension cell culture. Based on semi-quantitative RT-PCR, northern blotting and in situ hybridization analysis, the OPKN1 gene is active in flower meristem suggesting its i...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2015
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| Online Access: | http://journalarticle.ukm.my/8715/1/44_1_17.pdf |
| _version_ | 1796930833995530240 |
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| author | Mohd Waznul Adly, M.Z. Zuraida, A.B. Wee, C.Y. Alizah, Z. Norliza, A.B. Che Radziah, C.M.Z. |
| author_facet | Mohd Waznul Adly, M.Z. Zuraida, A.B. Wee, C.Y. Alizah, Z. Norliza, A.B. Che Radziah, C.M.Z. |
| author_sort | Mohd Waznul Adly, M.Z. |
| collection | UKM |
| description | Oil Palm Knotted-like 1(OPKN1) gene is a type of homeobox gene that was previously isolated from cDNA library of oil
palm’s suspension cell culture. Based on semi-quantitative RT-PCR, northern blotting and in situ hybridization analysis, the
OPKN1 gene is active in flower meristem suggesting its involvement in flowering phase. Semi-quantitative RT-PCR analysis
indicated that OPKN1 gene is active in both male and female flowers and also in abnormal flower suggesting its role in
flower morphogenesis process. To further validate the function of this gene, the overexpression transformation vector of
OPKN1 gene driven by CaMV 35S constitutive promoter was constructed. The constructed vector was transformed into
plant model systems Nicotiana benthamiana via Agrobacterium tumefaciens. Four transgenics lines of N. benthamiana were
successfully obtained and analysis of quantitative Real-Time PCR shows that OPKN1 gene was highly overexpressed in all
transgenic lines. Overexpression of OPKN1 gene in N. benthamiana transformant has caused reduction in N. benthamiana’s
flower size and flowering time was also observed to be delayed. Based on this finding, it was suggested that OPKN1 may be
involved in flower development and morphogenesis process. |
| first_indexed | 2024-03-06T04:09:03Z |
| format | Article |
| id | ukm.eprints-8715 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:09:03Z |
| publishDate | 2015 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-87152016-12-14T06:47:59Z http://journalarticle.ukm.my/8715/ Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time Mohd Waznul Adly, M.Z. Zuraida, A.B. Wee, C.Y. Alizah, Z. Norliza, A.B. Che Radziah, C.M.Z. Oil Palm Knotted-like 1(OPKN1) gene is a type of homeobox gene that was previously isolated from cDNA library of oil palm’s suspension cell culture. Based on semi-quantitative RT-PCR, northern blotting and in situ hybridization analysis, the OPKN1 gene is active in flower meristem suggesting its involvement in flowering phase. Semi-quantitative RT-PCR analysis indicated that OPKN1 gene is active in both male and female flowers and also in abnormal flower suggesting its role in flower morphogenesis process. To further validate the function of this gene, the overexpression transformation vector of OPKN1 gene driven by CaMV 35S constitutive promoter was constructed. The constructed vector was transformed into plant model systems Nicotiana benthamiana via Agrobacterium tumefaciens. Four transgenics lines of N. benthamiana were successfully obtained and analysis of quantitative Real-Time PCR shows that OPKN1 gene was highly overexpressed in all transgenic lines. Overexpression of OPKN1 gene in N. benthamiana transformant has caused reduction in N. benthamiana’s flower size and flowering time was also observed to be delayed. Based on this finding, it was suggested that OPKN1 may be involved in flower development and morphogenesis process. Penerbit Universiti Kebangsaan Malaysia 2015-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8715/1/44_1_17.pdf Mohd Waznul Adly, M.Z. and Zuraida, A.B. and Wee, C.Y. and Alizah, Z. and Norliza, A.B. and Che Radziah, C.M.Z. (2015) Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time. Malaysian Applied Biology, 44 (1). pp. 101-106. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=505&catid=59:current-view&Itemid=56 |
| spellingShingle | Mohd Waznul Adly, M.Z. Zuraida, A.B. Wee, C.Y. Alizah, Z. Norliza, A.B. Che Radziah, C.M.Z. Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time |
| title | Ectopic expression of OPKN1 gene in N. benthamiana
alter flower size and delay flowering time |
| title_full | Ectopic expression of OPKN1 gene in N. benthamiana
alter flower size and delay flowering time |
| title_fullStr | Ectopic expression of OPKN1 gene in N. benthamiana
alter flower size and delay flowering time |
| title_full_unstemmed | Ectopic expression of OPKN1 gene in N. benthamiana
alter flower size and delay flowering time |
| title_short | Ectopic expression of OPKN1 gene in N. benthamiana
alter flower size and delay flowering time |
| title_sort | ectopic expression of opkn1 gene in n benthamiana alter flower size and delay flowering time |
| url | http://journalarticle.ukm.my/8715/1/44_1_17.pdf |
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