Upregulation of insulin secretion and downregulation of pro-inflammatory cytokines, oxidative stress and hyperglycemia in STZ-nicotinamide-induced type 2 diabetic rats by Pseuduvaria monticola bark extract
The current study aimed to ascertain the antidiabetic potential of Pseuduvaria monticola bark methanolic extract (PMm) using in vitro mechanistic study models. In particular, the study determined the effect of PMm on cellular viability, 2-NBDG glucose uptake, insulin secretion, and NF-jB translocat...
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| Format: | Article |
| Language: | English English |
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Elsevier
2014
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| Online Access: | http://eprints.um.edu.my/10514/1/Upregulation_of_insulin_secretion_and_downregulation.pdf http://eprints.um.edu.my/10514/4/00011677_103500.pdf |
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| author | Taha, H. Arya, Aditya Paydar, M. Looi, Chung Yeng Wong, Won Fen Murthy, C.R.V. Noordin, M.I. Ali, Hapipah Mohd Mustafa, A.M. Hadi, A.H.A. |
| author_facet | Taha, H. Arya, Aditya Paydar, M. Looi, Chung Yeng Wong, Won Fen Murthy, C.R.V. Noordin, M.I. Ali, Hapipah Mohd Mustafa, A.M. Hadi, A.H.A. |
| author_sort | Taha, H. |
| collection | UM |
| description | The current study aimed to ascertain the antidiabetic potential of Pseuduvaria monticola bark methanolic
extract (PMm) using in vitro mechanistic study models. In particular, the study determined the effect of PMm on cellular viability, 2-NBDG glucose uptake, insulin secretion, and NF-jB translocation in mouse pancreatic insulinoma cells (NIT-1). Furthermore, in vivo acute toxicity and antidiabetic studies were performed
using streptozotocin (STZ)-induced type 1 and STZ-nicotinamide-induced type 2 diabetic rat models to evaluate various biochemical parameters and markers of oxidative stress and pro-inflammatory cytokines. Five isoquinoline alkaloids and three phenolic compounds were tentatively identified in the PMm by LC/MS Triple TOF. The study results showed that PMm is non-toxic to NIT-1 cells and significantly increased the glucose uptake and insulin secretion without affecting the translocation of NF-jB.
Moreover, the non-toxic effects of PMm were confirmed through an in vivo acute toxicity study, which revealed that the serum insulin and C-peptide levels were significantly upregulated in type 2 diabetic rats and that no significant changes were observed in type 1 diabetic rats. Similarly, PMm was found to downregulate the levels of oxidative stress and pro-inflammatory cytokines in type 2 diabetic rats by alleviating hyperglycemia. Therefore, we conclude that PMm may be developed as an antidiabetic agent for the treatment of type 2 diabetes-associated conditions. |
| first_indexed | 2024-03-06T05:26:20Z |
| format | Article |
| id | um.eprints-10514 |
| institution | Universiti Malaya |
| language | English English |
| last_indexed | 2024-03-06T05:26:20Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | um.eprints-105142019-04-03T06:38:16Z http://eprints.um.edu.my/10514/ Upregulation of insulin secretion and downregulation of pro-inflammatory cytokines, oxidative stress and hyperglycemia in STZ-nicotinamide-induced type 2 diabetic rats by Pseuduvaria monticola bark extract Taha, H. Arya, Aditya Paydar, M. Looi, Chung Yeng Wong, Won Fen Murthy, C.R.V. Noordin, M.I. Ali, Hapipah Mohd Mustafa, A.M. Hadi, A.H.A. Q Science (General) QD Chemistry R Medicine The current study aimed to ascertain the antidiabetic potential of Pseuduvaria monticola bark methanolic extract (PMm) using in vitro mechanistic study models. In particular, the study determined the effect of PMm on cellular viability, 2-NBDG glucose uptake, insulin secretion, and NF-jB translocation in mouse pancreatic insulinoma cells (NIT-1). Furthermore, in vivo acute toxicity and antidiabetic studies were performed using streptozotocin (STZ)-induced type 1 and STZ-nicotinamide-induced type 2 diabetic rat models to evaluate various biochemical parameters and markers of oxidative stress and pro-inflammatory cytokines. Five isoquinoline alkaloids and three phenolic compounds were tentatively identified in the PMm by LC/MS Triple TOF. The study results showed that PMm is non-toxic to NIT-1 cells and significantly increased the glucose uptake and insulin secretion without affecting the translocation of NF-jB. Moreover, the non-toxic effects of PMm were confirmed through an in vivo acute toxicity study, which revealed that the serum insulin and C-peptide levels were significantly upregulated in type 2 diabetic rats and that no significant changes were observed in type 1 diabetic rats. Similarly, PMm was found to downregulate the levels of oxidative stress and pro-inflammatory cytokines in type 2 diabetic rats by alleviating hyperglycemia. Therefore, we conclude that PMm may be developed as an antidiabetic agent for the treatment of type 2 diabetes-associated conditions. Elsevier 2014 Article PeerReviewed text en http://eprints.um.edu.my/10514/1/Upregulation_of_insulin_secretion_and_downregulation.pdf text en http://eprints.um.edu.my/10514/4/00011677_103500.pdf Taha, H. and Arya, Aditya and Paydar, M. and Looi, Chung Yeng and Wong, Won Fen and Murthy, C.R.V. and Noordin, M.I. and Ali, Hapipah Mohd and Mustafa, A.M. and Hadi, A.H.A. (2014) Upregulation of insulin secretion and downregulation of pro-inflammatory cytokines, oxidative stress and hyperglycemia in STZ-nicotinamide-induced type 2 diabetic rats by Pseuduvaria monticola bark extract. Food and Chemical Toxicology, 66. pp. 295-306. ISSN 0278-6915, DOI https://doi.org/10.1016/j.fct.2014.01.054 <https://doi.org/10.1016/j.fct.2014.01.054>. https://doi.org/10.1016/j.fct.2014.01.054 doi:10.1016/j.fct.2014.01.054 |
| spellingShingle | Q Science (General) QD Chemistry R Medicine Taha, H. Arya, Aditya Paydar, M. Looi, Chung Yeng Wong, Won Fen Murthy, C.R.V. Noordin, M.I. Ali, Hapipah Mohd Mustafa, A.M. Hadi, A.H.A. Upregulation of insulin secretion and downregulation of pro-inflammatory cytokines, oxidative stress and hyperglycemia in STZ-nicotinamide-induced type 2 diabetic rats by Pseuduvaria monticola bark extract |
| title | Upregulation of insulin secretion and downregulation of pro-inflammatory cytokines, oxidative stress and hyperglycemia in STZ-nicotinamide-induced type 2 diabetic rats by Pseuduvaria monticola bark extract |
| title_full | Upregulation of insulin secretion and downregulation of pro-inflammatory cytokines, oxidative stress and hyperglycemia in STZ-nicotinamide-induced type 2 diabetic rats by Pseuduvaria monticola bark extract |
| title_fullStr | Upregulation of insulin secretion and downregulation of pro-inflammatory cytokines, oxidative stress and hyperglycemia in STZ-nicotinamide-induced type 2 diabetic rats by Pseuduvaria monticola bark extract |
| title_full_unstemmed | Upregulation of insulin secretion and downregulation of pro-inflammatory cytokines, oxidative stress and hyperglycemia in STZ-nicotinamide-induced type 2 diabetic rats by Pseuduvaria monticola bark extract |
| title_short | Upregulation of insulin secretion and downregulation of pro-inflammatory cytokines, oxidative stress and hyperglycemia in STZ-nicotinamide-induced type 2 diabetic rats by Pseuduvaria monticola bark extract |
| title_sort | upregulation of insulin secretion and downregulation of pro inflammatory cytokines oxidative stress and hyperglycemia in stz nicotinamide induced type 2 diabetic rats by pseuduvaria monticola bark extract |
| topic | Q Science (General) QD Chemistry R Medicine |
| url | http://eprints.um.edu.my/10514/1/Upregulation_of_insulin_secretion_and_downregulation.pdf http://eprints.um.edu.my/10514/4/00011677_103500.pdf |
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