High diversity of Cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene
Background: Currently, there is a lack of vital information in the genetic makeup of Cryptosporidium especially in developing countries. The present study aimed at determining the genotypes and subgenotypes of Cryptosporidium in hospitalized Malaysian human immunodeficiency virus (HIV) positive pat...
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| Format: | Article |
| Language: | English |
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Public Library of Science
2012
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| Online Access: | http://eprints.um.edu.my/10547/1/High_Diversity_of_Cryptosporidium_Subgenotypes_Identified_in_Malaysian_HIV_AIDS_Individuals_Targeting_gp60_Gene.pdf |
| _version_ | 1825719876653678592 |
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| author | Iqbal, Asma Lim, Yvonne Ai Lian Surin, Johari Sim, Benedict Lim Heng |
| author_facet | Iqbal, Asma Lim, Yvonne Ai Lian Surin, Johari Sim, Benedict Lim Heng |
| author_sort | Iqbal, Asma |
| collection | UM |
| description | Background:
Currently, there is a lack of vital information in the genetic makeup of Cryptosporidium especially in developing countries. The present study aimed at determining the genotypes and subgenotypes of Cryptosporidium in hospitalized Malaysian human immunodeficiency virus (HIV) positive patients.
Methodology/Principal Findings:
In this study, 346 faecal samples collected from Malaysian HIV positive patients were genetically analysed via PCR targeting the 60 kDa glycoprotein (gp60) gene. Eighteen (5.2% of 346) isolates were determined as Cryptosporidium positive with 72.2% (of 18) identified as Cryptosporidium parvum whilst 27.7% as Cryptosporidium hominis. Further gp60 analysis revealed C. parvum belonging to subgenotypes IIaA13G1R1 (2 isolates), IIaA13G2R1 (2 isolates), IIaA14G2R1 (3 isolates), IIaA15G2R1 (5 isolates) and IIdA15G1R1 (1 isolate). C. hominis was represented by subgenotypes IaA14R1 (2 isolates), IaA18R1 (1 isolate) and IbA10G2R2 (2 isolates).
Conclusions/Significance:
These findings highlighted the presence of high diversity of Cryptosporidium subgenotypes among Malaysian HIV infected individuals. The predominance of the C. parvum subgenotypes signified the possibility of zoonotic as well as anthroponotic transmissions of cryptosporidiosis in HIV infected individuals. |
| first_indexed | 2024-03-06T05:26:25Z |
| format | Article |
| id | um.eprints-10547 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:26:25Z |
| publishDate | 2012 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | um.eprints-105472019-12-02T03:53:02Z http://eprints.um.edu.my/10547/ High diversity of Cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene Iqbal, Asma Lim, Yvonne Ai Lian Surin, Johari Sim, Benedict Lim Heng Q Science (General) R Medicine (General) Background: Currently, there is a lack of vital information in the genetic makeup of Cryptosporidium especially in developing countries. The present study aimed at determining the genotypes and subgenotypes of Cryptosporidium in hospitalized Malaysian human immunodeficiency virus (HIV) positive patients. Methodology/Principal Findings: In this study, 346 faecal samples collected from Malaysian HIV positive patients were genetically analysed via PCR targeting the 60 kDa glycoprotein (gp60) gene. Eighteen (5.2% of 346) isolates were determined as Cryptosporidium positive with 72.2% (of 18) identified as Cryptosporidium parvum whilst 27.7% as Cryptosporidium hominis. Further gp60 analysis revealed C. parvum belonging to subgenotypes IIaA13G1R1 (2 isolates), IIaA13G2R1 (2 isolates), IIaA14G2R1 (3 isolates), IIaA15G2R1 (5 isolates) and IIdA15G1R1 (1 isolate). C. hominis was represented by subgenotypes IaA14R1 (2 isolates), IaA18R1 (1 isolate) and IbA10G2R2 (2 isolates). Conclusions/Significance: These findings highlighted the presence of high diversity of Cryptosporidium subgenotypes among Malaysian HIV infected individuals. The predominance of the C. parvum subgenotypes signified the possibility of zoonotic as well as anthroponotic transmissions of cryptosporidiosis in HIV infected individuals. Public Library of Science 2012 Article PeerReviewed application/pdf en http://eprints.um.edu.my/10547/1/High_Diversity_of_Cryptosporidium_Subgenotypes_Identified_in_Malaysian_HIV_AIDS_Individuals_Targeting_gp60_Gene.pdf Iqbal, Asma and Lim, Yvonne Ai Lian and Surin, Johari and Sim, Benedict Lim Heng (2012) High diversity of Cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene. PLoS ONE, 7 (2). e31139. ISSN 1932-6203, DOI https://doi.org/10.1371/journal.pone.0031139 <https://doi.org/10.1371/journal.pone.0031139>. https://doi.org/10.1371/journal.pone.0031139 doi:10.1371/journal.pone.0031139 |
| spellingShingle | Q Science (General) R Medicine (General) Iqbal, Asma Lim, Yvonne Ai Lian Surin, Johari Sim, Benedict Lim Heng High diversity of Cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene |
| title | High diversity of Cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene |
| title_full | High diversity of Cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene |
| title_fullStr | High diversity of Cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene |
| title_full_unstemmed | High diversity of Cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene |
| title_short | High diversity of Cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene |
| title_sort | high diversity of cryptosporidium subgenotypes identified in malaysian hiv aids individuals targeting gp60 gene |
| topic | Q Science (General) R Medicine (General) |
| url | http://eprints.um.edu.my/10547/1/High_Diversity_of_Cryptosporidium_Subgenotypes_Identified_in_Malaysian_HIV_AIDS_Individuals_Targeting_gp60_Gene.pdf |
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