Evaluation of antiviral activities of four local malaysian phyllanthus species against herpes simplex viruses and possible antiviral target

Nucleoside analogues such as acyclovir are effective antiviral drugs against herpes simplex virus infections since its introduction. However, with the emergence of acyclovir-resistant HSV strains particularly in immunocompromised patients, there is a need to develop an alternative antiherpetic drug...

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Main Authors: Tan, W.C., Jaganath, I.B., Manikam, R., Sekaran, S.D.
Format: Article
Published: 2013
Subjects:
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author Tan, W.C.
Jaganath, I.B.
Manikam, R.
Sekaran, S.D.
author_facet Tan, W.C.
Jaganath, I.B.
Manikam, R.
Sekaran, S.D.
author_sort Tan, W.C.
collection UM
description Nucleoside analogues such as acyclovir are effective antiviral drugs against herpes simplex virus infections since its introduction. However, with the emergence of acyclovir-resistant HSV strains particularly in immunocompromised patients, there is a need to develop an alternative antiherpetic drug and plants could be the potential lead. In this study, the antiviral activity of the aqueous extract of four Phyllanthus species were evaluated against herpes simplex virus type-1 (HSV-1) and HSV-2 in Vero cells by quantitative PCR. The protein expressions of untreated and treated infected Vero cells were studied by 2D-gel electrophoresis and Western blot. This is the first study that reported the antiviral activity of P. watsonii. P. urinaria was shown to demonstrate the strongest antiviral activity against HSV-1 and HSV-2, with SI > 33.6. Time-of-addition studies suggested that the extract may act against the early infection stage and the replication stage. Protein expression studies indicated that cellular proteins that are involved in maintaining cytoskeletal structure could be potential target for development of antiviral drugs. Preliminary findings indicated that P. urinaria demonstrated potent inhibitory activity against HSV. Hence, further studies such as in vivo evaluation are required for the development of effective antiherpetic drug.
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spelling um.eprints-106252014-08-20T01:22:46Z http://eprints.um.edu.my/10625/ Evaluation of antiviral activities of four local malaysian phyllanthus species against herpes simplex viruses and possible antiviral target Tan, W.C. Jaganath, I.B. Manikam, R. Sekaran, S.D. R Medicine Nucleoside analogues such as acyclovir are effective antiviral drugs against herpes simplex virus infections since its introduction. However, with the emergence of acyclovir-resistant HSV strains particularly in immunocompromised patients, there is a need to develop an alternative antiherpetic drug and plants could be the potential lead. In this study, the antiviral activity of the aqueous extract of four Phyllanthus species were evaluated against herpes simplex virus type-1 (HSV-1) and HSV-2 in Vero cells by quantitative PCR. The protein expressions of untreated and treated infected Vero cells were studied by 2D-gel electrophoresis and Western blot. This is the first study that reported the antiviral activity of P. watsonii. P. urinaria was shown to demonstrate the strongest antiviral activity against HSV-1 and HSV-2, with SI > 33.6. Time-of-addition studies suggested that the extract may act against the early infection stage and the replication stage. Protein expression studies indicated that cellular proteins that are involved in maintaining cytoskeletal structure could be potential target for development of antiviral drugs. Preliminary findings indicated that P. urinaria demonstrated potent inhibitory activity against HSV. Hence, further studies such as in vivo evaluation are required for the development of effective antiherpetic drug. 2013 Article PeerReviewed Tan, W.C. and Jaganath, I.B. and Manikam, R. and Sekaran, S.D. (2013) Evaluation of antiviral activities of four local malaysian phyllanthus species against herpes simplex viruses and possible antiviral target. International Journal of Medical Sciences, 10 (13). pp. 1817-1829. ISSN 1449-1907,
spellingShingle R Medicine
Tan, W.C.
Jaganath, I.B.
Manikam, R.
Sekaran, S.D.
Evaluation of antiviral activities of four local malaysian phyllanthus species against herpes simplex viruses and possible antiviral target
title Evaluation of antiviral activities of four local malaysian phyllanthus species against herpes simplex viruses and possible antiviral target
title_full Evaluation of antiviral activities of four local malaysian phyllanthus species against herpes simplex viruses and possible antiviral target
title_fullStr Evaluation of antiviral activities of four local malaysian phyllanthus species against herpes simplex viruses and possible antiviral target
title_full_unstemmed Evaluation of antiviral activities of four local malaysian phyllanthus species against herpes simplex viruses and possible antiviral target
title_short Evaluation of antiviral activities of four local malaysian phyllanthus species against herpes simplex viruses and possible antiviral target
title_sort evaluation of antiviral activities of four local malaysian phyllanthus species against herpes simplex viruses and possible antiviral target
topic R Medicine
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