Molecular characterization of colletotrichum isolates associated with anthracnose of mango fruit
Colletotrichum species are well-known causal agent of anthracnose. A study was conducted to determine the identity of Colletotrichum associated with anthracnose of mango (Mangifera indica) fruits. Thirty five Colletotrichum isolates were isolated from anthracnose lesion of two mango cultivars, Choka...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universiti Kebangsaan Malaysia
2015
|
| Online Access: | http://journalarticle.ukm.my/8929/1/02_Latiffah_Zakaria.pdf |
| _version_ | 1796930874574372864 |
|---|---|
| author | Latiffah Zakaria, Nurul Zaadah Juhari, Suzianti Iskandar Vijaya, Intan Sakinah Mohd Anuar, |
| author_facet | Latiffah Zakaria, Nurul Zaadah Juhari, Suzianti Iskandar Vijaya, Intan Sakinah Mohd Anuar, |
| author_sort | Latiffah Zakaria, |
| collection | UKM |
| description | Colletotrichum species are well-known causal agent of anthracnose. A study was conducted to determine the identity of Colletotrichum associated with anthracnose of mango (Mangifera indica) fruits. Thirty five Colletotrichum isolates were isolated from anthracnose lesion of two mango cultivars, Chokanan and Harum Manis. Based on the conidial morphology, two morphotypes (I and II) of C. gleosporioides were identified. Based on BLAST search of ITS regions and β-tubulin sequences, majority of the isolates showed 99-100% similarity with Colletotrichum sp. from mango and other hosts and three isolates, 100% similarity with C. asianum. From phylogenetic analysis using maximum likelihood method of combined datasets, the isolates from mango formed three clades, which corresponded to C. gloeosporioides sensu lato and C. asianum. Therefore, the present study showed that the isolates associated with anthracnose of mango belong to C. gleosporioides sensu lato and C. asianum. |
| first_indexed | 2024-03-06T04:09:39Z |
| format | Article |
| id | ukm.eprints-8929 |
| institution | Universiti Kebangsaan Malaysia |
| language | English |
| last_indexed | 2024-03-06T04:09:39Z |
| publishDate | 2015 |
| publisher | Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | ukm.eprints-89292016-12-14T06:48:27Z http://journalarticle.ukm.my/8929/ Molecular characterization of colletotrichum isolates associated with anthracnose of mango fruit Latiffah Zakaria, Nurul Zaadah Juhari, Suzianti Iskandar Vijaya, Intan Sakinah Mohd Anuar, Colletotrichum species are well-known causal agent of anthracnose. A study was conducted to determine the identity of Colletotrichum associated with anthracnose of mango (Mangifera indica) fruits. Thirty five Colletotrichum isolates were isolated from anthracnose lesion of two mango cultivars, Chokanan and Harum Manis. Based on the conidial morphology, two morphotypes (I and II) of C. gleosporioides were identified. Based on BLAST search of ITS regions and β-tubulin sequences, majority of the isolates showed 99-100% similarity with Colletotrichum sp. from mango and other hosts and three isolates, 100% similarity with C. asianum. From phylogenetic analysis using maximum likelihood method of combined datasets, the isolates from mango formed three clades, which corresponded to C. gloeosporioides sensu lato and C. asianum. Therefore, the present study showed that the isolates associated with anthracnose of mango belong to C. gleosporioides sensu lato and C. asianum. Universiti Kebangsaan Malaysia 2015-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8929/1/02_Latiffah_Zakaria.pdf Latiffah Zakaria, and Nurul Zaadah Juhari, and Suzianti Iskandar Vijaya, and Intan Sakinah Mohd Anuar, (2015) Molecular characterization of colletotrichum isolates associated with anthracnose of mango fruit. Sains Malaysiana, 44 (5). pp. 651-656. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid44bil5_2015/KandunganJilid44Bil5_2015.html |
| spellingShingle | Latiffah Zakaria, Nurul Zaadah Juhari, Suzianti Iskandar Vijaya, Intan Sakinah Mohd Anuar, Molecular characterization of colletotrichum isolates associated with anthracnose of mango fruit |
| title | Molecular characterization of colletotrichum isolates associated
with anthracnose of mango fruit |
| title_full | Molecular characterization of colletotrichum isolates associated
with anthracnose of mango fruit |
| title_fullStr | Molecular characterization of colletotrichum isolates associated
with anthracnose of mango fruit |
| title_full_unstemmed | Molecular characterization of colletotrichum isolates associated
with anthracnose of mango fruit |
| title_short | Molecular characterization of colletotrichum isolates associated
with anthracnose of mango fruit |
| title_sort | molecular characterization of colletotrichum isolates associated with anthracnose of mango fruit |
| url | http://journalarticle.ukm.my/8929/1/02_Latiffah_Zakaria.pdf |
| work_keys_str_mv | AT latiffahzakaria molecularcharacterizationofcolletotrichumisolatesassociatedwithanthracnoseofmangofruit AT nurulzaadahjuhari molecularcharacterizationofcolletotrichumisolatesassociatedwithanthracnoseofmangofruit AT suziantiiskandarvijaya molecularcharacterizationofcolletotrichumisolatesassociatedwithanthracnoseofmangofruit AT intansakinahmohdanuar molecularcharacterizationofcolletotrichumisolatesassociatedwithanthracnoseofmangofruit |